anti-Human Nuclear Cap Binding Protein Subunit 1, 80kDa antibody for Immunofluorescence

Recommended Nuclear Cap Binding Protein Subunit 1, 80kDa Antibody (supplied by: Log in to see )

Antigen
Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1) Antibodies
  • CBP80
  • CG7035
  • Dmel\\CG7035
  • EG:84H4.3
  • ncbp
  • sto1
  • cbp80
  • AU014645
  • AW538051
  • NCBP
  • Sto1
  • ncbp1
  • ABA HYPERSENSITIVE 1
  • ATCBP80
  • CAP-BINDING PROTEIN 80
  • ENS
  • ENSALADA
  • NUCLEAR CAP-BINDING PROTEIN CBP80
  • T10F5.8
  • T10F5_8
  • cap binding protein 80
  • nuclear cap binding protein subunit 1
  • nuclear cap binding protein subunit 1 S homeolog
  • nuclear cap binding protein subunit 1, 80kDa
  • nuclear cap binding protein subunit 1 L homeolog
  • ARM repeat superfamily protein
  • Nuclear cap-binding protein subunit 1-A
  • Nuclear cap-binding protein subunit 1
  • Cbp80
  • ncbp1
  • ncbp1.S
  • NCBP1
  • Ncbp1
  • ncbp1.L
  • ABH1
  • nbp1a
  • ncbp-1
Epitope
N-Term
16
13
8
3
3
3
2
2
1
1
1
1
Reactivity
Human
65
27
23
5
4
4
3
2
2
2
1
1
1
Host
Rabbit
58
7
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
63
41
18
7
7
6
4
1
Options
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Available images

Catalog No. ABIN2856327
$ 466.18
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
17.475594 ABIN518206 IF WB Mouse AA 1-790 Log in to see Polyclonal 0
14.4755945 ABIN518205 IF WB Mouse AA 1-790 Log in to see Polyclonal 0
11.31644 ABIN4338252 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
11.31644 ABIN4338251 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
7 ABIN6257658 ELISA ICC IF WB Rabbit IgG Log in to see Polyclonal 0
2.3164396 ABIN4338250 ICC IF IHC IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 0
1 ABIN1916275 IF IHC IHC (p) IP WB Rabbit IgG AA 1-50 Log in to see Polyclonal 0
1 ABIN1916277 IF IHC IHC (p) Rabbit IgG AA 482-532 Log in to see Polyclonal 0
1 ABIN1916276 IF IHC IHC (p) IP WB Rabbit IgG AA 482-532 Log in to see Polyclonal 0
1 ABIN2317835 IF WB Mouse IgG AA 1-790 Log in to see Polyclonal 0
-0.6835604 ABIN4338249 ELISA ICC IF WB Rabbit Center, all Isoforms Log in to see Polyclonal 0

General

Antigen Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1) Antibodies
Epitope N-Term
(16), (13), (8), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Reactivity Human
(65), (27), (23), (5), (4), (4), (3), (2), (2), (2), (1), (1), (1)
Host Rabbit
(58), (7)
Clonality
Polyclonal
Conjugate Un-conjugated
(2), (2), (2), (2), (2), (2)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(63), (41), (18), (7), (7), (6), (4), (1)
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Product details

Target Details Application Details Handling Images
Cross-Reactivity Mouse (Murine), Rat (Rattus), Xenopus tropicalis, Chicken
Cross-Reactivity (Details) Mouse (100 %), Rat (100 %), Xenopus Tropicalis (91 %), Chicken (100 %)
Characteristics Rabbit Polyclonal antibody to NCBP1 (nuclear cap binding protein subunit 1, 80 kDa)
NCBP1 antibody [N2], N-term
Purification Purified by antigen-affinity chromatography.
Immunogen Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human NCBP1. The exact sequence is proprietary.
Isotype IgG

Target Details

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Antigen
Alternative Name NCBP1 (NCBP1 Antibody Abstract)
Background The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay.

Cellular Localization: Nucleus
Molecular Weight 92 kDa
Gene ID 4686
Pathways Ribonucleoprotein Complex Subunit Organization, Photoperiodism, Methionine Biosynthetic Process

Application Details

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Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Restrictions For Research Use only

Handling

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Format Liquid
Concentration 1 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

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Supplier Images
Western Blotting (WB) image for anti-Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1) (N-Term) antibody (ABIN2856327) WB Image Sample (30 ug of whole cell lysate) A: A431 , 7.5% SDS PAGE antibody diluted...
Immunohistochemistry (IHC) image for anti-Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1) (N-Term) antibody (ABIN2856327) IHC-P Image Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using N...
Immunofluorescence (IF) image for anti-Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1) (N-Term) antibody (ABIN2856327) ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CBP80 ...