anti-Cat (Feline) Nucleolin antibody for Western Blotting

Recommended Nucleolin Antibody

Antigen
Nucleolin (NCL) Antibodies
  • C23
  • B530004O11Rik
  • D0Nds28
  • D1Nds28
  • Nucl
  • wu:fa12d03
  • zgc:152810
  • nucl
  • LOC100282923
  • LOC100283058
  • nucleolin
  • nucleolin S homeolog
  • NCL
  • Ncl
  • ncl.S
  • ncl
  • LOC100195537
  • LOC100282923
  • LOC100283058
Reactivity
Cat (Feline), Chicken, Cow (Bovine), Gerbil, Guinea Pig, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
234
62
37
11
11
10
9
9
7
6
5
4
3
1
1
1
1
1
1
1
Host
Mouse
121
121
1
Clonality
Monoclonal
Conjugate
This Nucleolin antibody is un-conjugated
17
11
10
9
9
9
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
214
131
125
88
63
58
38
24
21
17
13
9
2
1
1
Options

Available images

Catalog No. ABIN3025707
$ 471.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
19.877544 ABIN3025709 FACS IF IHC (p) WB Mouse IgG1 NCL-902 0
1 ABIN6290705 WB Goat IgG C-Term Polyclonal 0
1 ABIN6297426 FACS IF IHC WB Mouse 0

General

Antigen Nucleolin (NCL) Antibodies
Reactivity Cat (Feline), Chicken, Cow (Bovine), Gerbil, Guinea Pig, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(234), (62), (37), (11), (11), (10), (9), (9), (7), (6), (5), (4), (3), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(121), (121), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Nucleolin antibody is un-conjugated
(17), (11), (10), (9), (9), (9), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(214), (131), (125), (88), (63), (58), (38), (24), (21), (17), (13), (9), (2), (1), (1)
Supplier

Product Details anti-Nucleolin Antibody

Target Details Nucleolin Application Details Handling Images
Purification Protein G affinity chromatography
Immunogen Recombinant human protein was used as the immunogen for the Nucleolin antibody.
Clone NCL-902
Isotype IgG1
Plasmids, Primers & others

Target Details Nucleolin

Product Details anti-Nucleolin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Nucleolin (NCL Antibody Abstract)
Background Recognizes a protein of ~76  kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.
Pathways Ribonucleoprotein Complex Subunit Organization

Application Details

Product Details anti-Nucleolin Antibody Target Details Nucleolin Handling Images back to top
Application Notes Optimal dilution of the Nucleolin antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,IF: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Western blot: 0.5-1 μg/mL,Prediluted format: incubate for 30 min at RT (2)

Restrictions For Research Use only

Handling

Product Details anti-Nucleolin Antibody Target Details Nucleolin Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store the Nucleolin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-Nucleolin Antibody Target Details Nucleolin Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Nucleolin (NCL) antibody (ABIN3025707) Flow cytometry of human Jurkat cells. Grey: isotype control; Green: FITC-labeled; Pur...
Flow Cytometry (FACS) image for anti-Nucleolin (NCL) antibody (ABIN3025707) Flow cytometry testing of human 293T cells. Black: cells alone; Grey: isotype control...
Immunohistochemistry (IHC) image for anti-Nucleolin (NCL) antibody (ABIN3025707) Formalin-fixed, paraffin-embedded human testicular carcinoma stained with Nucleolin a...
Immunohistochemistry (IHC) image for anti-Nucleolin (NCL) antibody (ABIN3025707) Formalin-fixed, paraffin-embedded human tonsil stained with Nucleolin antibody.
Immunofluorescence (IF) image for anti-Nucleolin (NCL) antibody (ABIN3025707) HeLa cells stained with AF488 labeled Nucleolin antibody. Green: AF488-labeled Ab. Bl...
Western Blotting (WB) image for anti-Nucleolin (NCL) antibody (ABIN3025707) Western blot of A431 cell lysate using Nucleolin antibody.
 image for anti-Nucleolin (NCL) antibody (ABIN3025707) anti-Nucleolin (NCL) antibody (Image 7)
 image for anti-Nucleolin (NCL) antibody (ABIN3025707) anti-Nucleolin (NCL) antibody (Image 8)
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) image for anti-Nucleolin (NCL) antibody (ABIN3025707) Formalin-fixed, paraffin-embedded human uterus stained with Nucleolin antibody.