anti-Horse (Equine) Nucleolin antibody for Immunohistochemistry
Recommended Nucleolin Antibody
- Antigen
-
Nucleolin (NCL) Antibodies
show synonyms for this antigen- C23
- B530004O11Rik
- D0Nds28
- D1Nds28
- Nucl
- wu:fa12d03
- zgc:152810
- nucl
- LOC100282923
- LOC100283058
- nucleolin
- nucleolin S homeolog
- NCL
- Ncl
- ncl.S
- ncl
- LOC100195537
- LOC100282923
- LOC100283058
- Epitope
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C-Term
Alternatives - Reactivity
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Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
Alternatives - Host
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Rabbit
Alternatives - Clonality
- Conjugate
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This Nucleolin antibody is un-conjugated
Alternatives - Application
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Immunohistochemistry (IHC), Western Blotting (WB)
Alternatives - Options
Available images
Catalog No. ABIN2778936
$ 229.00
Plus shipping costs $45.00
Plus shipping costs $45.00
| Relevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Clonality | References | Details |
|---|---|---|---|---|---|---|---|---|---|
| 16.081745 | ABIN2778935 | IHC WB | Rabbit | N-Term | Polyclonal | 1 | |||
| 7 | ABIN202879 | IHC IHC (p) WB | Rabbit | IgG | C-Term | Polyclonal | 0 | ||
| 1 | ABIN1916332 | IF IHC IHC (p) IP WB | Rabbit | IgG | AA 550-710, C-Term | Polyclonal | 0 | ||
| 1 | ABIN1916330 | IF IHC IHC (p) IP WB | Rabbit | IgG | AA 250-300 | Polyclonal | 0 | ||
| 1 | ABIN1916331 | IF IHC IHC (p) IP WB | Rabbit | IgG | AA 525-575 | Polyclonal | 0 |
General |
|
|---|---|
| Antigen | Nucleolin (NCL) Antibodies |
| Epitope | C-Term Alternatives |
| Reactivity | Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus) Alternatives |
| Host | Rabbit Alternatives |
| Clonality | |
| Conjugate | This Nucleolin antibody is un-conjugated Alternatives |
| Application |
Immunohistochemistry (IHC), Western Blotting (WB)
Alternatives
|
|
1 reference available |
| Supplier | |
Product Details anti-Nucleolin AntibodyTarget Details Nucleolin Application Details Handling References for anti-Nucleolin antibody (ABIN2778936) Images |
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| Sequence | GGFGGRGGGR GGRGGFGGRG RGGFGGRGGF RGGRGGGGDH KPQGKKTKFE |
| Predicted Reactivity | Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100% |
| Characteristics | This is a rabbit polyclonal antibody against NCL. It was validated on Western Blot and immunohistochemistry. |
| Purification | Protein A purified |
| Immunogen | The immunogen is a synthetic peptide directed towards the C terminal region of human NCL |
| Plasmids, Primers & others | |
Target Details NucleolinProduct Details anti-Nucleolin Antibody Application Details Handling References for anti-Nucleolin antibody (ABIN2778936) Images back to top |
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| Antigen | |
| Alternative Name | NCL (NCL Antibody Abstract) |
| Background |
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus.Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. Alias Symbols: C23 Protein Interaction Partner: HUWE1, PIK3R1, PA2G4, NCL, HNRNPD, AURKA, MDM2, NFE4, CDKN1A, ADAP1, STAU1, ADAP2, UBC, NUP62, ZBTB1, ARFGEF1, RPA3, RPA2, RPA1, ERG, RNF2, SUZ12, EED, EZH2, BMI1, NPM3, EIF3G, XRCC5, PLOD2, NCBP1, MCM2, LMNA, PRMT1, HNRNPK, KCTD12, DNM1, ACLY, SRPK3, TAR Protein Size: 710 |
| Molecular Weight | 78 kDa |
| Gene ID | 4691 |
| NCBI Accession | NM_005381, NP_005372 |
| UniProt | P19338 |
| Pathways | Ribonucleoprotein Complex Subunit Organization |
Application DetailsProduct Details anti-Nucleolin Antibody Target Details Nucleolin Handling References for anti-Nucleolin antibody (ABIN2778936) Images back to top |
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| Application Notes | Optimal working dilutions should be determined experimentally by the investigator. |
| Comment |
Antigen size: 710 AA |
| Restrictions | For Research Use only |
HandlingProduct Details anti-Nucleolin Antibody Target Details Nucleolin Application Details References for anti-Nucleolin antibody (ABIN2778936) Images back to top |
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| Format | Liquid |
| Concentration | Lot specific |
| Buffer | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose. |
| Preservative | Sodium azide |
| Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Handling Advice | Avoid repeated freeze-thaw cycles. |
| Storage | -20 °C |
| Storage Comment | For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. |
References for anti-Nucleolin antibody (ABIN2778936)Product Details anti-Nucleolin Antibody Target Details Nucleolin Application Details Handling Images back to top |
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| Background publications |
Huang, Shi, Zhou, Song, Yuan, Luo: "The angiogenic function of nucleolin is mediated by vascular endothelial growth factor and nonmuscle myosin." in: Blood, Vol. 107, Issue 9, pp. 3564-71, 2006 |
ImagesProduct Details anti-Nucleolin Antibody Target Details Nucleolin Application Details Handling References for anti-Nucleolin antibody (ABIN2778936) back to top |
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| Supplier Images |
WB Suggested Anti-NCL Antibody Titration: 1.25ug/ml Positive Control: Daudi cell l...
Sample Type: 1. Human NT-2 cells (60ug) Primary Antibody Dilution: 2ug/ml Secondary A...
Sample Type: NT2 cells Red: Antibody Blue: DAPI Primary Dilution: 1ug/50ul antibody S...
Sample Type : Human brain stem cells Primary Antibody Dilution : 1:500 Secondary ...
Rabbit Anti-NCL Antibody Paraffin Embedded Tissue: Human Kidney Cellular Data: Epith...
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