anti-Horse (Equine) Nucleolin antibody for Immunohistochemistry

Recommended Nucleolin Antibody

Antigen
Nucleolin (NCL) Antibodies
  • C23
  • B530004O11Rik
  • D0Nds28
  • D1Nds28
  • Nucl
  • wu:fa12d03
  • zgc:152810
  • nucl
  • LOC100282923
  • LOC100283058
  • nucleolin
  • nucleolin S homeolog
  • NCL
  • Ncl
  • ncl.S
  • ncl
  • LOC100195537
  • LOC100282923
  • LOC100283058
Epitope
C-Term
14
14
11
10
9
8
7
7
7
7
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
230
61
37
11
11
10
9
9
7
6
6
5
3
1
1
1
1
1
1
1
Host
Rabbit
122
115
1
Clonality
Polyclonal
Conjugate
This Nucleolin antibody is un-conjugated
15
10
9
9
9
8
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
209
132
124
89
63
58
39
24
21
17
13
9
2
1
1
Options

Available images

Catalog No. ABIN2778936
$ 229.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
16.093842 ABIN2778935 IHC WB Rabbit N-Term Polyclonal 1
7 ABIN202879 IHC IHC (p) WB Rabbit IgG C-Term Polyclonal 0
1 ABIN1916332 IF IHC IHC (p) IP WB Rabbit IgG AA 550-710, C-Term Polyclonal 0
1 ABIN1916330 IF IHC IHC (p) IP WB Rabbit IgG AA 250-300 Polyclonal 0
1 ABIN1916331 IF IHC IHC (p) IP WB Rabbit IgG AA 525-575 Polyclonal 0

General

Antigen Nucleolin (NCL) Antibodies
Epitope C-Term
(14), (14), (11), (10), (9), (8), (7), (7), (7), (7), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
(230), (61), (37), (11), (11), (10), (9), (9), (7), (6), (6), (5), (3), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(122), (115), (1)
Clonality
Polyclonal
Conjugate This Nucleolin antibody is un-conjugated
(15), (10), (9), (9), (9), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(209), (132), (124), (89), (63), (58), (39), (24), (21), (17), (13), (9), (2), (1), (1)
Pubmed 1 reference available
Supplier

Product Details anti-Nucleolin Antibody

Target Details Nucleolin Application Details Handling References for anti-Nucleolin antibody (ABIN2778936) Images
Sequence GGFGGRGGGR GGRGGFGGRG RGGFGGRGGF RGGRGGGGDH KPQGKKTKFE
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against NCL. It was validated on Western Blot and immunohistochemistry.
Purification Protein A purified
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human NCL
Plasmids, Primers & others

Target Details Nucleolin

Product Details anti-Nucleolin Antibody Application Details Handling References for anti-Nucleolin antibody (ABIN2778936) Images back to top
Antigen
Alternative Name NCL (NCL Antibody Abstract)
Background Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus.Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
Alias Symbols: C23
Protein Interaction Partner: HUWE1, PIK3R1, PA2G4, NCL, HNRNPD, AURKA, MDM2, NFE4, CDKN1A, ADAP1, STAU1, ADAP2, UBC, NUP62, ZBTB1, ARFGEF1, RPA3, RPA2, RPA1, ERG, RNF2, SUZ12, EED, EZH2, BMI1, NPM3, EIF3G, XRCC5, PLOD2, NCBP1, MCM2, LMNA, PRMT1, HNRNPK, KCTD12, DNM1, ACLY, SRPK3, TAR
Protein Size: 710
Molecular Weight 78 kDa
Gene ID 4691
NCBI Accession NM_005381, NP_005372
UniProt P19338
Pathways Ribonucleoprotein Complex Subunit Organization

Application Details

Product Details anti-Nucleolin Antibody Target Details Nucleolin Handling References for anti-Nucleolin antibody (ABIN2778936) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 710 AA

Restrictions For Research Use only

Handling

Product Details anti-Nucleolin Antibody Target Details Nucleolin Application Details References for anti-Nucleolin antibody (ABIN2778936) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Nucleolin antibody (ABIN2778936)

Product Details anti-Nucleolin Antibody Target Details Nucleolin Application Details Handling Images back to top
Background publications

Huang, Shi, Zhou, Song, Yuan, Luo: "The angiogenic function of nucleolin is mediated by vascular endothelial growth factor and nonmuscle myosin." in: Blood, Vol. 107, Issue 9, pp. 3564-71, 2006

Images

Product Details anti-Nucleolin Antibody Target Details Nucleolin Application Details Handling References for anti-Nucleolin antibody (ABIN2778936) back to top
Supplier Images
Western Blotting (WB) image for anti-Nucleolin (NCL) (C-Term) antibody (ABIN2778936) WB Suggested Anti-NCL Antibody Titration: 1.25ug/ml Positive Control: Daudi cell l...
Western Blotting (WB) image for anti-Nucleolin (NCL) (C-Term) antibody (ABIN2778936) Sample Type: 1. Human NT-2 cells (60ug) Primary Antibody Dilution: 2ug/ml Secondary A...
Immunohistochemistry (IHC) image for anti-Nucleolin (NCL) (C-Term) antibody (ABIN2778936) Sample Type: NT2 cells Red: Antibody Blue: DAPI Primary Dilution: 1ug/50ul antibody S...
Immunohistochemistry (IHC) image for anti-Nucleolin (NCL) (C-Term) antibody (ABIN2778936) Sample Type : Human brain stem cells Primary Antibody Dilution : 1:500 Secondary ...
Immunohistochemistry (IHC) image for anti-Nucleolin (NCL) (C-Term) antibody (ABIN2778936) Rabbit Anti-NCL Antibody Paraffin Embedded Tissue: Human Kidney Cellular Data: Epith...