anti-Human Nucleolin antibody for Immunohistochemistry (Formalin-fixed Sections)

Recommended Nucleolin Antibody

Antigen
Nucleolin (NCL) Antibodies
  • C23
  • B530004O11Rik
  • D0Nds28
  • D1Nds28
  • Nucl
  • wu:fa12d03
  • zgc:152810
  • nucl
  • LOC100282923
  • LOC100283058
  • nucleolin
  • nucleolin S homeolog
  • NCL
  • Ncl
  • ncl.S
  • ncl
  • LOC100195537
  • LOC100282923
  • LOC100283058
Reactivity
Human
230
62
38
12
11
11
10
10
7
7
6
5
3
1
1
1
1
1
1
1
Host
Mouse
121
116
1
Clonality
Monoclonal
Conjugate
This Nucleolin antibody is conjugated to PerCP
15
10
9
9
9
8
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunostaining (ISt), Western Blotting (WB)
209
131
125
88
62
58
39
24
20
17
13
9
2
1
1
Options

Available images

Catalog No. ABIN6166102
$ 441.85
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
13 ABIN6166103 FACS IF IHC (f) ISt WB PE Mouse IgG1, kappa 364-5 0
13 ABIN6166095 FACS IF IHC (f) ISt WB APC Mouse IgG1, kappa 364-5 0
13 ABIN6166106 FACS IF IHC (f) ISt WB Mouse IgG1, kappa 364-5 0
1 ABIN6166069 FACS IF IHC (f) ISt WB CF®405S Mouse IgG1, kappa 364-5 0
1 ABIN6166081 FACS IF IHC (f) ISt WB CF®660R Mouse IgG1, kappa 364-5 0
1 ABIN6166089 FACS IF IHC (f) ISt WB CF®680R Mouse IgG1, kappa 364-5 0
1 ABIN6166085 FACS IF IHC (f) ISt WB CF®770 Mouse IgG1, kappa 364-5 0
1 ABIN6166071 FACS IF IHC (f) ISt WB CF405M Mouse IgG1, kappa 364-5 0
1 ABIN6166091 FACS IF IHC (f) ISt WB CF®488A Mouse IgG1, kappa 364-5 0
1 ABIN6166075 FACS IF IHC (f) ISt WB CF®543 Mouse IgG1, kappa 364-5 0
1 ABIN6166079 FACS IF IHC (f) ISt WB CF®555 Mouse IgG1, kappa 364-5 0
1 ABIN6166083 FACS IF IHC (f) ISt WB CF®568 Mouse IgG1, kappa 364-5 0
1 ABIN6166093 FACS IF IHC (f) ISt WB CF®594 Mouse IgG1, kappa 364-5 0
1 ABIN6166073 FACS IF IHC (f) ISt WB CF®640R Mouse IgG1, kappa 364-5 0
1 ABIN6166077 FACS IF IHC (f) ISt WB CF®647 Mouse IgG1, kappa 364-5 0
1 ABIN6166087 FACS IF IHC (f) ISt WB CF®680 Mouse IgG1, kappa 364-5 0
1 ABIN6166104 FACS IF IHC (f) ISt WB Mouse IgG1, kappa 364-5 0
1 ABIN6166096 FACS IF IHC (f) ISt WB Alkaline Phosphatase (AP) Mouse IgG1, kappa 364-5 0
1 ABIN6166098 FACS IF IHC (f) ISt WB Biotin Mouse IgG1, kappa 364-5 0
1 ABIN6166100 FACS IF IHC (f) ISt WB HRP Mouse IgG1, kappa 364-5 0

General

Antigen Nucleolin (NCL) Antibodies
Reactivity Human
(230), (62), (38), (12), (11), (11), (10), (10), (7), (7), (6), (5), (3), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(121), (116), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Nucleolin antibody is conjugated to PerCP
(15), (10), (9), (9), (9), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunostaining (ISt), Western Blotting (WB)
(209), (131), (125), (88), (62), (58), (39), (24), (20), (17), (13), (9), (2), (1), (1)
Supplier

Product Details anti-Nucleolin Antibody

Target Details Nucleolin Application Details Handling Images
Purpose Mouse Monoclonal anti-Nucleolin (364-5), Per-CP Conjugate
Characteristics This antibody recognizes a protein of ~76 kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Immunogen Lysate of SU-DHL-1 Nuclei
Clone 364-5
Isotype IgG1, kappa
Plasmids, Primers & others

Target Details Nucleolin

Product Details anti-Nucleolin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Nucleolin (NCL Antibody Abstract)
Background NCL, Nucl, Nucleolin, Protein C23
Molecular Weight 76 kDa
Gene ID 4691, 79110
UniProt P19338
Pathways Ribonucleoprotein Complex Subunit Organization

Application Details

Product Details anti-Nucleolin Antibody Target Details Nucleolin Handling Images back to top
Application Notes Does not react with mouse, rat or cow, others not known
  • Immunohistology formalin-fixed 0.25-0.5 μg/mL
  • Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
  • Immunofluorescence 0.5-1 μg/mL
  • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
  • Western blotting 0.5-1 μg/mL
  • Optimal dilution for a specific application should be determined by user
Comment

All human cells

Restrictions For Research Use only

Handling

Product Details anti-Nucleolin Antibody Target Details Nucleolin Application Details Images back to top
Format Liquid
Concentration 100 μg/mL
Buffer PBS/0.1 % BSA/0.05 % azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Images

Product Details anti-Nucleolin Antibody Target Details Nucleolin Application Details Handling back to top
Supplier Images
 image for anti-Nucleolin (NCL) antibody (PerCP) (ABIN6166102) anti-Nucleolin (NCL) antibody (PerCP)
 image for anti-Nucleolin (NCL) antibody (PerCP) (ABIN6166102) anti-Nucleolin (NCL) antibody (PerCP) (Image 2)
 image for anti-Nucleolin (NCL) antibody (PerCP) (ABIN6166102) anti-Nucleolin (NCL) antibody (PerCP) (Image 3)
 image for anti-Nucleolin (NCL) antibody (PerCP) (ABIN6166102) anti-Nucleolin (NCL) antibody (PerCP) (Image 4)