anti-Zebrafish (Danio rerio) Nucleolin antibody for Western Blotting

Recommended Nucleolin Antibody

Antigen
Nucleolin (NCL) Antibodies
  • C23
  • B530004O11Rik
  • D0Nds28
  • D1Nds28
  • Nucl
  • wu:fa12d03
  • zgc:152810
  • nucl
  • LOC100282923
  • LOC100283058
  • nucleolin
  • nucleolin S homeolog
  • NCL
  • Ncl
  • ncl.S
  • ncl
  • LOC100195537
  • LOC100282923
  • LOC100283058
Epitope
N-Term
14
14
11
10
9
8
8
7
7
7
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Yeast (Saccharomyces cerevisiae), Zebrafish (Danio rerio)
230
61
37
11
10
10
10
9
7
6
6
5
3
1
1
1
1
1
Host
Rabbit
122
115
1
Clonality
Polyclonal
Conjugate
This Nucleolin antibody is un-conjugated
15
10
9
9
9
8
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
209
132
124
89
63
58
39
24
21
17
13
9
2
1
1
Options

Available images

Catalog No. ABIN2778935
$ 289.00
Plus shipping costs $45.00

General

Antigen Nucleolin (NCL) Antibodies
Epitope N-Term
(14), (14), (11), (10), (9), (8), (8), (7), (7), (7), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Yeast (Saccharomyces cerevisiae), Zebrafish (Danio rerio)
(230), (61), (37), (11), (10), (10), (10), (9), (7), (6), (6), (5), (3), (1), (1), (1), (1), (1)
Host Rabbit
(122), (115), (1)
Clonality
Polyclonal
Conjugate This Nucleolin antibody is un-conjugated
(15), (10), (9), (9), (9), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(209), (132), (124), (89), (63), (58), (39), (24), (21), (17), (13), (9), (2), (1), (1)
Pubmed 1 reference available
Supplier

Product Details anti-Nucleolin Antibody

Target Details Nucleolin Application Details Handling References for anti-Nucleolin antibody (ABIN2778935) Images
Blocking Peptide Blocking peptide for this product available: ABIN980529
Sequence GKALVATPGK KGAAIPAKGA KNGKNAKKED SDEEEDDDSE EDEEDDEDED
Predicted Reactivity Cow: 87%, Dog: 93%, Horse: 93%, Human: 100%, Mouse: 91%, Pig: 86%, Rabbit: 77%, Rat: 93%, Yeast: 83%, Zebrafish: 85%
Characteristics This is a rabbit polyclonal antibody against NCL. It was validated on Western Blot and immunohistochemistry.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human NCL
Plasmids, Primers & others

Target Details Nucleolin

Product Details anti-Nucleolin Antibody Application Details Handling References for anti-Nucleolin antibody (ABIN2778935) Images back to top
Antigen
Alternative Name NCL (NCL Antibody Abstract)
Background Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus.Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
Alias Symbols: C23
Protein Interaction Partner: HUWE1, PIK3R1, PA2G4, NCL, HNRNPD, AURKA, MDM2, NFE4, CDKN1A, ADAP1, STAU1, ADAP2, UBC, NUP62, ZBTB1, ARFGEF1, RPA3, RPA2, RPA1, ERG, RNF2, SUZ12, EED, EZH2, BMI1, NPM3, EIF3G, XRCC5, PLOD2, NCBP1, MCM2, LMNA, PRMT1, HNRNPK, KCTD12, DNM1, ACLY, SRPK3, TAR
Protein Size: 710
Molecular Weight 78 kDa
Gene ID 4691
NCBI Accession NM_005381, NP_005372
UniProt P19338
Pathways Ribonucleoprotein Complex Subunit Organization

Application Details

Product Details anti-Nucleolin Antibody Target Details Nucleolin Handling References for anti-Nucleolin antibody (ABIN2778935) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 710 AA

Restrictions For Research Use only

Handling

Product Details anti-Nucleolin Antibody Target Details Nucleolin Application Details References for anti-Nucleolin antibody (ABIN2778935) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Nucleolin antibody (ABIN2778935)

Product Details anti-Nucleolin Antibody Target Details Nucleolin Application Details Handling Images back to top
Background publications

Huang, Shi, Zhou, Song, Yuan, Luo: "The angiogenic function of nucleolin is mediated by vascular endothelial growth factor and nonmuscle myosin." in: Blood, Vol. 107, Issue 9, pp. 3564-71, 2006

Images

Product Details anti-Nucleolin Antibody Target Details Nucleolin Application Details Handling References for anti-Nucleolin antibody (ABIN2778935) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Nucleolin (NCL) (N-Term) antibody (ABIN2778935) Human Skin
Immunohistochemistry (IHC) image for anti-Nucleolin (NCL) (N-Term) antibody (ABIN2778935) Rabbit Anti-NCL Antibody Catalog Number: ARP40583 Paraffin Embedded Tissue: Human Kid...
Immunohistochemistry (IHC) image for anti-Nucleolin (NCL) (N-Term) antibody (ABIN2778935) Rabbit Anti-NCL Antibody Catalog Number: ARP40583 Paraffin Embedded Tissue: Human Lun...
Western Blotting (WB) image for anti-Nucleolin (NCL) (N-Term) antibody (ABIN2778935) WB Suggested Anti-NCL Antibody Titration: 0.2-1 ug/mlPositive Control: Jurkat NCL is ...