anti-Human Angiopoietin 2 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Angiopoietin 2 Antibody (supplied by: Log in to see )

Antigen
Angiopoietin 2 (ANGPT2) Antibodies
  • AGPT2
  • ANG2
  • Agpt2
  • Ang-2
  • Ang2
  • ang2
  • angpt2
  • ANGIOPOIETIN-2
  • ANG-2
  • ANGPT2
  • angiopoietin 2
  • angiopoietin 2b
  • ANGPT2
  • Angpt2
  • angpt2b
Epitope
AA 404-432, C-Term
18
15
7
7
6
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
122
67
43
21
18
5
4
3
3
2
1
1
1
1
Host
Rabbit
119
34
6
2
1
1
Clonality
Polyclonal
Conjugate
This Angiopoietin 2 antibody is un-conjugated
13
8
7
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
120
80
64
34
27
23
21
19
19
13
8
6
2
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Catalog No. ABIN654062
$ 335.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
21.755764 ABIN2854898 ICC IF IHC (p) WB Rabbit IgG Center Log in to see Polyclonal 0
18.755764 ABIN2854896 IHC (p) WB Rabbit IgG Center Log in to see Polyclonal 0
14.5 ABIN3043725 IHC (p) Rabbit IgG AA 19-348 Log in to see Polyclonal 3
14.117413 ABIN950412 EIA IHC (p) WB Rabbit Ig Fraction AA 409-440, C-Term Log in to see Polyclonal 3
11.5 ABIN5518732 ELISA IHC (p) WB Rabbit IgG AA 180-283 Log in to see Polyclonal 3
11.5 ABIN671781 IF (p) IHC (p) WB Rabbit IgG AA 450-490 Log in to see Polyclonal 2
11.117413 ABIN1105366 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
7 ABIN5572509 ELISA IHC (p) WB Rabbit Log in to see Polyclonal 0
1 ABIN5556577 EIA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN671790 IHC (p) WB HRP Rabbit IgG AA 450-490 Log in to see Polyclonal 0
1 ABIN671783 IHC (p) WB Biotin Rabbit IgG AA 450-490 Log in to see Polyclonal 0
1 ABIN5647041 ELISA IHC (p) WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen Angiopoietin 2 (ANGPT2) Antibodies
Epitope AA 404-432, C-Term
(18), (15), (7), (7), (6), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(122), (67), (43), (21), (18), (5), (4), (3), (3), (2), (1), (1), (1), (1)
Host Rabbit
(119), (34), (6), (2), (1), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This Angiopoietin 2 antibody is un-conjugated
(13), (8), (7), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(120), (80), (64), (34), (27), (23), (21), (19), (19), (13), (8), (6), (2)
Pubmed 3 references available
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Product Details anti-Angiopoietin 2 Antibody

Target Details Angiopoietin 2 Application Details Handling References for anti-Angiopoietin 2 antibody (ABIN654062) Images
Specificity This ANGPT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 409-440 amino acids from the C-terminal region of human ANGPT2.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This ANGPT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 404-432 AA from the C-terminal region of human ANGPT2.
Clone RB17789
Isotype Ig Fraction
Plasmids, Primers & others

Target Details Angiopoietin 2

Product Details anti-Angiopoietin 2 Antibody Application Details Handling References for anti-Angiopoietin 2 antibody (ABIN654062) Images back to top
Antigen
Alternative Name ANGPT2 (ANGPT2 Antibody Abstract)
Background The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene.
Synonyms: Angiopoietin-2,ANGPT2,
Molecular Weight 56919 DA
Gene ID 285
NCBI Accession NP_001112360, NP_001138, NP_001112359
UniProt O15123
Pathways RTK Signaling

Application Details

Product Details anti-Angiopoietin 2 Antibody Target Details Angiopoietin 2 Handling References for anti-Angiopoietin 2 antibody (ABIN654062) Images back to top
Application Notes IHC (p) = 1:50-100, FACS = 1:10-50, WB = 1:1000
Restrictions For Research Use only

Handling

Product Details anti-Angiopoietin 2 Antibody Target Details Angiopoietin 2 Application Details References for anti-Angiopoietin 2 antibody (ABIN654062) Images back to top
Format Liquid
Concentration 0.46 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-Angiopoietin 2 antibody (ABIN654062)

Product Details anti-Angiopoietin 2 Antibody Target Details Angiopoietin 2 Application Details Handling Images back to top
Product cited in:

Alamo, Gallardo, Di Nicolantonio, Pavón, Casanova, Trias, Mangues, Lopez-Pousa, Villaverde, Vázquez, Bardelli, Céspedes, Mangues: "Higher metastatic efficiency of KRas G12V than KRas G13D in a colorectal cancer model." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 29, Issue 2, pp. 464-76, 2015 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Alamo, Gallardo, Pavón, Casanova, Trias, Mangues, Vázquez, Villaverde, Mangues, Céspedes: "Subcutaneous preconditioning increases invasion and metastatic dissemination in mouse colorectal cancer models." in: Disease models & mechanisms, Vol. 7, Issue 3, pp. 387-96, 2014 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Background publications

Morrissey, Dowell, Koreckij, Nguyen, Lakely, Fanslow, True, Corey, Vessella: "Inhibition of angiopoietin-2 in LuCaP 23.1 prostate cancer tumors decreases tumor growth and viability." in: The Prostate, Vol. 70, Issue 16, pp. 1799-808, 2010

Images

Product Details anti-Angiopoietin 2 Antibody Target Details Angiopoietin 2 Application Details Handling References for anti-Angiopoietin 2 antibody (ABIN654062) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Angiopoietin 2 (ANGPT2) (AA 404-432), (C-Term) antibody (ABIN654062) ANGPT2 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffi...
Flow Cytometry (FACS) image for anti-Angiopoietin 2 (ANGPT2) (AA 404-432), (C-Term) antibody (ABIN654062) ANGPT2 Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) co...
Western Blotting (WB) image for anti-Angiopoietin 2 (ANGPT2) (AA 404-432), (C-Term) antibody (ABIN654062) ANGPT2 Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane...
Western Blotting (WB) image for anti-Angiopoietin 2 (ANGPT2) (AA 404-432), (C-Term) antibody (ABIN654062) ANGPT2 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/...
Flow Cytometry (FACS) image for anti-Angiopoietin 2 (ANGPT2) (AA 404-432), (C-Term) antibody (ABIN654062) Overlay histogram showing A549 cells stained with ABIN2843960 (green line). The cells...
Western Blotting (WB) image for anti-Angiopoietin 2 (ANGPT2) (AA 404-432), (C-Term) antibody (ABIN654062) Anti-ANGPT2 Antibody (C-term) at 1:2000 dilution + human liver lysate Lysates/protein...