anti-Human Angiopoietin 2 antibody for Western Blotting

Recommended Angiopoietin 2 Antibody (supplied by: Log in to see )

Antigen
Angiopoietin 2 (ANGPT2) Antibodies
  • AGPT2
  • ANG2
  • Agpt2
  • Ang-2
  • Ang2
  • ang2
  • angpt2
  • ANGIOPOIETIN-2
  • ANG-2
  • ANGPT2
  • angiopoietin 2
  • angiopoietin 2b
  • ANGPT2
  • Angpt2
  • angpt2b
Epitope
N-Term
20
15
8
7
5
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
125
70
46
21
18
5
4
3
3
2
1
1
1
1
Host
Rabbit
124
33
6
2
1
1
Clonality
Polyclonal
Conjugate
This Angiopoietin 2 antibody is un-conjugated
13
8
7
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
124
82
65
36
27
24
21
19
19
13
8
7
2
Options
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Available images

Catalog No. ABIN95113
$ 637.50
Plus shipping costs $45.00 and $22.60 dry ice
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
25.196217 ABIN3021112 IHC WB Rabbit IgG Log in to see Polyclonal 0
24.234901 ABIN654062 FACS IHC (p) WB Rabbit Ig Fraction AA 404-432, C-Term Log in to see Polyclonal 3
22.196217 ABIN2854898 ICC IF IHC (p) WB Rabbit IgG Center Log in to see Polyclonal 0
19.196217 ABIN2854896 IHC (p) WB Rabbit IgG Center Log in to see Polyclonal 0
13.734901 ABIN1171925 ICC IHC WB Rabbit IgG AA 24-165 Log in to see Polyclonal 0
13.734901 ABIN2907611 IF/ICC IHC IP WB Rabbit AA 24-165 Log in to see Polyclonal 0
12.461315 ABIN5618795 WB Rabbit Center Log in to see Polyclonal 0
12.461315 ABIN2133685 WB Rabbit IgG C-Term Log in to see Polyclonal 0
12.234901 ABIN3043679 WB Rabbit IgG AA 454-468, C-Term Log in to see Polyclonal 3
11.5 ABIN5518732 ELISA IHC (p) WB Rabbit IgG AA 180-283 Log in to see Polyclonal 3
11.5 ABIN671781 IF (p) IHC (p) WB Rabbit IgG AA 450-490 Log in to see Polyclonal 2
10.734901 ABIN950412 EIA IHC (p) WB Rabbit Ig Fraction AA 409-440, C-Term Log in to see Polyclonal 3
10.734901 ABIN6259928 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
10.734901 ABIN1870971 IHC WB Rabbit IgG Log in to see Polyclonal 0
10.734901 ABIN1857996 ICC IHC WB Rabbit IgG AA 269-496 Log in to see Polyclonal 0
10.734901 ABIN2907612 IF/ICC IHC IP WB Rabbit AA 269-496 Log in to see Polyclonal 0
10.734901 ABIN2421171 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
7.7349014 ABIN1105366 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
7.7349014 ABIN2988829 IF/ICC WB Rabbit IgG Log in to see Polyclonal 0
7.7349014 ABIN6566651 IF WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen Angiopoietin 2 (ANGPT2) Antibodies
Epitope N-Term
(20), (15), (8), (7), (5), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(125), (70), (46), (21), (18), (5), (4), (3), (3), (2), (1), (1), (1), (1)
Host Rabbit
(124), (33), (6), (2), (1), (1)
Clonality
Polyclonal
Conjugate This Angiopoietin 2 antibody is un-conjugated
(13), (8), (7), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(124), (82), (65), (36), (27), (24), (21), (19), (19), (13), (8), (7), (2)
Pubmed 1 reference available
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Product Details anti-Angiopoietin 2 Antibody

Target Details Angiopoietin 2 Application Details Handling References for anti-Angiopoietin 2 antibody (ABIN95113) Images
Specificity This antiserum is directed against mouse angiopoietin-2 and shows no reactivity with angiopoietin-1 from mouse-derived proteins. A 75 kDa band is detected when assayed by immunoblot. This reagent cross-reacts with human angiopoietin-2. Partially cross-reactivity is noted with human angiopoietin-1.
Characteristics Anti Antiopoietin-2 Antibody recognizes Angiopoietin-2 (Ang-2) that has importance in development of the endothelium through regulation of tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding and thus blocks receptor phosphorylation. Ang-2 expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.
Sterility Sterile filtered
Immunogen This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide, corresponding to a region near the N-terminus of mouse angiopoietin-2 protein, conjugated to KLH using maleimide.
Immunogen Type: Peptide
Plasmids, Primers & others

Target Details Angiopoietin 2

Product Details anti-Angiopoietin 2 Antibody Application Details Handling References for anti-Angiopoietin 2 antibody (ABIN95113) Images back to top
Antigen
Alternative Name Angiopoietin 2 (ANGPT2 Antibody Abstract)
Background Anti Antiopoietin-2 Antibody recognizes Angiopoietin-2 (Ang-2) that has importance in development of the endothelium through regulation of tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding and thus blocks receptor phosphorylation. Ang-2 expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.
Synonyms: AGPT 2 antibody, Agpt2 antibody, ANG 2 antibody, ANG2 antibody, Angiopoietin 2a antibody, Angiopoietin 2B antibody, Angiopoietin2 antibody, ANGPT 2 antibody, ANGPT2 antibody, Tie2 ligand antibody
Gene ID 11601
UniProt O35608
Pathways RTK Signaling

Application Details

Product Details anti-Angiopoietin 2 Antibody Target Details Angiopoietin 2 Handling References for anti-Angiopoietin 2 antibody (ABIN95113) Images back to top
Application Notes Angiopoietin 2 Antibody is suitable for western blotting and other antibody based assays. A 1:500 dilution is recommended for western blotting. The reaction of this antiserum directly with cell supernatants may result in high background due to reactivity of components in the serum. This can be alleviated by first immunoprecipitating the antibody:antigen complex and then detecting the antigen. This method results in a very clean, strong signal.
Comment

Gene Name: ANG-2

Restrictions For Research Use only

Handling

Product Details anti-Angiopoietin 2 Antibody Target Details Angiopoietin 2 Application Details References for anti-Angiopoietin 2 antibody (ABIN95113) Images back to top
Format Liquid
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Expiry Date 12 months

References for anti-Angiopoietin 2 antibody (ABIN95113)

Product Details anti-Angiopoietin 2 Antibody Target Details Angiopoietin 2 Application Details Handling Images back to top
Product cited in:

Davis, Aldrich, Jones, Acheson, Compton, Jain, Ryan, Bruno, Radziejewski, Maisonpierre, Yancopoulos: "Isolation of angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning." in: Cell, Vol. 87, Issue 7, pp. 1161-9, 1997

Images

Product Details anti-Angiopoietin 2 Antibody Target Details Angiopoietin 2 Application Details Handling References for anti-Angiopoietin 2 antibody (ABIN95113) back to top
Supplier Images
Western Blotting (WB) image for anti-Angiopoietin 2 (ANGPT2) (N-Term) antibody (ABIN95113) Rabbit anti-Ang-2 was used at a 1:500 dilution to detect mouse Ang-2 in supernatants ...
 image for anti-Angiopoietin 2 (ANGPT2) (N-Term) antibody (ABIN95113) Immunocytochemistry of anti-ANG2 Antibody in PC12 cells Tissue: Human prostate cancer...
Immunohistochemistry (IHC) image for anti-Angiopoietin 2 (ANGPT2) (N-Term) antibody (ABIN95113) Immunohistochemistry of anti-ANG2 Antibody in human placenta Tissue: human placenta F...