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|Antigen||Betacellulin (BTC) Antibodies|
|Conjugate||This Betacellulin antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut), ELISA, Western Blotting (WB)
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Product Details anti-Betacellulin AntibodyTarget Details Betacellulin Application Details Handling Images
E.coli derived recombinant Human Betacellulin.
Type of Immunogen: Cell extract
Target Details BetacellulinProduct Details anti-Betacellulin Antibody Application Details Handling Images back to top
|Alternative Name||BTC / Betacellulin (BTC Antibody Abstract)|
Name/Gene ID: BTC
Synonyms: BTC, Betacellulin, Probetacellulin
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-Betacellulin Antibody Target Details Betacellulin Handling Images back to top
Approved: ELISA, IHC-P (5 μg/mL), Neut (0.1 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: LS-B7481 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human Betacellulin (0.1 ng/mL), a concentration of &le. 0.1 μg/mL of this antibody is required. ELISA: To detect hBetacellulin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Betacellulin (LS-C104290) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBetacellulin. Western Blot: To detect hBetacellulin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBetacellulin is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Betacellulin Antibody Target Details Betacellulin Application Details Images back to top
|Reconstitution||Reconstitute in sterile water|
|Buffer||Lyophilized from PBS, pH 7.2|
|Handling Advice||Avoid freeze/thaw cycles.|
|Storage||4 °C,-20 °C|
Store Lyophilized at room temperature up to 1 month
Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
ImagesProduct Details anti-Betacellulin Antibody Target Details Betacellulin Application Details Handling back to top