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|Antigen||Colony Stimulating Factor 1 Receptor (CSF1R) Antibodies|
|Reactivity||Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CSF1R antibody is un-conjugated Alternatives|
Functional Studies (Func), Flow Cytometry (FACS)
|3 references available|
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Product Details anti-CSF1R AntibodyTarget Details CSF1R Application Details Handling References for anti-CSF1R antibody (ABIN317381) Images
|Specificity||This antibody recognizes the Murine CD115 cell surface antigen, also known as the M-CSF receptor and as c-fms. This antibody has been shown to inhibit in vitro colony formation in response to M-CSF in both rats and mice.|
|Characteristics||Synonyms: CSF-1-R, Fms proto-oncogene, c-fms, CSF1R, FMS, Macrophage colony-stimulating factor 1receptor|
|Purification||Purified IgG prepared by ion exchange chromatography|
|Immunogen||RAW 264 cells. Spleen cells from immunised Sprague Dawley rats were fused with cells of the P3-653myeloma cell line.|
Target Details CSF1RProduct Details anti-CSF1R Antibody Application Details Handling References for anti-CSF1R antibody (ABIN317381) Images back to top
|Alternative Name||CD115 (CSF1R Antibody Abstract)|
|Background||This protein tyrosine kinase transmembrane receptor is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a member of the CSF1/PDGF receptor family of tyrosine protein kinases and contains 5 immunoglobulin like C2 type domains. CD115 is expressed by cells of the monocytic lineage and by progenitor cells. Mutations in this gene have been associated with a predisposition to myeloid malignancy.Synonyms: CSF-1-R, CSF1R, FMS, Fms proto-oncogene, Macrophage colony-stimulating factor 1 receptor, c-fms|
|Pathways||RTK Signaling, Inositol Metabolic Process, Cell-Cell Junction Organization|
Application DetailsProduct Details anti-CSF1R Antibody Target Details CSF1R Handling References for anti-CSF1R antibody (ABIN317381) Images back to top
Functional Assays. Flow Cytometry: Use 10 μL of 1/10-1/100 diluted antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CSF1R Antibody Target Details CSF1R Application Details References for anti-CSF1R antibody (ABIN317381) Images back to top
|Buffer||PBS, pH 7.4, None, None|
Store the antibody undiluted at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-CSF1R antibody (ABIN317381)Product Details anti-CSF1R Antibody Target Details CSF1R Application Details Handling Images back to top
MacDonald, Rowe, Bofinger, Thomas, Sasmono, Hume, Hill: "The colony-stimulating factor 1 receptor is expressed on dendritic cells during differentiation and regulates their expansion." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 3, pp. 1399-405, 2005
Shadduck, Fischer, DePasquale, Gilmore: "Paradoxical stimulation of normal and leukemic rat hematopoiesis by monoclonal antibody to CSF-1 receptor." in: Experimental hematology, Vol. 24, Issue 2, pp. 314-7, 1996
Gilmore, Shadduck: "Inhibition of day-12 spleen colony-forming units by a monoclonal antibody to the murine macrophage/monocyte colony-stimulating factor receptor." in: Blood, Vol. 85, Issue 10, pp. 2731-4, 1995