anti-Human EGF antibody for Western Blotting

Recommended EGF Antibody (supplied by: Log in to see )

Antigen
Epidermal Growth Factor (EGF) Antibodies
  • HOMG4
  • URG
  • AI790464
  • CEGF
  • epidermal growth factor
  • pro-epidermal growth factor
  • EGF
  • egf
  • CpipJ_CPIJ020278
  • Egf
Epitope
Middle Region
25
18
17
16
12
12
12
11
10
9
8
8
7
5
5
5
5
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
381
185
108
19
16
9
4
2
1
1
1
Host
Rabbit
369
148
32
26
6
3
Clonality
Polyclonal
Conjugate
This EGF antibody is un-conjugated
57
29
23
11
11
9
6
6
4
4
4
4
4
4
4
4
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
417
282
159
101
54
33
30
30
27
27
23
16
14
7
7
5
4
3
2
1
1
Options
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Catalog No. ABIN2776844
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1555064 ABIN1449300 FACS IF IP WB Mouse IgG2b AA 648-1186 Log in to see 6F1 9
3.1555064 ABIN656805 IHC (p) WB Rabbit Ig Fraction AA 690-720, Center Log in to see Polyclonal 1
3.1555064 ABIN2869485 ELISA ICC WB Mouse IgG1 Log in to see 4E11 4
3.1555064 ABIN2869465 ELISA IHC WB Mouse IgG1 Log in to see 9D7F11 4
3.1555064 ABIN3222771 IHC WB Rabbit Log in to see Polyclonal 0
3.1555064 ABIN2801138 WB Rabbit Log in to see Polyclonal 0
3.1555064 ABIN952036 EIA IHC (p) WB Rabbit Ig Fraction AA 689-720, Middle Region Log in to see Polyclonal 3
3.1555064 ABIN446990 IHC IHC (p) WB Rabbit Internal Region Log in to see Polyclonal 3
3.1555064 ABIN3184415 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal 0
3.1555064 ABIN3044516 ELISA WB Rabbit IgG AA 971-1023 Log in to see Polyclonal 0
3.1555064 ABIN1843510 IHC ELISA WB Mouse IgG1 Log in to see 0
3.1555064 ABIN1843511 ELISA IF/ICC WB Mouse IgG1 Log in to see 0
3.1555064 ABIN452469 ELISA (Detection) IF WB Mouse IgG1 Log in to see S-146 0
3.1555064 ABIN6261457 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
3.1555064 ABIN6261458 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
3.1555064 ABIN969091 IHC ELISA WB Mouse IgG1 Log in to see 9D7F11 3
3.1555064 ABIN1872450 IHC WB Rabbit IgG Log in to see Polyclonal 0
3.1555064 ABIN1537657 WB Rabbit Ig Fraction AA 940-967, C-Term Log in to see Polyclonal 0
3.1555064 ABIN6257599 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 1
3.1555064 ABIN1858692 ICC IHC WB Rabbit IgG AA 971-1023 Log in to see Polyclonal 0

General

Antigen Epidermal Growth Factor (EGF) Antibodies
Epitope Middle Region
(25), (18), (17), (16), (12), (12), (12), (11), (10), (9), (8), (8), (7), (5), (5), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(381), (185), (108), (19), (16), (9), (4), (2), (1), (1), (1)
Host Rabbit
(369), (148), (32), (26), (6), (3)
Clonality
Polyclonal
Conjugate This EGF antibody is un-conjugated
(57), (29), (23), (11), (11), (9), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(417), (282), (159), (101), (54), (33), (30), (30), (27), (27), (23), (16), (14), (7), (7), (5), (4), (3), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-EGF Antibody

Target Details EGF Application Details Handling References for anti-EGF antibody (ABIN2776844) Images
Blocking Peptide Blocking peptide for this product available: ABIN976172
Sequence ITIDFLTDKL YWCDAKQSVI EMANLDGSKR RRLTQNDVGH PFAVAVFEDY
Predicted Reactivity Dog: 86%, Horse: 93%, Human: 100%, Mouse: 79%, Pig: 93%, Rabbit: 93%, Rat: 86%
Characteristics This is a rabbit polyclonal antibody against EGF. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human EGF
Plasmids, Primers & others

Target Details EGF

Product Details anti-EGF Antibody Application Details Handling References for anti-EGF antibody (ABIN2776844) Images back to top
Antigen
Alternative Name EGF (EGF Antibody Abstract)
Background Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-
Alias Symbols: URG, HOMG4
Protein Interaction Partner: EGFR, PCGF2, UBE2I, SREBF1, LMNB1, EMD, LMNA, ZFYVE28, PIK3R2, ADAM12, DUSP3, VTN, ERBB3, ERBB2, NRG1, EGF, CPM, ADRBK1, GRB2, MAPKAPK2, RPS6KA5, FNBP1,
Protein Size: 1207
Molecular Weight 6 kDa
Gene ID 1950
NCBI Accession NM_001963, NP_001954
UniProt P01133
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

Application Details

Product Details anti-EGF Antibody Target Details EGF Handling References for anti-EGF antibody (ABIN2776844) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 1207 AA

Restrictions For Research Use only

Handling

Product Details anti-EGF Antibody Target Details EGF Application Details References for anti-EGF antibody (ABIN2776844) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-EGF antibody (ABIN2776844)

Product Details anti-EGF Antibody Target Details EGF Application Details Handling Images back to top
Product cited in:

Teixeira, Ribeiro, Cardoso, Pinto, Lobo, Fraga, Pina, Calais-da-Silva, Medeiros: "Genetic polymorphism in EGF is associated with prostate cancer aggressiveness and progression-free interval in androgen blockade-treated patients." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 11, pp. 3367-71, 2008

Images

Product Details anti-EGF Antibody Target Details EGF Application Details Handling References for anti-EGF antibody (ABIN2776844) back to top
Supplier Images
Western Blotting (WB) image for anti-Epidermal Growth Factor (EGF) (Middle Region) antibody (ABIN2776844) WB Suggested Anti-EGF Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:1562500 Posi...