anti-Bat EPH Receptor A4 antibody for Immunohistochemistry

Recommended EPH Receptor A4 Antibody (supplied by: Log in to see )

Antigen
EPH Receptor A4 (EPHA4) Antibodies
  • HEK8
  • SEK
  • TYRO1
  • RGD1560587
  • ephe
  • EPHA4
  • Ci-Eph1
  • sek
  • hek8
  • rtk1
  • tyr01
  • 2900005C20Rik
  • AI385584
  • Cek8
  • Hek8
  • Sek
  • Sek1
  • Tyro1
  • rb
  • XEpha4
  • epha4
  • pag
  • sek-1
  • EphA4
  • apha3
  • ek2
  • epha3
  • sb:cb246
  • zek2
  • zgc:109882
  • EPH receptor A4
  • Eph receptor A4
  • ephrin receptor epsilon
  • EPH receptor A4 L homeolog
  • eph receptor A4a
  • EPHA4
  • Epha4
  • ephe
  • epha4
  • epha4.L
  • epha4a
Epitope
Internal Region
21
19
18
15
14
12
11
11
11
7
6
5
4
4
4
2
2
2
1
1
1
1
1
Reactivity
Bat, Cow (Bovine), Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
174
85
40
9
9
8
6
6
6
5
3
3
3
2
2
2
2
1
1
Host
Rabbit
169
21
18
1
Clonality
Polyclonal
Conjugate
This EPH Receptor A4 antibody is un-conjugated
10
9
9
6
6
6
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
153
100
97
52
20
18
13
7
6
5
2
2
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Available images

Immunohistochemistry (IHC) image for anti-EPH Receptor A4 (EPHA4) (Internal Region) antibody (ABIN1048570)
Immunohistochemistry (IHC) image for anti-EPH Receptor A4 (EPHA4) (Internal Region) antibody (ABIN1048570)
Catalog No. ABIN1048570
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.863943 ABIN1048571 IHC IHC (p) Rabbit Internal Region Log in to see Polyclonal 0
9.863943 ABIN213398 ELISA IHC IHC (p) Rabbit C-Term Log in to see Polyclonal 0
6.8639426 ABIN2469317 IHC ELISA Rabbit C-Term Log in to see Polyclonal 0

General

Antigen EPH Receptor A4 (EPHA4) Antibodies
Epitope Internal Region
(21), (19), (18), (15), (14), (12), (11), (11), (11), (7), (6), (5), (4), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Bat, Cow (Bovine), Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(174), (85), (40), (9), (9), (8), (6), (6), (6), (5), (3), (3), (3), (2), (2), (2), (2), (1), (1)
Host Rabbit
(169), (21), (18), (1)
Clonality
Polyclonal
Conjugate This EPH Receptor A4 antibody is un-conjugated
(10), (9), (9), (6), (6), (6), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(153), (100), (97), (52), (20), (18), (13), (7), (6), (5), (2), (2)
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Product Details anti-EPH Receptor A4 Antibody

Target Details EPH Receptor A4 Application Details Handling Images
Brand IHC-plus™
Specificity Human EPHA4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig (100%) Opossum, Platypus (88%).
Purification Immunoaffinity purified
Immunogen Synthetic 16 amino acid peptide from internal region of human EPHA4. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig (100%), Opossum, Platypus (88%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Target Details EPH Receptor A4

Product Details anti-EPH Receptor A4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name EPHA4 / EPH Receptor A4 (EPHA4 Antibody Abstract)
Background Name/Gene ID: EPHA4
Subfamily: Ephrin Receptor
Family: Protein Kinase

Synonyms: EPHA4, CEK8, EK8, Ephrin type-A receptor 4, HEK8, SEK, TYRO1, TYRO1 protein tyrosine kinase, Tyrosine-protein kinase TYRO1, EPH receptor A4, EPH-like kinase 8
Gene ID 2043
Pathways RTK Signaling

Application Details

Product Details anti-EPH Receptor A4 Antibody Target Details EPH Receptor A4 Handling Images back to top
Application Notes Approved: IHC, IHC-P (6 μg/mL)
Comment

Target Species of Antibody: Human
Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

Product Details anti-EPH Receptor A4 Antibody Target Details EPH Receptor A4 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-EPH Receptor A4 Antibody Target Details EPH Receptor A4 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-EPH Receptor A4 (EPHA4) (Internal Region) antibody (ABIN1048570) Anti-EPHA4 antibody ABIN1048570 IHC staining of human kidney, glomerulus. Immunohist...
Immunohistochemistry (IHC) image for anti-EPH Receptor A4 (EPHA4) (Internal Region) antibody (ABIN1048570) Anti-EPHA4 antibody IHC of human kidney, glomerulus. Immunohistochemistry of formalin...