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Submit your validation data for this product and get a full refund. I want to validate this productRelevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
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9.863943 | ABIN1048571 | IHC IHC (p) | Rabbit | Internal Region | Log in to see | Polyclonal | 0 | |||
9.863943 | ABIN213398 | ELISA IHC IHC (p) | Rabbit | C-Term | Log in to see | Polyclonal | 0 | |||
6.8639426 | ABIN2469317 | IHC ELISA | Rabbit | C-Term | Log in to see | Polyclonal | 0 |
General |
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Antigen | EPH Receptor A4 (EPHA4) Antibodies |
Epitope | Internal Region Alternatives |
Reactivity | Bat, Cow (Bovine), Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus) Alternatives |
Host | Rabbit Alternatives |
Clonality | |
Conjugate | This EPH Receptor A4 antibody is un-conjugated Alternatives |
Application |
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Alternatives
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Supplier | Log in to see |
Product Details anti-EPH Receptor A4 AntibodyTarget Details EPH Receptor A4 Application Details Handling Images |
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Brand | IHC-plus™ |
Specificity | Human EPHA4. BLAST analysis of the peptide immunogen showed no homology with other human proteins. |
Predicted Reactivity | Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig (100%) Opossum, Platypus (88%). |
Purification | Immunoaffinity purified |
Immunogen |
Synthetic 16 amino acid peptide from internal region of human EPHA4. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig (100%), Opossum, Platypus (88%). Type of Immunogen: Synthetic peptide |
Plasmids, Primers & others | |
Target Details EPH Receptor A4Product Details anti-EPH Receptor A4 Antibody Application Details Handling Images back to top |
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Antigen | |
Alternative Name | EPHA4 / EPH Receptor A4 (EPHA4 Antibody Abstract) |
Background |
Name/Gene ID: EPHA4 Subfamily: Ephrin Receptor Family: Protein Kinase Synonyms: EPHA4, CEK8, EK8, Ephrin type-A receptor 4, HEK8, SEK, TYRO1, TYRO1 protein tyrosine kinase, Tyrosine-protein kinase TYRO1, EPH receptor A4, EPH-like kinase 8 |
Gene ID | 2043 |
Pathways | RTK Signaling |
Application DetailsProduct Details anti-EPH Receptor A4 Antibody Target Details EPH Receptor A4 Handling Images back to top |
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Application Notes | Approved: IHC, IHC-P (6 μg/mL) |
Comment |
Target Species of Antibody: Human |
Assay Procedure |
The IHC-pro Immunohistochemistry Protocol Tissue Preparation Formalin fixation and embedding in paraffin wax Tissue Sectioning Make 4-µm sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-drying oven for 45 minutes at 60°C Deparaffinization Wash slides in 3 changes of xylene – 5 minutes each at room temperature. Rehydration Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature. Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature. Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature. Rinse slides in gentle running distilled water – 5 minutes at room temperature. Antigen retrieval Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes Remove from heat and let stand at room temperature in buffer - 20 minutes Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature. Immunostaining Do not allow tissues to dry at any time during the staining procedure. Apply a universal protein block – 20 minutes at room temperature. Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature. Rinse slides in 1X TBST - 1 minute at room temperature. Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature. Rinse slides 1X TBST – 1 minute at room temperature. Apply alkaline phosphatase streptavidin – 30 minutes at room temperature. Rinse slides in 1X TBST - 1 minute at room temperature. Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature. Wash slides in distilled water – 1 minute at room temperature. Dehydrate This method should only be used if the chromogen substrate is alcohol insoluble. Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature. Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature. Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature. Wash slides in 3 changes of xylene – 1 minute each at room temperature. Apply coverslip |
Restrictions | For Research Use only |
HandlingProduct Details anti-EPH Receptor A4 Antibody Target Details EPH Receptor A4 Application Details Images back to top |
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Format | Liquid |
Concentration | Lot specific |
Buffer | PBS, less than 0.1 % sodium azide. |
Preservative | Sodium azide |
Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Storage | 4 °C,-20 °C |
Storage Comment | Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles. |
Expiry Date | 12 months |
ImagesProduct Details anti-EPH Receptor A4 Antibody Target Details EPH Receptor A4 Application Details Handling back to top |
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Supplier Images |