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|Antigen||EPH Receptor A4 (EPHA4) Antibodies|
|Conjugate||This EPH Receptor A4 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
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Product Details anti-EPH Receptor A4 AntibodyTarget Details EPH Receptor A4 Application Details Handling References for anti-EPH Receptor A4 antibody (ABIN359795) Images
|Specificity||This antibody reacts to EphA4.|
|Purification||Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human EphA4.|
Target Details EPH Receptor A4Product Details anti-EPH Receptor A4 Antibody Application Details Handling References for anti-EPH Receptor A4 antibody (ABIN359795) Images back to top
|Alternative Name||EPHA4 (EPHA4 Antibody Abstract)|
|Background||Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).Synonyms: Ephrin type-A receptor 4, HEK8, Receptor protein-tyrosine kinase HEK8, SEK, TYRO1, Tyrosine-protein kinase TYRO1, Tyrosine-protein kinase receptor SEK|
|Gene ID||2043, 9606|
Application DetailsProduct Details anti-EPH Receptor A4 Antibody Target Details EPH Receptor A4 Handling References for anti-EPH Receptor A4 antibody (ABIN359795) Images back to top
ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-EPH Receptor A4 Antibody Target Details EPH Receptor A4 Application Details References for anti-EPH Receptor A4 antibody (ABIN359795) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-EPH Receptor A4 antibody (ABIN359795)Product Details anti-EPH Receptor A4 Antibody Target Details EPH Receptor A4 Application Details Handling Images back to top
Prevost, Woulfe, Tanaka, Brass: "Interactions between Eph kinases and ephrins provide a mechanism to support platelet aggregation once cell-to-cell contact has occurred." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 14, pp. 9219-24, 2002
Xu, Mellitzer, Wilkinson: "Roles of Eph receptors and ephrins in segmental patterning." in: Philosophical transactions of the Royal Society of London. Series B, Biological sciences, Vol. 355, Issue 1399, pp. 993-1002, 2000
Jordan, Solan, Dominguez, Sia, Hand, Lampe, Laird: "Trafficking, assembly, and function of a connexin43-green fluorescent protein chimera in live mammalian cells." in: Molecular biology of the cell, Vol. 10, Issue 6, pp. 2033-50, 1999
Zhou: "The Eph family receptors and ligands." in: Pharmacology & therapeutics, Vol. 77, Issue 3, pp. 151-81, 1998
Fox, Holst, Chute, Lindberg, Janssen, Basu, Welcher: "cDNA cloning and tissue distribution of five human EPH-like receptor protein-tyrosine kinases." in: Oncogene, Vol. 10, Issue 5, pp. 897-905, 1995
ImagesProduct Details anti-EPH Receptor A4 Antibody Target Details EPH Receptor A4 Application Details Handling References for anti-EPH Receptor A4 antibody (ABIN359795) back to top