anti-Human EPH Receptor B2 antibody for Western Blotting

Recommended EPH Receptor B2 Antibody (supplied by: Log in to see )

Antigen
EPH Receptor B2 (EPHB2) Antibodies
  • CAPB
  • DRT
  • EK5
  • EPHT3
  • ERK
  • Hek5
  • PCBC
  • Tyro5
  • Cek5
  • Drt
  • ETECK
  • Erk
  • Nuk
  • Prkm5
  • Qek5
  • Sek3
  • RGD1564232
  • EPH receptor B2
  • Eph receptor B2
  • EPHB2
  • Ephb2
Epitope
AA 27-548
26
14
12
12
11
11
11
8
7
7
4
4
3
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
168
57
6
4
3
3
3
3
2
2
1
1
1
1
Host
Goat
90
41
25
19
Clonality
Polyclonal
Conjugate
This EPH Receptor B2 antibody is un-conjugated
10
7
7
7
7
6
3
2
2
2
2
2
2
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Simple Western (SimWes), Western Blotting (WB)
127
101
62
44
31
30
13
7
3
1
1
1
1
1
Supplier
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Catalog No. ABIN4900040
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1493003 ABIN1533687 ELISA WB Rabbit IgG AA 991-1040 Log in to see Polyclonal 1
3.1493003 ABIN391920 IF IHC (p) WB Rabbit Ig Fraction AA 1021-1050, C-Term Log in to see Polyclonal 3
3.1493003 ABIN359812 EIA IHC (p) WB Rabbit Ig Fraction N-Term Log in to see Polyclonal 5
3.1493003 ABIN359813 EIA IHC (p) WB Rabbit Ig Fraction C-Term Log in to see Polyclonal 5
3.1493003 ABIN391921 FACS IHC (p) WB Rabbit Ig Fraction Log in to see Polyclonal 3
3.1493003 ABIN387812 FACS IHC (p) WB Mouse IgG1 kappa Log in to see 48CT12-6-4 5
3.1493003 ABIN359814 EIA IHC (p) WB Rabbit Ig Fraction Log in to see Polyclonal 5
3.1493003 ABIN614403 EIA IF IHC (p) WB Mouse IgG2b AA 17-200 Log in to see 2D12C6 0
3.1493003 ABIN188674 ELISA WB Goat Log in to see Polyclonal 2
3.1493003 ABIN356401 EIA IHC (p) WB Mouse IgG1 Log in to see 48CT12-6-4 0
3.1493003 ABIN2465658 ELISA WB Goat Internal Region Log in to see Polyclonal 0
3.1493003 ABIN3184511 ELISA IHC WB Rabbit IgG C-Term Log in to see Polyclonal 0
3.1493003 ABIN1724695 ICC ELISA WB Mouse IgG2b AA 17-200 Log in to see 2D12C6 2
3.1493003 ABIN1843505 ELISA IF/ICC WB Mouse IgG2b AA 17-200 Log in to see 0
3.1493003 ABIN4308695 ELISA ICC IF WB Mouse IgG2b Log in to see 2D12C6 0
3.1493003 ABIN515343 ELISA WB Mouse IgG2a kappa AA 226-325, partial Log in to see 4D1 0
3.1493003 ABIN4903589 WB Rabbit IgG Log in to see Polyclonal 0
3.1493003 ABIN2562445 ELISA WB Goat IgG Internal Region Log in to see Polyclonal 0
3.1493003 ABIN4308694 ELISA IHC IHC (p) WB Rabbit Log in to see Polyclonal 0
3.1493003 ABIN3030824 FACS IHC ELISA WB Mouse IgG1, kappa Log in to see 48CT12-6-4 0

General

Antigen EPH Receptor B2 (EPHB2) Antibodies
Epitope AA 27-548
(26), (14), (12), (12), (11), (11), (11), (8), (7), (7), (4), (4), (3), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(168), (57), (6), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Host Goat
(90), (41), (25), (19)
Clonality
Polyclonal
Conjugate This EPH Receptor B2 antibody is un-conjugated
(10), (7), (7), (7), (7), (6), (3), (2), (2), (2), (2), (2), (2), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Simple Western (SimWes), Western Blotting (WB)
(127), (101), (62), (44), (31), (30), (13), (7), (3), (1), (1), (1), (1), (1)
Pubmed 23 references available
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Product Details anti-EPH Receptor B2 Antibody

Target Details EPH Receptor B2 Application Details Handling References for anti-EPH Receptor B2 antibody (ABIN4900040) Images
Specificity Detects mouse and human EphB2 in direct ELISAs and Western blots. In Western blots, approximately 5 % cross-reactivity with recombinant rat (rr) EphB1, recombinant mouse (rm) EphA8, rmEphA6, rmEphB6, rmEphA3, rmEphA4, rmEphA7, rrEphA5, recombinant human EphA1, rmEphA2 and rmEphB3 is observed.
Purification Antigen Affinity-purified
Sterility 0.2 μm filtered
Immunogen Mouse myeloma cell line NS0-derived recombinant mouse EphB2, Val27-Lys548, Accession # P54763
Isotype IgG
Plasmids, Primers & others

Target Details EPH Receptor B2

Product Details anti-EPH Receptor B2 Antibody Application Details Handling References for anti-EPH Receptor B2 antibody (ABIN4900040) Images back to top
Antigen
Alternative Name EphB2 (EPHB2 Antibody Abstract)
Background Alternative Names: CAPB, Cek5, Drt, DRTEphB2, EC 2.7.10, EC 2.7.10.1, EK5, elk-related tyrosine kinase, EPH receptor B2, eph tyrosine kinase 3, EPH-like kinase 5, ephrin type-B receptor 2, EPHT3MGC87492, EPTH3, Erk, ERKHek5, HEK5, Nuk, PCBC, protein-tyrosine kinase HEK5, Qek2, Renal carcinoma antigen NY-REN-47, Sek3, Tyro5, Tyrosine-protein kinase receptor EPH-3, Tyrosine-protein kinase TYRO5
Gene ID 2048
UniProt P54763
Pathways RTK Signaling, Regulation of long-term Neuronal Synaptic Plasticity

Application Details

Product Details anti-EPH Receptor B2 Antibody Target Details EPH Receptor B2 Handling References for anti-EPH Receptor B2 antibody (ABIN4900040) Images back to top
Application Notes Western Blot 0.1 μg/mL
Simple Western 20 μg/mL
Flow Cytometry 0.25 μg/10^6 cells
Immunohistochemistry 1-15 μg/mL
Immunocytochemistry 5-15 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-EPH Receptor B2 Antibody Target Details EPH Receptor B2 Application Details References for anti-EPH Receptor B2 antibody (ABIN4900040) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-EPH Receptor B2 antibody (ABIN4900040)

Product Details anti-EPH Receptor B2 Antibody Target Details EPH Receptor B2 Application Details Handling Images back to top
Product cited in:

Tüysüz, van Bloois, van den Brink, Begthel, Verstegen, Cruz, Hui, van der Laan, de Jonge, Vries, Braakman, Mastrobattista, Cornelissen, Clevers, Ten Berge: "Lipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells." in: Nature communications, Vol. 8, pp. 14578, 2017 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).

Liu, Tsai, Chen, Yang, Chang, Cheng, Turner, Yanagawa, Wang, Yu: "Ascl1 promotes tangential migration and confines migratory routes by induction of Ephb2 in the telencephalon." in: Scientific reports, Vol. 7, pp. 42895, 2017 Method employed by authors: Immunocytochemistry (ICC) (Sample species: Mouse (Murine)).

Miura, Suzuki: "Generation of Mouse and Human Organoid-Forming Intestinal Progenitor Cells by Direct Lineage Reprogramming." in: Cell stem cell, Vol. 21, Issue 4, pp. 456-471.e5, 2017 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).

van Lidth de Jeude, Meijer, Wielenga, Spaan, Baan, Rosekrans, Meisner, Shen, Lee, Paton, Paton, Muncan, van den Brink, Heijmans: "Induction of endoplasmic reticulum stress by deletion of Grp78 depletes Apc mutant intestinal epithelial stem cells." in: Oncogene, 2016 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)).

Cissé, Duplan, Lorivel, Dunys, Bauer, Meckler, Gerakis, Lauritzen, Checler: "The transcription factor XBP1s restores hippocampal synaptic plasticity and memory by control of the Kalirin-7 pathway in Alzheimer model." in: Molecular psychiatry, 2016 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Mouse (Murine)).

Depner, Zum Buttel, Böğürcü, Cuesta, Aburto, Seidel, Finkelmeier, Foss, Hofmann, Kaulich, Barbus, Segarra, Reifenberger, Garvalov, Acker, Acker-Palmer: "EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance." in: Nature communications, Vol. 7, pp. 12329, 2016 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)).

Feng, Sakamoto, Wu, Liu, Wiese, Green, Green, Akyol, Roy, Zhai, Cho, Fearon: "Tissue-Specific Effects of Reduced β-catenin Expression on Adenomatous Polyposis Coli Mutation-Instigated Tumorigenesis in Mouse Colon and Ovarian Epithelium." in: PLoS genetics, Vol. 11, Issue 11, pp. e1005638, 2015 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)).

Dong, Gao, Wang, Miao, Wang, Wu, Li, Wu, Cheng, Sun, Yang, Wang: "GluA2 trafficking is involved in apoptosis of retinal ganglion cells induced by activation of EphB/EphrinB reverse signaling in a rat chronic ocular hypertension model." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 13, pp. 5409-21, 2015 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Rat (Rattus)).

Boyer-Di Ponio, El-Ayoubi, Glacial, Ganeshamoorthy, Driancourt, Godet, Perrière, Guillevic, Couraud, Uzan: "Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes." in: PLoS ONE, Vol. 9, Issue 1, pp. e84179, 2014 Method employed by authors: Immunocytochemistry (ICC), Immunocytochemistry (ICC) (Sample species: Human).

Aharon, Janes, Burgess, Hamza, Klebaner, Lackmann: "A mathematical model for eph/ephrin-directed segregation of intermingled cells." in: PLoS ONE, Vol. 9, Issue 12, pp. e111803, 2014 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Bouché, Romero-Ortega, Henkemeyer, Catchpole, Leemhuis, Frotscher, May, Herz, Bock: "Reelin induces EphB activation." in: Cell research, Vol. 23, Issue 4, pp. 473-90, 2013

Attwood, Bourgognon, Patel, Mucha, Schiavon, Skrzypiec, Young, Shiosaka, Korostynski, Piechota, Przewlocki, Pawlak: "Neuropsin cleaves EphB2 in the amygdala to control anxiety." in: Nature, Vol. 473, Issue 7347, pp. 372-5, 2011 Method employed by authors: In vivo Studies (in vivo), In vivo Studies (in vivo) (Sample species: Mouse (Murine)).

Furukawa, Sato, Katsuno, Nakagawa, Noguchi, Tokumasa, Yokote, Yokosuka, Saito: "Smad3 contributes to positioning of proliferating cells in colonic crypts by inducing EphB receptor protein expression." in: Biochemical and biophysical research communications, Vol. 405, Issue 4, pp. 521-6, 2011 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)).

Bush, Soriano: "Ephrin-B1 regulates axon guidance by reverse signaling through a PDZ-dependent mechanism." in: Genes & development, Vol. 23, Issue 13, pp. 1586-99, 2009 Method employed by authors: Immunoprecipitation (IP), Immunoprecipitation (IP) (Sample species: Mouse (Murine)).

Lim, Matsuda, Poo: "Ephrin-B reverse signaling promotes structural and functional synaptic maturation in vivo." in: Nature neuroscience, Vol. 11, Issue 2, pp. 160-9, 2008 Method employed by authors: Immunoprecipitation (IP), Immunoprecipitation (IP) (Sample species: Xenopus laevis).

Allan, Häusler, Wei, Gooi, Quinn, Crimeen-Irwin, Pompolo, Sims, Gillespie, Onyia, Martin: "EphrinB2 regulation by PTH and PTHrP revealed by molecular profiling in differentiating osteoblasts." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 23, Issue 8, pp. 1170-81, 2008 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)).

Saraswathi, Gao, Morrow, Chait, Niswender, Hasty: "Fish oil increases cholesterol storage in white adipose tissue with concomitant decreases in inflammation, hepatic steatosis, and atherosclerosis in mice." in: The Journal of nutrition, Vol. 137, Issue 7, pp. 1776-82, 2007 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Chaudhari, Mahfouz, Fialho, Yamada, Granja, Zhu, Hashimoto, Schlarb-Ridley, Cho, Das Gupta, Chakrabarty: "Cupredoxin-cancer interrelationship: azurin binding with EphB2, interference in EphB2 tyrosine phosphorylation, and inhibition of cancer growth." in: Biochemistry, Vol. 46, Issue 7, pp. 1799-810, 2007 Method employed by authors: Immunoprecipitation (IP), Immunoprecipitation (IP) (Sample species: Human).

Tanaka, Kamata, Takigahira, Yanagihara, Sakai: "Phosphorylation of ephrin-B1 regulates dissemination of gastric scirrhous carcinoma." in: The American journal of pathology, Vol. 171, Issue 1, pp. 68-78, 2007 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)).

Ogawa, Wada, Okada, Harada, Nakajima, Pasquale, Tsuyama: "EphB2 and ephrin-B1 expressed in the adult kidney regulate the cytoarchitecture of medullary tubule cells through Rho family GTPases." in: Journal of cell science, Vol. 119, Issue Pt 3, pp. 559-70, 2006 Method employed by authors: Immunohistochemistry (IHC), Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).

Images

Product Details anti-EPH Receptor B2 Antibody Target Details EPH Receptor B2 Application Details Handling References for anti-EPH Receptor B2 antibody (ABIN4900040) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-EPH Receptor B2 (EPHB2) (AA 27-548) antibody (ABIN4900040) Detection of EphB2 in COLO 205 Human Cell Line by Flow Cytometry. COLO 205 human colo...
Flow Cytometry (FACS) image for anti-EPH Receptor B2 (EPHB2) (AA 27-548) antibody (ABIN4900040) Detection of EphB2 in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem c...
Immunohistochemistry (IHC) image for anti-EPH Receptor B2 (EPHB2) (AA 27-548) antibody (ABIN4900040) EphB2 in Human Esophageal Squamous Cell Carcinoma. EphB2 was detected in immersion fi...
Immunohistochemistry (IHC) image for anti-EPH Receptor B2 (EPHB2) (AA 27-548) antibody (ABIN4900040) EphB2 in Mouse Embryo. EphB2 was detected in immersion fixed frozen sections of mouse...
Immunocytochemistry (ICC) image for anti-EPH Receptor B2 (EPHB2) (AA 27-548) antibody (ABIN4900040) EphB2 in MBA-MB-468 Human Cell Line. EphB2 was detected in immersion fixed MBA-MB-468...
Immunohistochemistry (IHC) image for anti-EPH Receptor B2 (EPHB2) (AA 27-548) antibody (ABIN4900040) EphB2 in Embryonic Mouse Brain. EphB2 was detected in immersion fixed frozen sections...
Simple Western (SimWes) image for anti-EPH Receptor B2 (EPHB2) (AA 27-548) antibody (ABIN4900040) Detection of Human EphB2 by Simple WesternTM. Simple Western lane view shows lysates ...
Western Blotting (WB) image for anti-EPH Receptor B2 (EPHB2) (AA 27-548) antibody (ABIN4900040) Detection of Recombinant Human and Mouse EphB2 by Western Blot. Western blot shows 25...