anti-Mouse (Murine) EPH Receptor B2 antibody for Immunocytochemistry

Recommended EPH Receptor B2 Antibody (supplied by: Log in to see )

Antigen
EPH Receptor B2 (EPHB2) Antibodies
  • CAPB
  • DRT
  • EK5
  • EPHT3
  • ERK
  • Hek5
  • PCBC
  • Tyro5
  • Cek5
  • Drt
  • ETECK
  • Erk
  • Nuk
  • Prkm5
  • Qek5
  • Sek3
  • RGD1564232
  • EPH receptor B2
  • Eph receptor B2
  • EPHB2
  • Ephb2
Epitope
AA 27-548
25
14
12
12
12
11
8
7
7
5
4
4
3
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
159
50
6
6
4
3
3
3
2
2
1
1
1
1
Host
Goat
92
40
19
18
Clonality
Polyclonal
Conjugate
This EPH Receptor B2 antibody is un-conjugated
10
7
7
7
7
6
2
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Simple Western (SimWes), Western Blotting (WB)
127
99
60
37
31
30
14
7
3
1
1
1
1
1
Supplier
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Catalog No. ABIN4900040
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
25.686504 ABIN4900039 CyTOF FACS ICC Rat IgG2a AA 27-548 Log in to see 512012 1
1 ABIN603140 ICC FACS ELISA Rat IgG2a AA 27-551 Log in to see 0
1 ABIN1831710 ICC FACS ELISA WB Goat IgG Log in to see Polyclonal 0

General

Antigen EPH Receptor B2 (EPHB2) Antibodies
Epitope AA 27-548
(25), (14), (12), (12), (12), (11), (8), (7), (7), (5), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(159), (50), (6), (6), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Host Goat
(92), (40), (19), (18)
Clonality
Polyclonal
Conjugate This EPH Receptor B2 antibody is un-conjugated
(10), (7), (7), (7), (7), (6), (2), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Simple Western (SimWes), Western Blotting (WB)
(127), (99), (60), (37), (31), (30), (14), (7), (3), (1), (1), (1), (1), (1)
Pubmed 26 references available
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Product Details anti-EPH Receptor B2 Antibody

Target Details EPH Receptor B2 Application Details Handling References for anti-EPH Receptor B2 antibody (ABIN4900040) Images
Specificity Detects mouse and human EphB2 in direct ELISAs and Western blots. In Western blots, approximately 5 % cross-reactivity with recombinant rat (rr) EphB1, recombinant mouse (rm) EphA8, rmEphA6, rmEphB6, rmEphA3, rmEphA4, rmEphA7, rrEphA5, recombinant human EphA1, rmEphA2 and rmEphB3 is observed.
Purification Antigen Affinity-purified
Sterility 0.2 μm filtered
Immunogen Mouse myeloma cell line NS0-derived recombinant mouse EphB2, Val27-Lys548, Accession # P54763
Isotype IgG
Plasmids, Primers & others

Target Details EPH Receptor B2

Product Details anti-EPH Receptor B2 Antibody Application Details Handling References for anti-EPH Receptor B2 antibody (ABIN4900040) Images back to top
Antigen
Alternative Name EphB2 (EPHB2 Antibody Abstract)
Background Alternative Names: CAPB, Cek5, Drt, DRTEphB2, EC 2.7.10, EC 2.7.10.1, EK5, elk-related tyrosine kinase, EPH receptor B2, eph tyrosine kinase 3, EPH-like kinase 5, ephrin type-B receptor 2, EPHT3MGC87492, EPTH3, Erk, ERKHek5, HEK5, Nuk, PCBC, protein-tyrosine kinase HEK5, Qek2, Renal carcinoma antigen NY-REN-47, Sek3, Tyro5, Tyrosine-protein kinase receptor EPH-3, Tyrosine-protein kinase TYRO5
Gene ID 2048
UniProt P54763
Pathways RTK Signaling, Regulation of long-term Neuronal Synaptic Plasticity

Application Details

Product Details anti-EPH Receptor B2 Antibody Target Details EPH Receptor B2 Handling References for anti-EPH Receptor B2 antibody (ABIN4900040) Images back to top
Application Notes Western Blot 0.1 μg/mL
Simple Western 20 μg/mL
Flow Cytometry 0.25 μg/10^6 cells
Immunohistochemistry 1-15 μg/mL
Immunocytochemistry 5-15 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-EPH Receptor B2 Antibody Target Details EPH Receptor B2 Application Details References for anti-EPH Receptor B2 antibody (ABIN4900040) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-EPH Receptor B2 antibody (ABIN4900040)

Product Details anti-EPH Receptor B2 Antibody Target Details EPH Receptor B2 Application Details Handling Images back to top
Product cited in:

Mimche, Lee, Mimche, Thapa, Grakoui, Henkemeyer, Lamb: "EphB2 receptor tyrosine kinase promotes hepatic fibrogenesis in mice via activation of hepatic stellate cells." in: Scientific reports, Vol. 8, Issue 1, pp. 2532, 2018 Method employed by authors: Immunocytochemistry (ICC) (Sample species: Mouse (Murine)).

Tüysüz, van Bloois, van den Brink, Begthel, Verstegen, Cruz, Hui, van der Laan, de Jonge, Vries, Braakman, Mastrobattista, Cornelissen, Clevers, Ten Berge: "Lipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells." in: Nature communications, Vol. 8, pp. 14578, 2017 Method employed by authors: Immunohistochemistry (IHC), Immunohistochemistry (IHC) (Sample species: Human).

Liu, Tsai, Chen, Yang, Chang, Cheng, Turner, Yanagawa, Wang, Yu: "Ascl1 promotes tangential migration and confines migratory routes by induction of Ephb2 in the telencephalon." in: Scientific reports, Vol. 7, pp. 42895, 2017 Method employed by authors: Immunocytochemistry (ICC), Immunocytochemistry (ICC) (Sample species: Mouse (Murine)).

Miura, Suzuki: "Generation of Mouse and Human Organoid-Forming Intestinal Progenitor Cells by Direct Lineage Reprogramming." in: Cell stem cell, Vol. 21, Issue 4, pp. 456-471.e5, 2017 Method employed by authors: Immunohistochemistry (IHC), Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).

ONeill, Kindberg, Niethamer, Larson, Ho, Greenberg, Bush: "Unidirectional Eph/ephrin signaling creates a cortical actomyosin differential to drive cell segregation." in: The Journal of cell biology, Vol. 215, Issue 2, pp. 217-229, 2017 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).

Shi, Sun, Hu, Liu, Hu, Sun, Yao, Sun, Hao, Wei, Han, Gao: "Blocking the Interaction between EphB2 and ADDLs by a Small Peptide Rescues Impaired Synaptic Plasticity and Memory Deficits in a Mouse Model of Alzheimer's Disease." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 36, Issue 47, pp. 11959-11973, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)).

van Lidth de Jeude, Meijer, Wielenga, Spaan, Baan, Rosekrans, Meisner, Shen, Lee, Paton, Paton, Muncan, van den Brink, Heijmans: "Induction of endoplasmic reticulum stress by deletion of Grp78 depletes Apc mutant intestinal epithelial stem cells." in: Oncogene, 2016 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)).

Cissé, Duplan, Lorivel, Dunys, Bauer, Meckler, Gerakis, Lauritzen, Checler: "The transcription factor XBP1s restores hippocampal synaptic plasticity and memory by control of the Kalirin-7 pathway in Alzheimer model." in: Molecular psychiatry, 2016 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Mouse (Murine)).

Depner, Zum Buttel, Böğürcü, Cuesta, Aburto, Seidel, Finkelmeier, Foss, Hofmann, Kaulich, Barbus, Segarra, Reifenberger, Garvalov, Acker, Acker-Palmer: "EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance." in: Nature communications, Vol. 7, pp. 12329, 2016 Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)).

Feng, Sakamoto, Wu, Liu, Wiese, Green, Green, Akyol, Roy, Zhai, Cho, Fearon: "Tissue-Specific Effects of Reduced β-catenin Expression on Adenomatous Polyposis Coli Mutation-Instigated Tumorigenesis in Mouse Colon and Ovarian Epithelium." in: PLoS genetics, Vol. 11, Issue 11, pp. e1005638, 2015 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)).

Dong, Gao, Wang, Miao, Wang, Wu, Li, Wu, Cheng, Sun, Yang, Wang: "GluA2 trafficking is involved in apoptosis of retinal ganglion cells induced by activation of EphB/EphrinB reverse signaling in a rat chronic ocular hypertension model." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 13, pp. 5409-21, 2015 Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Rat (Rattus)).

Boyer-Di Ponio, El-Ayoubi, Glacial, Ganeshamoorthy, Driancourt, Godet, Perrière, Guillevic, Couraud, Uzan: "Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes." in: PLoS ONE, Vol. 9, Issue 1, pp. e84179, 2014 Method employed by authors: Immunocytochemistry (ICC), Immunocytochemistry (ICC), Immunocytochemistry (ICC) (Sample species: Human).

Aharon, Janes, Burgess, Hamza, Klebaner, Lackmann: "A mathematical model for eph/ephrin-directed segregation of intermingled cells." in: PLoS ONE, Vol. 9, Issue 12, pp. e111803, 2014 Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Bouché, Romero-Ortega, Henkemeyer, Catchpole, Leemhuis, Frotscher, May, Herz, Bock: "Reelin induces EphB activation." in: Cell research, Vol. 23, Issue 4, pp. 473-90, 2013

Attwood, Bourgognon, Patel, Mucha, Schiavon, Skrzypiec, Young, Shiosaka, Korostynski, Piechota, Przewlocki, Pawlak: "Neuropsin cleaves EphB2 in the amygdala to control anxiety." in: Nature, Vol. 473, Issue 7347, pp. 372-5, 2011 Method employed by authors: In vivo Studies (in vivo), In vivo Studies (in vivo), In vivo Studies (in vivo) (Sample species: Mouse (Murine)).

Furukawa, Sato, Katsuno, Nakagawa, Noguchi, Tokumasa, Yokote, Yokosuka, Saito: "Smad3 contributes to positioning of proliferating cells in colonic crypts by inducing EphB receptor protein expression." in: Biochemical and biophysical research communications, Vol. 405, Issue 4, pp. 521-6, 2011 Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)).

Bush, Soriano: "Ephrin-B1 regulates axon guidance by reverse signaling through a PDZ-dependent mechanism." in: Genes & development, Vol. 23, Issue 13, pp. 1586-99, 2009 Method employed by authors: Immunoprecipitation (IP), Immunoprecipitation (IP), Immunoprecipitation (IP) (Sample species: Mouse (Murine)).

Lim, Matsuda, Poo: "Ephrin-B reverse signaling promotes structural and functional synaptic maturation in vivo." in: Nature neuroscience, Vol. 11, Issue 2, pp. 160-9, 2008 Method employed by authors: Immunoprecipitation (IP), Immunoprecipitation (IP), Immunoprecipitation (IP) (Sample species: Xenopus laevis).

Allan, Häusler, Wei, Gooi, Quinn, Crimeen-Irwin, Pompolo, Sims, Gillespie, Onyia, Martin: "EphrinB2 regulation by PTH and PTHrP revealed by molecular profiling in differentiating osteoblasts." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 23, Issue 8, pp. 1170-81, 2008 Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)).

Saraswathi, Gao, Morrow, Chait, Niswender, Hasty: "Fish oil increases cholesterol storage in white adipose tissue with concomitant decreases in inflammation, hepatic steatosis, and atherosclerosis in mice." in: The Journal of nutrition, Vol. 137, Issue 7, pp. 1776-82, 2007 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Images

Product Details anti-EPH Receptor B2 Antibody Target Details EPH Receptor B2 Application Details Handling References for anti-EPH Receptor B2 antibody (ABIN4900040) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-EPH Receptor B2 (EPHB2) (AA 27-548) antibody (ABIN4900040) Detection of EphB2 in COLO 205 Human Cell Line by Flow Cytometry. COLO 205 human colo...
Flow Cytometry (FACS) image for anti-EPH Receptor B2 (EPHB2) (AA 27-548) antibody (ABIN4900040) Detection of EphB2 in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem c...
Immunohistochemistry (IHC) image for anti-EPH Receptor B2 (EPHB2) (AA 27-548) antibody (ABIN4900040) EphB2 in Human Esophageal Squamous Cell Carcinoma. EphB2 was detected in immersion fi...
Immunohistochemistry (IHC) image for anti-EPH Receptor B2 (EPHB2) (AA 27-548) antibody (ABIN4900040) EphB2 in Mouse Embryo. EphB2 was detected in immersion fixed frozen sections of mouse...
Immunocytochemistry (ICC) image for anti-EPH Receptor B2 (EPHB2) (AA 27-548) antibody (ABIN4900040) EphB2 in MBA-MB-468 Human Cell Line. EphB2 was detected in immersion fixed MBA-MB-468...
Immunohistochemistry (IHC) image for anti-EPH Receptor B2 (EPHB2) (AA 27-548) antibody (ABIN4900040) EphB2 in Embryonic Mouse Brain. EphB2 was detected in immersion fixed frozen sections...
Simple Western (SimWes) image for anti-EPH Receptor B2 (EPHB2) (AA 27-548) antibody (ABIN4900040) Detection of Human EphB2 by Simple WesternTM. Simple Western lane view shows lysates ...
Western Blotting (WB) image for anti-EPH Receptor B2 (EPHB2) (AA 27-548) antibody (ABIN4900040) Detection of Recombinant Human and Mouse EphB2 by Western Blot. Western blot shows 25...