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|Antigen||V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) Antibodies|
|Conjugate||This ERBB4 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
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Product Details anti-ERBB4 AntibodyTarget Details ERBB4 Application Details Handling References for anti-ERBB4 antibody (ABIN359846) Images
|Specificity||This antibody reacts to ErbB4.|
|Purification||Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ErbB4.|
Target Details ERBB4Product Details anti-ERBB4 Antibody Application Details Handling References for anti-ERBB4 antibody (ABIN359846) Images back to top
|Alternative Name||ERBB4 / HER4 (ERBB4 Antibody Abstract)|
|Background||Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.Synonyms: HER-4, Proto-oncogene-like protein c-ErbB-4, Receptor tyrosine-protein kinase erbB-4, Tyrosine kinase-type cell surface receptor HER4, c-ErbB-4, p180erbB4|
|Gene ID||2066, 9606|
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-ERBB4 Antibody Target Details ERBB4 Handling References for anti-ERBB4 antibody (ABIN359846) Images back to top
ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-ERBB4 Antibody Target Details ERBB4 Application Details References for anti-ERBB4 antibody (ABIN359846) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-ERBB4 antibody (ABIN359846)Product Details anti-ERBB4 Antibody Target Details ERBB4 Application Details Handling Images back to top
Williams, Trout, Gallo, Pitfield, Bryant, Penington, Riese: "A constitutively active ErbB4 mutant inhibits drug-resistant colony formation by the DU-145 and PC-3 human prostate tumor cell lines." in: Cancer letters, Vol. 192, Issue 1, pp. 67-74, 2003
Thomas, Chouinard, Cox, Parsons, Stallings-Mann, Garcia, Jove, Wharen: "Spontaneous activation and signaling by overexpressed epidermal growth factor receptors in glioblastoma cells." in: International journal of cancer. Journal international du cancer, Vol. 104, Issue 1, pp. 19-27, 2003
Ni, Yuan, Carpenter: "Role of the ErbB-4 carboxyl terminus in gamma-secretase cleavage." in: The Journal of biological chemistry, Vol. 278, Issue 7, pp. 4561-5, 2003
Cheng, Tikhomirov, Zhou, Carpenter: "Ectodomain cleavage of ErbB-4: characterization of the cleavage site and m80 fragment." in: The Journal of biological chemistry, Vol. 278, Issue 40, pp. 38421-7, 2003
Komuro, Nagai, Navin, Sudol: "WW domain-containing protein YAP associates with ErbB-4 and acts as a co-transcriptional activator for the carboxyl-terminal fragment of ErbB-4 that translocates to the nucleus." in: The Journal of biological chemistry, Vol. 278, Issue 35, pp. 33334-41, 2003
ImagesProduct Details anti-ERBB4 Antibody Target Details ERBB4 Application Details Handling References for anti-ERBB4 antibody (ABIN359846) back to top