anti-Pig (Porcine) ERBB4 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended ERBB4 Antibody (supplied by: Log in to see )

Antigen
V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) Antibodies
  • erbb4
  • si:ch211-227n20.1
  • Her4
  • c-erbB-4
  • HER4
  • p180erbB4
  • erb-b2 receptor tyrosine kinase 4
  • erb-b2 receptor tyrosine kinase 4a
  • v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)
  • erb-b2 receptor tyrosine kinase 4 L homeolog
  • receptor tyrosine-protein kinase ErbB-4
  • ERBB4
  • erbb4a
  • erbb4
  • erbb4.L
  • Tsp_13582
  • Erbb4
Epitope
Internal Region
42
35
35
34
20
18
17
16
15
13
12
12
9
7
6
5
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Bat, Chicken, Dog (Canine), Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus)
369
184
152
6
4
3
3
3
2
2
2
2
1
1
1
Host
Rabbit
311
66
1
Clonality
Polyclonal
Conjugate
This ERBB4 antibody is un-conjugated
20
19
17
14
13
10
7
7
6
6
6
5
5
5
5
5
5
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
216
157
126
81
68
41
33
23
19
9
4
3
2
1
1
Supplier
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Catalog No. ABIN1048578
$ 484.00
Plus shipping costs $45.00

General

Antigen V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) Antibodies
Epitope Internal Region
(42), (35), (35), (34), (20), (18), (17), (16), (15), (13), (12), (12), (9), (7), (6), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Bat, Chicken, Dog (Canine), Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(369), (184), (152), (6), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(311), (66), (1)
Clonality
Polyclonal
Conjugate This ERBB4 antibody is un-conjugated
(20), (19), (17), (14), (13), (10), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(216), (157), (126), (81), (68), (41), (33), (23), (19), (9), (4), (3), (2), (1), (1)
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Product Details anti-ERBB4 Antibody

Target Details ERBB4 Application Details Handling Images
Brand IHC-plus™
Specificity Human ERBB4. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except ERBB3 (55 % ), FRAS1 (55 %).
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gibbon, Marmoset, Mouse, Rat, Panda, Dog, Bat, Horse, Pig, Opossum, Turkey, Chicken, Platypus (100%).
Purification Immunoaffinity purified
Immunogen Synthetic 20 amino acid peptide from internal region of human ERBB4. Percent identity with other species by BLAST analysis: Human, Gibbon, Marmoset, Mouse, Rat, Panda, Dog, Bat, Horse, Pig, Opossum, Turkey, Chicken, Platypus (100%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Target Details ERBB4

Product Details anti-ERBB4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name ERBB4 / HER4 (ERBB4 Antibody Abstract)
Background Name/Gene ID: ERBB4
Subfamily: EGF Receptor
Family: Protein Kinase

Synonyms: ERBB4, ERBB 4, HER4, p180 erb4, p180erbB4
Gene ID 2066
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-ERBB4 Antibody Target Details ERBB4 Handling Images back to top
Application Notes Approved: IHC, IHC-P (16 μg/mL)
Comment

Target Species of Antibody: Human

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

Product Details anti-ERBB4 Antibody Target Details ERBB4 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-ERBB4 Antibody Target Details ERBB4 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) (Internal Region) antibody (ABIN1048578) Anti-ERBB4 antibody ABIN1048578 IHC staining of human kidney. Immunohistochemistry o...
Immunohistochemistry (IHC) image for anti-V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) (Internal Region) antibody (ABIN1048578) Anti-ERBB4 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, para...