anti-Rat (Rattus) ERBB4 antibody for Immunohistochemistry

Recommended ERBB4 Antibody (supplied by: Log in to see )

Antigen
V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) Antibodies
  • erbb4
  • si:ch211-227n20.1
  • Her4
  • c-erbB-4
  • HER4
  • p180erbB4
  • erb-b2 receptor tyrosine kinase 4
  • erb-b2 receptor tyrosine kinase 4a
  • v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)
  • erb-b2 receptor tyrosine kinase 4 L homeolog
  • receptor tyrosine-protein kinase ErbB-4
  • ERBB4
  • erbb4a
  • erbb4
  • erbb4.L
  • Tsp_13582
  • Erbb4
Epitope
pTyr1284
42
35
34
33
20
18
17
17
15
13
12
12
7
6
6
5
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
365
181
149
7
5
4
3
3
3
3
2
2
2
1
1
Host
Rabbit
310
63
1
Clonality
Polyclonal
Conjugate
This ERBB4 antibody is un-conjugated
19
19
17
14
13
10
7
7
6
6
6
5
5
5
5
5
5
2
2
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
214
156
125
81
68
38
32
20
18
9
4
3
2
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN6256632
$ 390.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
18.971891 ABIN6262216 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
18.971891 ABIN5693168 IHC WB Rabbit Log in to see Polyclonal 0
12.971891 ABIN2704882 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal 0
12.971891 ABIN2704881 IC IF IHC WB Rabbit C-Term, pTyr1284 Log in to see Polyclonal 0
12.971891 ABIN1531611 IF IHC ELISA WB Rabbit IgG AA 1250-1299, pTyr1284 Log in to see Polyclonal 0
12.971891 ABIN1877119 IHC WB Rabbit IgG Log in to see Polyclonal 0
12.971891 ABIN5966447 IHC WB Rabbit IgG Log in to see Polyclonal 0
12.971891 ABIN2971563 IF/ICC IHC WB Rabbit C-Term, pThr1284 Log in to see Polyclonal 0
12.971891 ABIN2971565 IF/ICC IHC WB Rabbit C-Term Log in to see Polyclonal 0
12.971891 ABIN2421385 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
12.576219 ABIN3184535 ELISA IF IHC Rabbit IgG Ser36 Log in to see Polyclonal 0
12.576219 ABIN3182801 ELISA IF IHC WB Rabbit IgG pTyr1284 Log in to see Polyclonal 0
12.576219 ABIN2141071 IF IHC ELISA WB Rabbit IgG pTyr1284 Log in to see Polyclonal 0
10 ABIN3048443 IHC WB Rabbit IgG Log in to see Polyclonal 0
9.971891 ABIN1532628 IF IHC ELISA Rabbit IgG AA 1250-1299 Log in to see Polyclonal 0
9.971891 ABIN1048578 IHC IHC (p) Rabbit Internal Region Log in to see Polyclonal 0
9.971891 ABIN2427957 IHC ELISA Rabbit IgG Log in to see Polyclonal 0
7 ABIN2888849 ELISA IF IHC IHC (p) Rabbit IgG AA 1250-1299 Log in to see Polyclonal 0
7 ABIN1576977 IF IHC ELISA Rabbit IgG Log in to see Polyclonal 0
4 ABIN1845331 IF/ICC IHC WB Rabbit pTyr1284 Log in to see Polyclonal 0

General

Antigen V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) Antibodies
Epitope pTyr1284
(42), (35), (34), (33), (20), (18), (17), (17), (15), (13), (12), (12), (7), (6), (6), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(365), (181), (149), (7), (5), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1)
Host Rabbit
(310), (63), (1)
Clonality
Polyclonal
Conjugate This ERBB4 antibody is un-conjugated
(19), (19), (17), (14), (13), (10), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(214), (156), (125), (81), (68), (38), (32), (20), (18), (9), (4), (3), (2), (1), (1)
Supplier Log in to see

Product Details anti-ERBB4 Antibody

Target Details ERBB4 Application Details Handling Images
Specificity Phospho-HER4 (Tyr1284) Antibody detects endogenous levels of HER4 only when phosphorylated at Tyrosine 1284
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen A synthesized peptide derived from human HER4 around the phosphorylation site of Tyrosine 1284
Isotype IgG
Plasmids, Primers & others

Target Details ERBB4

Product Details anti-ERBB4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HER4 (ERBB4 Antibody Abstract)
Background

Description: Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins and EGF family members and regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. Required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. Required for normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. Required for mammary gland differentiation, induction of milk proteins and lactation. Acts as cell-surface receptor for the neuregulins NRG1, NRG2, NRG3 and NRG4 and the EGF family members BTC, EREG and HBEGF. Ligand binding triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Ligand specificity and signaling is modulated by alternative splicing, proteolytic processing, and by the formation of heterodimers with other ERBB family members, thereby creating multiple combinations of intracellular phosphotyrosines that trigger ligand- and context-specific cellular responses. Mediates phosphorylation of SHC1 and activation of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Isoform JM-A CYT-1 and isoform JM-B CYT-1 phosphorylate PIK3R1, leading to the activation of phosphatidylinositol 3-kinase and AKT1 and protect cells against apoptosis. Isoform JM-A CYT-1 and isoform JM-B CYT-1 mediate reorganization of the actin cytoskeleton and promote cell migration in response to NRG1. Isoform JM-A CYT-2 and isoform JM-B CYT-2 lack the phosphotyrosine that mediates interaction with PIK3R1, and hence do not phosphorylate PIK3R1, do not protect cells against apoptosis, and do not promote reorganization of the actin cytoskeleton and cell migration. Proteolytic processing of isoform JM-A CYT-1 and isoform JM-A CYT-2 gives rise to the corresponding soluble intracellular domains (4ICD) that translocate to the nucleus, promote nuclear import of STAT5A, activation of STAT5A, mammary epithelium differentiation, cell proliferation and activation of gene expression. The ERBB4 soluble intracellular domains (4ICD) colocalize with STAT5A at the CSN2 promoter to regulate transcription of milk proteins during lactation. The ERBB4 soluble intracellular domains can also translocate to mitochondria and promote apoptosis.

Gene: ERBB4

Molecular Weight 180kDa
Gene ID 2066
UniProt Q15303
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-ERBB4 Antibody Target Details ERBB4 Handling Images back to top
Application Notes WB 1:500-1:2000 IHC 1:50-1:200 IF/ICC 1:100-1:500
Restrictions For Research Use only

Handling

Product Details anti-ERBB4 Antibody Target Details ERBB4 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

Images

Product Details anti-ERBB4 Antibody Target Details ERBB4 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) (pTyr1284) antibody (ABIN6256632) Western blot analysis of Phospho-HER4 (Tyr1284) expression in various lysates
Western Blotting (WB) image for anti-V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) (pTyr1284) antibody (ABIN6256632) Western blot analysis of HER4 phosphorylation expression in EGF treated HuvEc whole c...
Immunohistochemistry (IHC) image for anti-V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) (pTyr1284) antibody (ABIN6256632) ABIN6267654 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehy...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) (pTyr1284) antibody (ABIN6256632) HeLa cells treated with EGF
Immunofluorescence (fixed cells) (IF/ICC) image for anti-V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4) (pTyr1284) antibody (ABIN6256632) ABIN6267654 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilize...