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|Antigen||Fibroblast Growth Factor 1 (Acidic) (FGF1) Antibodies|
|Conjugate||This FGF1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-FGF1 AntibodyTarget Details FGF1 Application Details Handling Images
|Specificity||Human Fibroblast Growth Factor acidic (hFGF-1)|
|Immunogen||Highly pure (>98%) recombinant hFGF-acidic.|
Target Details FGF1Product Details anti-FGF1 Antibody Application Details Handling Images back to top
|Alternative Name||FGF Acidic / FGF1 (FGF1 Antibody Abstract)|
|Background||The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these two growth factors.Synonyms: Acidic fibroblast growth factor, Beta-endothelial cell growth factor, ECGF-beta, FGFA, HBGF-1, HBGF1, Heparin-binding growth factor 1|
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-FGF1 Antibody Target Details FGF1 Handling Images back to top
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhFGF-acidic (30 ng/mL), a concentration of 0.46 - 0.9 μg/mL of this antibody is required. ELISA: To detect hFGF-acidic by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2 - 0.4 ng/well of recombinant hFGF-acidic. Western Blot: To detect hFGF-acidic by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hFGF-acidic is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-FGF1 Antibody Target Details FGF1 Application Details Images back to top
|Reconstitution||Restore in sterile water to a concentration of 0.1-1.0 mg/mL.|
|Buffer||PBS, pH 7.2 without preservatives.|
|Handling Advice||Centrifuge vial prior to opening!|