|Antigen||Fibroblast Growth Factor 6 (FGF6) Antibodies|
|Conjugate||This FGF6 antibody is un-conjugated Alternatives|
Functional Studies (Func), Neutralization (Neut), ELISA, Western Blotting (WB)
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Product Details anti-FGF6 AntibodyTarget Details FGF6 Application Details Handling Images
|Specificity||Recognizes human, recombinant Fibroblast Growth Factor 6 (FGF6). ~50 % cross-reactivity with rhFGF4, less than 5 % cross-reactivity with rhFGF9 and less than 2 % cross-reactivity with FGF-acidic, rhFGF5 and rhFGF7. In direct ELISA, this antibody shows no cross-reactivity with other cytokines tested.|
Human, recombinant Fibroblast Growth Factor 6 (FGF6) from E. coli.
Type of Immunogen: Recombinant protein
Target Details FGF6Product Details anti-FGF6 Antibody Application Details Handling Images back to top
|Alternative Name||FGF6 (FGF6 Antibody Abstract)|
Name/Gene ID: FGF6
Synonyms: FGF6, FGF-6, Fibroblast growth factor 6, HSTF-2, HBGF-6, HST-2, HST2, HSTF2
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-FGF6 Antibody Target Details FGF6 Handling Images back to top
Approved: ELISA (0.5 - 1 μg/mL), Func, Neut, WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in Neutralization, Western Blot and ELISA. Neutralization: ND50 is ~0.025-0.075 μg/mL in the presence of 1 ng/mL of rhFGF6 and 1 μg/mL of heparin, using the FGF6 responsive NR6R-3T3 fibroblasts as target cells. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. Western Blot: 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human FGF6. The detection limit for rhFGF6 is approximately 2 ng/lane under non-reducing and reducing conditions. ELISA: 0.5-1 μg/mL with the appropriate secondary reagents to detect human FGF6. The detection limit for rhFGF6 is ~0.16 ng/well.
|Restrictions||For Research Use only|
HandlingProduct Details anti-FGF6 Antibody Target Details FGF6 Application Details Images back to top
|Buffer||PBS, pH 7.2, 40 % glycerol.|
|Handling Advice||Avoid repeat freeze-thaw cycles.|
|Storage||4 °C,-20 °C|
Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.