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|Antigen||Fibroblast Growth Factor 7 (FGF7) Antibodies|
|Conjugate||This FGF7 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|1 reference available|
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Product Details anti-FGF7 AntibodyTarget Details FGF7 Application Details Handling References for anti-FGF7 antibody (ABIN116127) Images
|Specificity||This antibody reacts with Human Keratinocyte Growth Factor (KGF).|
|Immunogen||Highly purified (>98%) recombinant FGF-7 (KGF)|
Target Details FGF7Product Details anti-FGF7 Antibody Application Details Handling References for anti-FGF7 antibody (ABIN116127) Images back to top
|Alternative Name||FGF7 / KGF (FGF7 Antibody Abstract)|
|Background||KGF is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.Synonyms: Fibroblast growth factor 7, HBGF7, Heparin-binding growth factor 7, Keratinocyte growth factor|
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-FGF7 Antibody Target Details FGF7 Handling References for anti-FGF7 antibody (ABIN116127) Images back to top
Neutralisation: To yield one-half maximal inhibition [ND50]of the biological activity of hKGF(25 ng/mL), a concentration of 0.56-0.85 μg/mL of this antibody has been used for previouslots. ELISA: To detect hKGF by direct ELISA (using 100 l/well antibody solution) a concentrationof at least 0.5g/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/wellof recombinant hKGF. Western blot: To detect hKGF by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hKGF is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-FGF7 Antibody Target Details FGF7 Application Details References for anti-FGF7 antibody (ABIN116127) Images back to top
|Reconstitution||Restore in sterile water to a concentration of 0.1-1.0 mg/mL.|
|Buffer||PBS, pH 7.2 without preservatives|
|Handling Advice||Avoid repeated freezing and thawing. Centrifuge vial prior to opening!|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.|
References for anti-FGF7 antibody (ABIN116127)Product Details anti-FGF7 Antibody Target Details FGF7 Application Details Handling Images back to top
|Product cited in:||
Lim, Poh, Riau, Beuerman, Tan, Mehta: "Biological and ultrastructural properties of acelagraft, a freeze-dried γ-irradiated human amniotic membrane." in: Archives of ophthalmology, Vol. 128, Issue 10, pp. 1303-10, 2010