anti-Human FGFR2 antibody for Western Blotting

Recommended FGFR2 Antibody (supplied by: Log in to see )

Antigen
Fibroblast Growth Factor Receptor 2 (FGFR2) Antibodies
  • FGFR2
  • FGFR2IIIb
  • bek
  • bfr-1
  • cd332
  • cek3
  • cfd1
  • ect1
  • fgfr-2
  • jws
  • k-sam
  • kgfr
  • tk14
  • tk25
  • xfgfr2
  • DKFZp469H1521
  • BBDS
  • BEK
  • BFR-1
  • CD332
  • CEK3
  • CFD1
  • ECT1
  • JWS
  • K-SAM
  • KGFR
  • TK14
  • TK25
  • AU043015
  • AW556123
  • Bek
  • Fgfr-2
  • Fgfr-7
  • Fgfr7
  • KGFRTr
  • svs
  • FGF-R
  • fgfr72IIIb
  • FGFR
  • fc56c05
  • wu:fc56c05
  • fibroblast growth factor receptor 2
  • fibroblast growth factor receptor 2 L homeolog
  • FGFR2
  • LOC100084641
  • fgfr2
  • Fgfr2
  • fgfr2.L
Epitope
AA 7-37, N-Term
23
23
19
16
13
12
7
7
7
6
6
5
5
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Monkey, Mouse (Murine), Rat (Rattus)
307
104
64
15
6
5
5
4
4
4
3
3
3
1
1
1
Host
Rabbit
211
82
17
15
Clonality
Polyclonal
Conjugate
This FGFR2 antibody is un-conjugated
14
14
11
10
10
9
4
4
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
251
141
130
115
81
65
22
15
11
10
8
2
2
1
1
1
Options
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Catalog No. ABIN391965
$ 335.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1357021 ABIN2788319 WB Rabbit C-Term Log in to see Polyclonal 0
3.1357021 ABIN2788320 WB Rabbit Middle Region Log in to see Polyclonal 0
3.1357021 ABIN391966 FACS IF IHC (p) WB Rabbit Ig Fraction AA 794-821, C-Term Log in to see Polyclonal 5
3.1357021 ABIN560876 IHC (p) ELISA WB Mouse IgG2b kappa AA 621-723, partial Log in to see 1G3 7
3.1357021 ABIN2706148 IHC WB Rabbit Center Log in to see Polyclonal 0
3.1357021 ABIN6271462 ELISA IHC WB Rabbit IgG pTyr769 Log in to see Polyclonal 0
3.1357021 ABIN359856 EIA WB Rabbit Ig Fraction N-Term Log in to see Polyclonal 5
3.1357021 ABIN359854 EIA IHC (p) WB Rabbit Ig Fraction N-Term Log in to see Polyclonal 5
3.1357021 ABIN359855 EIA IHC (p) WB Rabbit Ig Fraction C-Term Log in to see Polyclonal 5
3.1357021 ABIN2464821 ELISA WB Goat Internal Region Log in to see Polyclonal 0
3.1357021 ABIN4311538 FACS ICC IF IHC IHC (p) WB Mouse IgG2b Log in to see 5C5 0
3.1357021 ABIN1533272 IHC ELISA WB Rabbit IgG AA 471-520 Log in to see Polyclonal 0
3.1357021 ABIN1106281 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 2
3.1357021 ABIN391968 FACS IF IHC (p) WB Rabbit Ig Fraction Log in to see Polyclonal 3
3.1357021 ABIN359857 EIA WB Rabbit Ig Fraction Log in to see Polyclonal 4
3.1357021 ABIN5969139 IF IHC (p) WB Rabbit Ig Fraction N-Term Log in to see Polyclonal 0
3.1357021 ABIN5969140 IF IHC (p) WB Rabbit Ig Fraction N-Term Log in to see Polyclonal 0
3.1357021 ABIN391967 FACS IF WB Rabbit Ig Fraction AA 7-37, N-Term Log in to see Polyclonal 0
3.1357021 ABIN6272030 ELISA WB Rabbit IgG pSer782 Log in to see Polyclonal 0
3.1357021 ABIN3183513 ELISA IF IHC WB Rabbit IgG Internal Region Log in to see Polyclonal 0

General

Antigen Fibroblast Growth Factor Receptor 2 (FGFR2) Antibodies
Epitope AA 7-37, N-Term
(23), (23), (19), (16), (13), (12), (7), (7), (7), (6), (6), (5), (5), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Monkey, Mouse (Murine), Rat (Rattus)
(307), (104), (64), (15), (6), (5), (5), (4), (4), (4), (3), (3), (3), (1), (1), (1)
Host Rabbit
(211), (82), (17), (15)
Clonality (Clone)
Polyclonal   ( )
Conjugate This FGFR2 antibody is un-conjugated
(14), (14), (11), (10), (10), (9), (4), (4), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(251), (141), (130), (115), (81), (65), (22), (15), (11), (10), (8), (2), (2), (1), (1), (1)
Pubmed 10 references available
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Product Details anti-FGFR2 Antibody

Target Details FGFR2 Application Details Handling References for anti-FGFR2 antibody (ABIN391965) Images
Specificity This FGFR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15~44 amino acids from the N-terminal region of human FGFR2.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This FGFR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-37 AA from the N-terminal region of human FGFR2.
Clone RB01567
Isotype Ig Fraction
Plasmids, Primers & others

Target Details FGFR2

Product Details anti-FGFR2 Antibody Application Details Handling References for anti-FGFR2 antibody (ABIN391965) Images back to top
Antigen
Alternative Name FGFR2 (FGFR2 Antibody Abstract)
Background FGFR2 is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in the gene are associated with many craniosynostotic syndromes and bone malformations. The genomic organization of the gene encompasses 20 exons. Alternative splicing in multiple exons, including those encoding the Ig-like domains, the transmembrane region and the carboxyl terminus, results in varied isoforms which differ in structure and specificity. Isoform 1 has equal affinity for aFGF and bFGF but does not bind KGF.
Synonyms: fibroblast growth factor receptor 2
Molecular Weight 92025 DA
Gene ID 2263
UniProt P21802
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Growth Factor Binding

Application Details

Product Details anti-FGFR2 Antibody Target Details FGFR2 Handling References for anti-FGFR2 antibody (ABIN391965) Images back to top
Application Notes WB = 1:1000, IF = 1:50-100, FACS = 1:10-50, IHC (p) = 1:50-100
Restrictions For Research Use only

Handling

Product Details anti-FGFR2 Antibody Target Details FGFR2 Application Details References for anti-FGFR2 antibody (ABIN391965) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-FGFR2 antibody (ABIN391965)

Product Details anti-FGFR2 Antibody Target Details FGFR2 Application Details Handling Images back to top
Product cited in:

Guan, Gao, Zhou, Wang, Zheng, Guo, Chang, Li, Wang: "miR-223 Regulates Adipogenic and Osteogenic Differentiation of Mesenchymal Stem Cells Through a C/EBPs/miR-223/FGFR2 Regulatory Feedback Loop." in: Stem cells (Dayton, Ohio), Vol. 33, Issue 5, pp. 1589-600, 2015 Method employed by authors: Western Blotting (WB)

Garcia, Shaffer, Alfaro, Smith, Sun, Zhao, Young, VanSaun, Eid: "Reprogramming of mesenchymal stem cells by the synovial sarcoma-associated oncogene SYT-SSX2." in: Oncogene, Vol. 31, Issue 18, pp. 2323-34, 2012 Method employed by authors: Western Blotting (WB)

Matsui-Inohara, Uematsu, Narita, Satoh, Yonezawa, Kuroda, Ito, Yoneda, Kawarai, Sugiya, Watanabe, Senpuku: "E2F-1-deficient NOD/SCID mice developed showing decreased saliva production." in: Experimental biology and medicine (Maywood, N.J.), Vol. 234, Issue 12, pp. 1525-36, 2009 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Page, Ambady, Holmes, Vilner, Kole, Kashpur, Huntress, Vojtic, Whitton, Dominko: "Induction of stem cell gene expression in adult human fibroblasts without transgenes." in: Cloning and stem cells, Vol. 11, Issue 3, pp. 417-26, 2009 Method employed by authors: Immunocytochemistry (ICC)

Mondrinos, Koutzaki, Lelkes, Finck: "A tissue-engineered model of fetal distal lung tissue." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 293, Issue 3, pp. L639-50, 2007 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Background publications

Freeman, Gangula, Welm, Ozen, Foster, Rosen, Ittmann, Greenberg, Spencer et al.: "Conditional activation of fibroblast growth factor receptor (FGFR) 1, but not FGFR2, in prostate cancer cells leads to increased osteopontin induction, extracellular signal-regulated kinase ..." in: Cancer research, Vol. 63, Issue 19, pp. 6237-43, 2003

Goriely, McVean, Röjmyr, Ingemarsson, Wilkie: "Evidence for selective advantage of pathogenic FGFR2 mutations in the male germ line." in: Science (New York, N.Y.), Vol. 301, Issue 5633, pp. 643-6, 2003

Fomenkov, Huang, Topaloglu, Brechman, Osada, Fomenkova, Yuriditsky, Trink, Sidransky, Ratovitski: "P63 alpha mutations lead to aberrant splicing of keratinocyte growth factor receptor in the Hay-Wells syndrome." in: The Journal of biological chemistry, Vol. 278, Issue 26, pp. 23906-14, 2003

Katoh, Katoh: "Recombination cluster around FGFR2-WDR11-HTPAPL locus on human chromosome 10q26." in: International journal of molecular medicine, Vol. 11, Issue 5, pp. 579-83, 2003

Katoh, Katoh: "FGFR2 and WDR11 are neighboring oncogene and tumor suppressor gene on human chromosome 10q26." in: International journal of oncology, Vol. 22, Issue 5, pp. 1155-9, 2003

Images

Product Details anti-FGFR2 Antibody Target Details FGFR2 Application Details Handling References for anti-FGFR2 antibody (ABIN391965) back to top
Supplier Images
Western Blotting (WB) image for anti-Fibroblast Growth Factor Receptor 2 (FGFR2) (AA 7-37), (N-Term) antibody (ABIN391965) Western blot analysis of anti-hFGFR2-R22 Pab (ABIN391965) in 293 cell line lysates tr...
Immunohistochemistry (IHC) image for anti-Fibroblast Growth Factor Receptor 2 (FGFR2) (AA 7-37), (N-Term) antibody (ABIN391965) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
 image for anti-Fibroblast Growth Factor Receptor 2 (FGFR2) (AA 7-37), (N-Term) antibody (ABIN391965) C:FGFR2/isolectinB4 (C) and FGFR1/isolectinB4 (D) staining of apparent mesenchymal ce...
Flow Cytometry (FACS) image for anti-Fibroblast Growth Factor Receptor 2 (FGFR2) (AA 7-37), (N-Term) antibody (ABIN391965) Overlay histogram showing Hela cells stained with ABIN2841761(green line). The cells ...
Immunofluorescence (IF) image for anti-Fibroblast Growth Factor Receptor 2 (FGFR2) (AA 7-37), (N-Term) antibody (ABIN391965) Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabil...
Immunofluorescence (IF) image for anti-Fibroblast Growth Factor Receptor 2 (FGFR2) (AA 7-37), (N-Term) antibody (ABIN391965) Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabil...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Fibroblast Growth Factor Receptor 2 (FGFR2) (AA 7-37), (N-Term) antibody (ABIN391965) Immunohistochemical analysis of paraffin-embedded H. liver section using FGFR2 Antibo...
Western Blotting (WB) image for anti-Fibroblast Growth Factor Receptor 2 (FGFR2) (AA 7-37), (N-Term) antibody (ABIN391965) All lanes : Anti-FGFR2 Antibody (N-term) at 1:1000-1:2000 dilution Lane 1: DU145 whol...