|Antigen||Fibroblast Growth Factor Receptor 4 (FGFR4) Antibodies|
|Epitope||AA 1-369 Alternatives|
|Conjugate||This FGFR4 antibody is un-conjugated Alternatives|
Cell Culture (CC), ELISA
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Product Details anti-FGFR4 AntibodyTarget Details FGFR4 Application Details Handling Images
Human FGFR4 / CD334
No cross-reactivity in ELISA with
Human cell lysate (293 cell line)
|No Cross-Reactivity||Human, Mouse (Murine)|
|Characteristics||This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human FGFR4 / CD334 (rh FGFR4 / CD334, ABIN2003022 NP_002002.3, Met 1-Asp 369,. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.|
|Purification||purified by Protein A affinity chromatography|
|Sterility||0.2 μm filtered|
|Immunogen||Recombinant Human FGFR4 / CD334 protein (ABIN2003022)|
Target Details FGFR4Product Details anti-FGFR4 Antibody Application Details Handling Images back to top
|Alternative Name||FGFR4 (FGFR4 Antibody Abstract)|
|Background||FGFR4, also designated as CD334, is a member of the fibroblast growth factor receptor subfamily which belongs to the tyrosine protein kinase superfamily. Upon ligand binding, FGFRs undergo dimerization and autophosphorylation, intiating a cascade of downstream signal pathways, ultimately influencing a variety of physiological and pathological cellular processes including mitogenesis and differentiation. FGFR members is highly conserved in amino acid sequence and differ f in their ligand affinities and tissue distribution. FGFR4, a type I transmembrane glycoprotein, contains 3 immunoglobulin-like domains in the extracellular domain and a cytoplasmic tyrosine-kinase domain. FGFR4 expression is detected in gastrointestinal epithelial cells, pancreas, as well as gastric and pancreatic cancer cell lines. FGFR4 is reported to be a high affinity receptor for both acidic and basic fibroblast growth factor, and also binds to FGF19. Like FGF R1- 3,several isoforms of FGFR4 are generated by alternative splicing of the mRNAs. Although its specific function is unknown, growing studies have indicated that FGFR4 plays a role in tumorigenesis and malignace of cetain carcinomas such as breast and ovarian tumors.|
|Research Area||Cancer, Growth Factors, Angiogenesis, Receptors|
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding|
Application DetailsProduct Details anti-FGFR4 Antibody Target Details FGFR4 Handling Images back to top
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human FGFR4. The detection limit for Human FGFR4 is approximately 0.16 ng/well.
ELISA(Cap): 0.5-4 μg/mL In a sandwich ELISA, it can be used as capture antibody.
|Restrictions||For Research Use only|
HandlingProduct Details anti-FGFR4 Antibody Target Details FGFR4 Application Details Images back to top
|Buffer||0.2 μm filtered solution in PBS with 5 % trehalose|
|Handling Advice||Avoid repeated freeze-thaw cycles.|
|Storage||4 °C,-20 °C,-80 °C|
This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
|Expiry Date||12 months|