anti-Human FGFR4 antibody for Cell Culture

Recommended FGFR4 Antibody (supplied by: Log in to see )

Antigen
Fibroblast Growth Factor Receptor 4 (FGFR4) Antibodies
  • FGFR4
  • XFGFR-4
  • XFGFR-4a
  • cd334
  • fgfr-4
  • jtk2
  • tkf
  • xfgfr4
  • CD334
  • JTK2
  • TKF
  • Fgfr-4
  • etID309818.21
  • wu:fc13h10
  • zgc:111935
  • fgfr-4c
  • fgfr4
  • fibroblast growth factor receptor 4
  • FGFR4
  • fgfr4
  • Fgfr4
  • fgfr4-b
Epitope
AA 1-369
20
18
16
13
8
8
7
6
6
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
207
56
45
3
3
Host
Mouse
127
68
27
1
Clonality
Monoclonal
Conjugate
This FGFR4 antibody is un-conjugated
8
8
7
4
4
4
4
4
4
4
3
3
3
1
1
1
1
1
1
1
Application
Cell Culture (CC), ELISA
156
96
55
48
35
29
13
9
9
6
4
3
2
1
1
1
Supplier
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Catalog No. ABIN1997067
$ 218.90
Plus shipping costs $45.00

General

Antigen Fibroblast Growth Factor Receptor 4 (FGFR4) Antibodies
Epitope AA 1-369
(20), (18), (16), (13), (8), (8), (7), (6), (6), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(207), (56), (45), (3), (3)
Host Mouse
(127), (68), (27), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FGFR4 antibody is un-conjugated
(8), (8), (7), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1)
Application Cell Culture (CC), ELISA
(156), (96), (55), (48), (35), (29), (13), (9), (9), (6), (4), (3), (2), (1), (1), (1)
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Product Details anti-FGFR4 Antibody

Target Details FGFR4 Application Details Handling Images
Specificity Human FGFR4 / CD334
No cross-reactivity in ELISA with
Human FGFR1
Human FGFR2
Mouse FGFR3
Human cell lysate (293 cell line)
No Cross-Reactivity Human, Mouse (Murine)
Characteristics This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human FGFR4 / CD334 (rh FGFR4 / CD334, ABIN2003022 NP_002002.3, Met 1-Asp 369,. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Purification purified by Protein A affinity chromatography
Sterility 0.2 μm filtered
Immunogen Recombinant Human FGFR4 / CD334 protein (ABIN2003022)
Clone 01
Isotype IgG1

Target Details FGFR4

Product Details anti-FGFR4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name FGFR4 (FGFR4 Antibody Abstract)
Background FGFR4, also designated as CD334, is a member of the fibroblast growth factor receptor subfamily which belongs to the tyrosine protein kinase superfamily. Upon ligand binding, FGFRs undergo dimerization and autophosphorylation, intiating a cascade of downstream signal pathways, ultimately influencing a variety of physiological and pathological cellular processes including mitogenesis and differentiation. FGFR members is highly conserved in amino acid sequence and differ f in their ligand affinities and tissue distribution. FGFR4, a type I transmembrane glycoprotein, contains 3 immunoglobulin-like domains in the extracellular domain and a cytoplasmic tyrosine-kinase domain. FGFR4 expression is detected in gastrointestinal epithelial cells, pancreas, as well as gastric and pancreatic cancer cell lines. FGFR4 is reported to be a high affinity receptor for both acidic and basic fibroblast growth factor, and also binds to FGF19. Like FGF R1- 3,several isoforms of FGFR4 are generated by alternative splicing of the mRNAs. Although its specific function is unknown, growing studies have indicated that FGFR4 plays a role in tumorigenesis and malignace of cetain carcinomas such as breast and ovarian tumors.
NCBI Accession NP_002002.3
Research Area Cancer, Growth Factors, Angiogenesis, Receptors
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding

Application Details

Product Details anti-FGFR4 Antibody Target Details FGFR4 Handling Images back to top
Application Notes ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human FGFR4. The detection limit for Human FGFR4 is approximately 0.16 ng/well.
ELISA(Cap): 0.5-4 μg/mL In a sandwich ELISA, it can be used as capture antibody.
Restrictions For Research Use only

Handling

Product Details anti-FGFR4 Antibody Target Details FGFR4 Application Details Images back to top
Format Liquid
Buffer 0.2 μm filtered solution in PBS with 5 % trehalose
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C,-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Expiry Date 12 months