anti-Human FLT1 antibody for Cytometry by Time of Flight

Recommended FLT1 Antibody (supplied by: Log in to see )

Antigen
Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) Antibodies
  • FLT
  • FLT-1
  • VEGFR-1
  • VEGFR1
  • FLT1
  • flt1b
  • sFlt1
  • AI323757
  • Flt-1
  • fms related tyrosine kinase 1
  • FMS-related tyrosine kinase 1
  • vascular endothelial growth factor receptor 1
  • fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)
  • FMS-like tyrosine kinase 1
  • FLT1
  • Flt1
  • CpipJ_CPIJ012087
  • flt1
Epitope
AA 27-687
43
34
17
16
14
12
12
8
8
7
6
5
5
5
5
5
5
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
435
149
114
17
17
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Host
Goat
253
250
13
5
2
1
Clonality
Polyclonal
Conjugate
This FLT1 antibody is un-conjugated
35
20
10
10
10
9
9
7
7
7
6
6
6
6
6
5
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
306
196
148
102
101
77
60
40
22
13
13
10
9
3
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN5013028
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4900044 CyTOF FACS WB Mouse IgG1 AA 27-687 Log in to see 49560 8

General

Antigen Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) Antibodies
Epitope AA 27-687
(43), (34), (17), (16), (14), (12), (12), (8), (8), (7), (6), (5), (5), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(435), (149), (114), (17), (17), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Host Goat
(253), (250), (13), (5), (2), (1)
Clonality
Polyclonal
Conjugate This FLT1 antibody is un-conjugated
(35), (20), (10), (10), (10), (9), (9), (7), (7), (7), (6), (6), (6), (6), (6), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
(306), (196), (148), (102), (101), (77), (60), (40), (22), (13), (13), (10), (9), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 23 references available
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Product Details anti-FLT1 Antibody

Target Details FLT1 Application Details Handling References for anti-FLT1 antibody (ABIN5013028) Images
Specificity Detects human VEGF R1/Flt-1 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 20 % cross-reactivity with recombinant mouse VEGF R1 and less than 10 % cross-reactivity with recombinant human (rh) VEGF R2 and rhVEGF R3.
Purification Immunogen affinity purified
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGF R1/Flt-1, Ser27-His687, Accession # NP_001153392
Isotype IgG
Plasmids, Primers & others

Target Details FLT1

Product Details anti-FLT1 Antibody Application Details Handling References for anti-FLT1 antibody (ABIN5013028) Images back to top
Antigen
Alternative Name VEGF R1/Flt-1 (FLT1 Antibody Abstract)
Background Alternative Names: EC 2.7.10, EC 2.7.10.1, FLT, FLT1, FLT-1, Fms-like tyrosine kinase 1, fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor), FRT, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, vascular endothelial growth factor receptor 1, Vascular permeability factor receptor, VEGFR1, VEGFR-1
Gene ID 2321
NCBI Accession NP_001153392
Pathways RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals

Application Details

Product Details anti-FLT1 Antibody Target Details FLT1 Handling References for anti-FLT1 antibody (ABIN5013028) Images back to top
Application Notes Western Blot 0.1 μg/mL
Flow Cytometry 2.5 μg/10^6 cells
Immunohistochemistry 5-15 μg/mL
Blockade of Receptor-ligand Interaction
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-FLT1 Antibody Target Details FLT1 Application Details References for anti-FLT1 antibody (ABIN5013028) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-FLT1 antibody (ABIN5013028)

Product Details anti-FLT1 Antibody Target Details FLT1 Application Details Handling Images back to top
Product cited in:

Basavarajappa, Sulaiman, Qi, Shetty, Sheik Pran Babu, Sishtla, Lee, Quigley, Alkhairy, Briggs, Gupta, Tang, Shadmand, Grant, Boulton, Seo, Corson: "Ferrochelatase is a therapeutic target for ocular neovascularization." in: EMBO molecular medicine, Vol. 9, Issue 6, pp. 786-801, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Szalai, Romero, Chaiworapongsa, Xu, Wang, Ahn, Xu, Chiang, Sundell, Wang, Jiang, Plazyo, Olive, Tarca, Dong, Qureshi, Papp, Hassan, Hernandez-Andrade, Than: "Full-length human placental sFlt-1-e15a isoform induces distinct maternal phenotypes of preeclampsia in mice." in: PLoS ONE, Vol. 10, Issue 4, pp. e0119547, 2016 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Kim, Kim, Kim, Cho, Lee: "The effect of vascular endothelial growth factor on osteoclastogenesis in rheumatoid arthritis." in: PLoS ONE, Vol. 10, Issue 4, pp. e0124909, 2016 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Chanakira, Kir, Barke, Santilli, Ramakrishnan, Roy: "Hypoxia Differentially Regulates Arterial and Venous Smooth Muscle Cell Migration." in: PLoS ONE, Vol. 10, Issue 9, pp. e0138587, 2016 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Shvartsman, Storrie-White, Lee, Kearney, Brudno, Ho, Cezar, McCann, Anderson, Koullias, Tapia, Vandenburgh, Lichtman, Mooney: "Sustained delivery of VEGF maintains innervation and promotes reperfusion in ischemic skeletal muscles via NGF/GDNF signaling." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 22, Issue 7, pp. 1243-1253, 2015 Method employed by authors: Functional Studies (Func) (Sample species: Human).

Hou, Cheng, Chung, Kuo, Yeh, Chang, Lu, Wang, Yu: "Elastase induces lung epithelial cell autophagy through placental growth factor: a new insight of emphysema pathogenesis." in: Autophagy, Vol. 10, Issue 9, pp. 1509-21, 2015 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Nevo, Lee, Caniggia: "Attenuation of VEGFR-2 expression by sFlt-1 and low oxygen in human placenta." in: PLoS ONE, Vol. 8, Issue 11, pp. e81176, 2014 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Larson, Lee, Lezcano, Zhan, Huang, Fischer, Murphy: "Melanoma spheroid formation involves laminin-associated vasculogenic mimicry." in: The American journal of pathology, Vol. 184, Issue 1, pp. 71-8, 2014 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Human).

Lezcano, Kleffel, Lee, Larson, Zhan, DoRosario, Wang, Schatton, Murphy: "Merkel cell carcinoma expresses vasculogenic mimicry: demonstration in patients and experimental manipulation in xenografts." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 94, Issue 10, pp. 1092-102, 2014 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Human).

Vizio, Biasi, Scirelli, Novarino, Prati, Ciuffreda, Montrucchio, Poli, Bellone: "Pancreatic-carcinoma-cell-derived pro-angiogenic factors can induce endothelial-cell differentiation of a subset of circulating CD34+ progenitors." in: Journal of translational medicine, Vol. 11, pp. 314, 2013 Method employed by authors: Blocking Peptide (BP) (Sample species: Human).

Cai, Chen, Ruan, Han, Liu, Qi, Boye, Hauswirth, Grant, Boulton: "γ-Secretase and presenilin mediate cleavage and phosphorylation of vascular endothelial growth factor receptor-1." in: The Journal of biological chemistry, Vol. 286, Issue 49, pp. 42514-23, 2012 Method employed by authors: Proximity Ligation Assay (PLA) (Sample species: Cow (Bovine)).

Munaut, Lorquet, Pequeux, Coulon, Le Goarant, Chantraine, Noël, Goffin, Tsatsaris, Subtil, Foidart: "Differential expression of Vegfr-2 and its soluble form in preeclampsia." in: PLoS ONE, Vol. 7, Issue 3, pp. e33475, 2012 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Barsoum, Renaud, Graham: "Glyceryl trinitrate inhibits hypoxia-induced release of soluble fms-like tyrosine kinase-1 and endoglin from placental tissues." in: The American journal of pathology, Vol. 178, Issue 6, pp. 2888-96, 2011 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Byeon, Lee, Choi, Lee, Lee, Chu, Kwon: "Vascular endothelial growth factor as an autocrine survival factor for retinal pigment epithelial cells under oxidative stress via the VEGF-R2/PI3K/Akt." in: Investigative ophthalmology & visual science, Vol. 51, Issue 2, pp. 1190-7, 2010 Method employed by authors: Immunocytochemistry (ICC) (Sample species: Human).

Lee, Myers, Kim: "Vascular endothelial growth factor drives autocrine epithelial cell proliferation and survival in chronic rhinosinusitis with nasal polyposis." in: American journal of respiratory and critical care medicine, Vol. 180, Issue 11, pp. 1056-67, 2009 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Akintoye, Lam, Shi, Brahim, Collins, Robey: "Skeletal site-specific characterization of orofacial and iliac crest human bone marrow stromal cells in same individuals." in: Bone, Vol. 38, Issue 6, pp. 758-68, 2007 Method employed by authors: Immunocytochemistry (ICC) (Sample species: Human).

Ball, Shuttleworth, Kielty: "Vascular endothelial growth factor can signal through platelet-derived growth factor receptors." in: The Journal of cell biology, Vol. 177, Issue 3, pp. 489-500, 2007 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Getmanova, Chen, Bloom, Gokemeijer, Shamah, Warikoo, Wang, Ling, Sun: "Antagonists to human and mouse vascular endothelial growth factor receptor 2 generated by directed protein evolution in vitro." in: Chemistry & biology, Vol. 13, Issue 5, pp. 549-56, 2006 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Cianfarani, Zambruno, Brogelli, Sera, Lacal, Pesce, Capogrossi, Failla, Napolitano, Odorisio: "Placenta growth factor in diabetic wound healing: altered expression and therapeutic potential." in: The American journal of pathology, Vol. 169, Issue 4, pp. 1167-82, 2006 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Asikainen, Schneider, Waleh, Clyman, Ho, Flippin, Günzler, White: "Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 29, pp. 10212-7, 2005 Method employed by authors: Immunocytochemistry (ICC) (Sample species: Human).

Images

Product Details anti-FLT1 Antibody Target Details FLT1 Application Details Handling References for anti-FLT1 antibody (ABIN5013028) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) (AA 27-687) antibody (ABIN5013028) VEGF R1/Flt-1 in Human Breast Cancer Tissue. VEGF R1/Flt-1 was detected in immersion ...
Immunohistochemistry (IHC) image for anti-Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) (AA 27-687) antibody (ABIN5013028) VEGF R1/Flt-1 in Human Ovarian Cancer Tissue. VEGF R1/Flt-1 was detected in immersion...