anti-Human FLT1 antibody for Western Blotting

Recommended FLT1 Antibody (supplied by: Log in to see )

Antigen
Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) Antibodies
  • FLT
  • FLT-1
  • VEGFR-1
  • VEGFR1
  • FLT1
  • flt1b
  • sFlt1
  • AI323757
  • Flt-1
  • fms related tyrosine kinase 1
  • FMS-related tyrosine kinase 1
  • vascular endothelial growth factor receptor 1
  • fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)
  • FMS-like tyrosine kinase 1
  • FLT1
  • Flt1
  • CpipJ_CPIJ012087
  • flt1
Epitope
Internal Region, AA 800-900
49
39
16
15
14
14
13
11
10
9
6
5
5
5
5
5
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
416
151
90
19
13
4
3
2
2
2
2
2
2
2
1
1
1
1
1
Host
Rabbit
241
214
36
5
4
1
Clonality
Polyclonal
Conjugate
This FLT1 antibody is un-conjugated
29
14
14
13
13
12
10
9
9
8
8
8
8
8
7
5
5
5
5
4
3
2
2
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
321
228
106
74
69
59
56
41
14
13
13
6
5
4
4
3
3
2
2
1
1
1
1
1
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Catalog No. ABIN151960
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.232922 ABIN725795 IF (p) IHC (p) WB Rabbit IgG AA 1160-1210 Log in to see Polyclonal 3
3.232922 ABIN2705326 WB Rabbit C-Term, pTyr1213 Log in to see Polyclonal 0
3.232922 ABIN1531410 IHC ELISA WB Rabbit IgG AA 1289-1338, pTyr1333 Log in to see Polyclonal 1
3.232922 ABIN449642 IP ELISA WB Mouse IgG1 Log in to see Flt-1-EWI 0
3.232922 ABIN2788655 WB Rabbit C-Term Log in to see Polyclonal 0
3.232922 ABIN955511 EIA FACS IHC (p) WB Rabbit Ig Fraction Log in to see Polyclonal 1
3.232922 ABIN3042716 WB Rabbit IgG AA 1299-1317, C-Term Log in to see Polyclonal 0
3.232922 ABIN269284 ELISA ICC IF WB Mouse IgG1 Log in to see Flt-1-EWC 1
3.232922 ABIN269286 ICC IF IP ELISA WB Mouse IgG1 Log in to see EWF 0
3.232922 ABIN969146 ELISA WB Mouse IgG1 Log in to see 3D10 2
3.232922 ABIN3183067 ELISA WB Rabbit IgG pTyr1213 Log in to see Polyclonal 0
3.232922 ABIN3182563 ELISA IHC WB Rabbit IgG pTyr1333 Log in to see Polyclonal 0
3.232922 ABIN1175060 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 27-328 Log in to see Polyclonal 0
3.232922 ABIN449639 ICC IF IP ELISA WB Rabbit Log in to see Polyclonal 0
3.232922 ABIN1876809 WB Rabbit IgG Log in to see Polyclonal 0
3.232922 ABIN515759 PLA WB Rabbit AA 1-687, full length Log in to see Polyclonal 0
3.232922 ABIN4251393 WB Mouse IgG1 Log in to see 8G6 0
3.232922 ABIN515758 IF WB Mouse AA 1-687, full length Log in to see Polyclonal 0
3.232922 ABIN2801854 WB Rabbit IgG Log in to see Polyclonal 2
3.232922 ABIN4251373 ELISA WB Mouse IgG1 Log in to see 3D10 0

General

Antigen Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) Antibodies
Epitope Internal Region, AA 800-900
(49), (39), (16), (15), (14), (14), (13), (11), (10), (9), (6), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(416), (151), (90), (19), (13), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(241), (214), (36), (5), (4), (1)
Clonality
Polyclonal
Conjugate This FLT1 antibody is un-conjugated
(29), (14), (14), (13), (13), (12), (10), (9), (9), (8), (8), (8), (8), (8), (7), (5), (5), (5), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(321), (228), (106), (74), (69), (59), (56), (41), (14), (13), (13), (6), (5), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-FLT1 Antibody

Target Details FLT1 Application Details Handling References for anti-FLT1 antibody (ABIN151960) Images
Specificity This antibody targets VEGFR-1 but has significant cross-reactivity with the VEGFR-2 protein.
Purification Immunogen affinity purified
Immunogen A synthetic peptide made to an internal region of the mouse VEGF Receptor 1 protein (between residues 800-900) [UniProt# P35969]. There is 87% identity between the immunogen used for this production and the VEGF Receptor 2 protein.

Target Details FLT1

Product Details anti-FLT1 Antibody Application Details Handling References for anti-FLT1 antibody (ABIN151960) Images back to top
Antigen
Alternative Name VEGFR1/Flt-1 (FLT1 Antibody Abstract)
Background Gene Symbol: FLT1
Gene ID 2321
UniProt P35969
Pathways RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals

Application Details

Product Details anti-FLT1 Antibody Target Details FLT1 Handling References for anti-FLT1 antibody (ABIN151960) Images back to top
Application Notes Western Blot 1:100-1:2000, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:500, Immunohistochemistry-ParaffinThis VEGF Receptor 1 antibody is useful for Western blot, where a band is seen at ~180-230 kDa (the theoretical molecular weight of mouse VEGFR1 is ~150 kDa and human VEGFR1 is ~151 kDa). The difference in MWs is likely due to glycosylation or other post translational modifications. With CSF-1R/VEGFR2 chimera transfected lysates a doublet is seen at ~150 kDa representing VEGFR-2. An unknown band is also seen at ~50 kDa. Optimal working dilutions should be determined by the investigator.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for VEGF Receptor 1 Antibody Western Blot Protocol
1. Perform SDS-PAGE (3-8 %) on samples to be analyzed, loading 50 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 0.5 % BSA in TBS for 1 hour.
. Dilute the rabbit anti-VEGFR1 primary antibody in blocking buffer and incubate 2 hours at room temperature.
. Wash the membrane in water for 5 minutes and apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBS containing 0.05-0.1 % Tween-20 for 10-20 minutes.
. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Amersham's ECL is the standard reagent used).Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only

Handling

Product Details anti-FLT1 Antibody Target Details FLT1 Application Details References for anti-FLT1 antibody (ABIN151960) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

References for anti-FLT1 antibody (ABIN151960)

Product Details anti-FLT1 Antibody Target Details FLT1 Application Details Handling Images back to top
Product cited in:

Puglisi, Puppin, Pegolo, Andreetta, Pascoletti, DAurizio, Pandolfi, Fasola, Piga, Damante, Di Loreto: "Expression of periostin in human breast cancer." in: Journal of clinical pathology, Vol. 61, Issue 4, pp. 494-8, 2008

Ptaszynska, Pendrak, Bandle, Stracke, Roberts: "Positive feedback between vascular endothelial growth factor-A and autotaxin in ovarian cancer cells." in: Molecular cancer research : MCR, Vol. 6, Issue 3, pp. 352-63, 2008 (Sample species: Human). Further details: Western Blotting

Takahashi, Hattori, Iwamatsu, Takizawa, Shibuya: "A novel snake venom vascular endothelial growth factor (VEGF) predominantly induces vascular permeability through preferential signaling via VEGF receptor-1." in: The Journal of biological chemistry, Vol. 279, Issue 44, pp. 46304-14, 2004

Rahimi, Dayanir, Lashkari: "Receptor chimeras indicate that the vascular endothelial growth factor receptor-1 (VEGFR-1) modulates mitogenic activity of VEGFR-2 in endothelial cells." in: The Journal of biological chemistry, Vol. 275, Issue 22, pp. 16986-92, 2000

Background publications

Gluzman-Poltorak, Cohen, Shibuya, Neufeld: "Vascular endothelial growth factor receptor-1 and neuropilin-2 form complexes." in: The Journal of biological chemistry, Vol. 276, Issue 22, pp. 18688-94, 2001

Images

Product Details anti-FLT1 Antibody Target Details FLT1 Application Details Handling References for anti-FLT1 antibody (ABIN151960) back to top
Supplier Images
Western Blotting (WB) image for anti-Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) (AA 800-900), (Internal Region) antibody (ABIN151960) Chimeric CSF-1R/VEGFR-2 detection in transfected lysates.
Immunofluorescence (IF) image for anti-Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) (AA 800-900), (Internal Region) antibody (ABIN151960) Immunocytochemistry/Immunofluorescence: VEGF R1/Flt-1 Antibody [ABIN151960] - VEGF R1...
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) image for anti-Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) (AA 800-900), (Internal Region) antibody (ABIN151960) Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody - IHC analysis of a formalin-fi...
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) image for anti-Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) (AA 800-900), (Internal Region) antibody (ABIN151960) Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody - IHC analysis of a formalin-fi...