anti-Mouse (Murine) FLT1 antibody for ELISA (Capture)

Recommended FLT1 Antibody (supplied by: Log in to see )

Antigen
Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) Antibodies
  • FLT
  • FLT-1
  • VEGFR-1
  • VEGFR1
  • FLT1
  • flt1b
  • sFlt1
  • AI323757
  • Flt-1
  • fms related tyrosine kinase 1
  • FMS-related tyrosine kinase 1
  • vascular endothelial growth factor receptor 1
  • fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor)
  • FMS-like tyrosine kinase 1
  • FLT1
  • Flt1
  • CpipJ_CPIJ012087
  • flt1
Epitope
AA 27-759
48
37
16
16
14
13
12
11
10
9
6
5
5
5
5
5
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
416
149
89
19
13
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Host
Goat
242
214
35
5
2
1
Clonality
Polyclonal
Conjugate
This FLT1 antibody is un-conjugated
29
14
14
13
13
12
10
9
9
8
8
8
8
8
7
5
5
5
5
4
3
2
2
1
1
1
1
1
1
1
1
Application
Blocking Reagent (BR), ELISA (Capture), Flow Cytometry (FACS), Neutralization (Neut), Western Blotting (WB)
319
227
107
73
68
59
59
42
13
13
12
8
5
4
3
3
3
2
1
1
1
1
1
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Catalog No. ABIN5013029
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General

Antigen Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) Antibodies
Epitope AA 27-759
(48), (37), (16), (16), (14), (13), (12), (11), (10), (9), (6), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(416), (149), (89), (19), (13), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Host Goat
(242), (214), (35), (5), (2), (1)
Clonality
Polyclonal
Conjugate This FLT1 antibody is un-conjugated
(29), (14), (14), (13), (13), (12), (10), (9), (9), (8), (8), (8), (8), (8), (7), (5), (5), (5), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Blocking Reagent (BR), ELISA (Capture), Flow Cytometry (FACS), Neutralization (Neut), Western Blotting (WB)
(319), (227), (107), (73), (68), (59), (59), (42), (13), (13), (12), (8), (5), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Pubmed 25 references available
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Product Details anti-FLT1 Antibody

Target Details FLT1 Application Details Handling References for anti-FLT1 antibody (ABIN5013029) Images
Specificity Detects mouse VEGF R1/Flt-1 in ELISAs and Western blots. In sandwich ELISAs, approximately 7 % cross-reactivity with recombinant human VEGF R1 is observed, approximately 4 % cross-reactivity with recombinant mouse (rm) VEGF164 is observed, less than 1 % cross-reactivity with recombinant rat VEGF164 is observed, and less than 0.2 % cross-reactivity with rmVEGF R2 and rmVEGF R3 is observed.
Purification Immunogen affinity purified
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen Mouse myeloma cell line NS0-derived recombinant mouse VEGF R1/Flt-1, Ser27-Glu759, Accession # P35969
Isotype IgG

Target Details FLT1

Product Details anti-FLT1 Antibody Application Details Handling References for anti-FLT1 antibody (ABIN5013029) Images back to top
Antigen
Alternative Name VEGF R1/Flt-1 (FLT1 Antibody Abstract)
Background Alternative Names: EC 2.7.10, EC 2.7.10.1, FLT, FLT1, FLT-1, Fms-like tyrosine kinase 1, fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor), FRT, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, vascular endothelial growth factor receptor 1, Vascular permeability factor receptor, VEGFR1, VEGFR-1
Gene ID 2321
UniProt P35969
Pathways RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals

Application Details

Product Details anti-FLT1 Antibody Target Details FLT1 Handling References for anti-FLT1 antibody (ABIN5013029) Images back to top
Application Notes Western Blot 0.1 μg/mL
Flow Cytometry 2.5 μg/10^6 cells
Blockade of Receptor-ligand Interaction
Neutralization 2-8 μg/mL
CyTOF-ready
ELISA Capture (Matched Antibody Pair) 0.2-0.8 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-FLT1 Antibody Target Details FLT1 Application Details References for anti-FLT1 antibody (ABIN5013029) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-FLT1 antibody (ABIN5013029)

Product Details anti-FLT1 Antibody Target Details FLT1 Application Details Handling Images back to top
Product cited in:

Teixeira, Lopes, Gimenes, Teixeira, da Silva, Brígido, da Luz, da Silva, Silva, Florentino, Tavares, Dos Santos, Ávila, Silva, Elias, Mortara, da Silva: "Mechanistic Insights into the Anti-angiogenic Activity of Trypanosoma cruzi Protein 21 and its Potential Impact on the Onset of Chagasic Cardiomyopathy." in: Scientific reports, Vol. 7, pp. 44978, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Ola, Dubrac, Han, Zhang, Fang, Larrivée, Lee, Urarte, Kraehling, Genet, Hirschi, Sessa, Canals, Graupera, Yan, Young, Oh, Eichmann: "PI3 kinase inhibition improves vascular malformations in mouse models of hereditary haemorrhagic telangiectasia." in: Nature communications, Vol. 7, pp. 13650, 2016 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).

Hou, Nilchi, Li, Gangaraju, Jiang, Aylsworth, Monette, Slinn: "Semaphorin3A elevates vascular permeability and contributes to cerebral ischemia-induced brain damage." in: Scientific reports, Vol. 5, pp. 7890, 2015 Method employed by authors: Biochemical Assay (BCA) (Sample species: Rat (Rattus)).

Hudson, Powner, Sarker, Burgoyne, Campbell, Ockrim, Martinelli, Futter, Grant, Fraser, Shima, Greenwood, Turowski: "Differential apicobasal VEGF signaling at vascular blood-neural barriers." in: Developmental cell, Vol. 30, Issue 5, pp. 541-52, 2014 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Mouse (Murine)).

Yao, Duan, Yang, Buch: "Nonmuscle myosin light-chain kinase mediates microglial migration induced by HIV Tat: involvement of β1 integrins." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 27, Issue 4, pp. 1532-48, 2013 Method employed by authors: Neutralization (Neut) (Sample species: Rat (Rattus)).

Carmi, Dotan, Rider, Kaplanov, White, Baron, Abutbul, Huszar, Dinarello, Apte, Voronov: "The role of IL-1β in the early tumor cell-induced angiogenic response." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 190, Issue 7, pp. 3500-9, 2013

Schreurs, Houston, May, Cipolla: "The adaptation of the blood-brain barrier to vascular endothelial growth factor and placental growth factor during pregnancy." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 26, Issue 1, pp. 355-62, 2012 Method employed by authors: In vivo Studies (in vivo) (Sample species: Rat (Rattus)).

Benedito, Rocha, Woeste, Zamykal, Radtke, Casanovas, Duarte, Pytowski, Adams: "Notch-dependent VEGFR3 upregulation allows angiogenesis without VEGF-VEGFR2 signalling." in: Nature, Vol. 484, Issue 7392, pp. 110-4, 2012 Method employed by authors: Immunoprecipitation (IP) (Sample species: Mouse (Murine)).

Roda, Sumner, Evans, Phillips, Marsh, Eubank: "Hypoxia-inducible factor-2α regulates GM-CSF-derived soluble vascular endothelial growth factor receptor 1 production from macrophages and inhibits tumor growth and angiogenesis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 4, pp. 1970-6, 2011 Method employed by authors: In vivo Studies (in vivo) (Sample species: Mouse (Murine)).

Eubank, Roberts, Khan, Curry, Nuovo, Kuppusamy, Marsh: "Granulocyte macrophage colony-stimulating factor inhibits breast cancer growth and metastasis by invoking an anti-angiogenic program in tumor-educated macrophages." in: Cancer research, Vol. 69, Issue 5, pp. 2133-40, 2009 Method employed by authors: In vivo Studies (in vivo) (Sample species: Mouse (Murine)).

Shin, Yoon, Kim, Kim, Park: "Vascular endothelial growth factor-induced chemotaxis and IL-10 from T cells." in: Cellular immunology, Vol. 256, Issue 1-2, pp. 72-8, 2009 Method employed by authors: Neutralization (Neut) (Sample species: Mouse (Murine)).

Zhang, Ramirez, Yankeelov, Li, Ford, Qi, Pozzi, Zutter: "alpha2beta1 integrin expression in the tumor microenvironment enhances tumor angiogenesis in a tumor cell-specific manner." in: Blood, Vol. 111, Issue 4, pp. 1980-8, 2008 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Li, Zhang, Nagai, Tang, Zhang, Scotney, Lennartsson, Zhu, Qu, Fang, Hua, Matsuo, Fong, Ding, Cao, Becker, Nash, Heldin, Li: "VEGF-B inhibits apoptosis via VEGFR-1-mediated suppression of the expression of BH3-only protein genes in mice and rats." in: The Journal of clinical investigation, Vol. 118, Issue 3, pp. 913-23, 2008 Method employed by authors: In vivo Studies (in vivo) (Sample species: Mouse (Murine)).

Marko, Damon: "VEGF promotes vascular sympathetic innervation." in: American journal of physiology. Heart and circulatory physiology, Vol. 294, Issue 6, pp. H2646-52, 2008 Method employed by authors: Immunocytochemistry (ICC) (Sample species: Rat (Rattus)).

Nozaki, Sakurai, Raisler, Baffi, Witta, Ogura, Brekken, Sage, Ambati, Ambati: "Loss of SPARC-mediated VEGFR-1 suppression after injury reveals a novel antiangiogenic activity of VEGF-A." in: The Journal of clinical investigation, Vol. 116, Issue 2, pp. 422-9, 2006 Method employed by authors: Immunoprecipitation (IP) (Sample species: Mouse (Murine)).

Le Bras, Barallobre, Homman-Ludiye, Ny, Wyns, Tammela, Haiko, Karkkainen, Yuan, Muriel, Chatzopoulou, Bréant, Zalc, Carmeliet, Alitalo, Eichmann, Thomas: "VEGF-C is a trophic factor for neural progenitors in the vertebrate embryonic brain." in: Nature neuroscience, Vol. 9, Issue 3, pp. 340-8, 2006 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Mouse (Murine)).

Cianfarani, Zambruno, Brogelli, Sera, Lacal, Pesce, Capogrossi, Failla, Napolitano, Odorisio: "Placenta growth factor in diabetic wound healing: altered expression and therapeutic potential." in: The American journal of pathology, Vol. 169, Issue 4, pp. 1167-82, 2006 Method employed by authors: ELISA (ELISA) (Sample species: Mouse (Murine)).

Ambati, Nozaki, Singh, Takeda, Jani, Suthar, Albuquerque, Richter, Sakurai, Newcomb, Kleinman, Caldwell, Lin, Ogura, Orecchia, Samuelson, Agnew, St Leger, Green, Mahasreshti, Curiel, Kwan, Marsh et al.: "Corneal avascularity is due to soluble VEGF receptor-1. ..." in: Nature, Vol. 443, Issue 7114, pp. 993-7, 2006 Method employed by authors: In vivo Studies (in vivo) (Sample species: Mouse (Murine)).

Mohamedali, Poblenz, Sikes, Navone, Thorpe, Darnay, Rosenblum: "Inhibition of prostate tumor growth and bone remodeling by the vascular targeting agent VEGF121/rGel." in: Cancer research, Vol. 66, Issue 22, pp. 10919-28, 2006 Method employed by authors: Neutralization (Neut) (Sample species: Mouse (Murine)).

Gudehithlu, Ahmed, Wu, Litbarg, Garber, Arruda, Dunea, Singh: "Antagonism of vascular endothelial growth factor results in microvessel attrition and disorganization of wound tissue." in: The Journal of laboratory and clinical medicine, Vol. 145, Issue 4, pp. 194-203, 2005 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Rat (Rattus)).

Images

Product Details anti-FLT1 Antibody Target Details FLT1 Application Details Handling References for anti-FLT1 antibody (ABIN5013029) back to top
Supplier Images
Neutralization (Neut) image for anti-Fms-Related tyrosine Kinase 1 (Vascular Endothelial Growth Factor/vascular Permeability Factor Receptor) (FLT1) (AA 27-759) antibody (ABIN5013029) Cell Proliferation Induced by VEGF164 and Neutralization by Mouse VEGF R1/Flt-1 Antib...