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|Antigen||Fms-Related tyrosine Kinase 3 (FLT3) Antibodies|
|Epitope||AA 1-200 Alternatives|
|Conjugate||This FLT3 antibody is un-conjugated Alternatives|
Immunoprecipitation (IP), ELISA, Western Blotting (WB)
|1 reference available|
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Product Details anti-FLT3 AntibodyTarget Details FLT3 Application Details Handling References for anti-FLT3 antibody (ABIN1996829) Images
|Specificity||Human FLT3 / FLK2 / CD135|
|Characteristics||Produced in rabbits immunized with purified, recombinant Human FLT3 (rh FLT3, ABIN2002759 NP_116171.3, Met 1-Arg 200,. FLT3 specific IgG was purified by human FLT3 affinity chromatography .|
|Purification||purified by affinity chromatography|
|Sterility||0.2 μm filtered|
|Immunogen||Recombinant Human FLT3 protein (ABIN2002759)|
Target Details FLT3Product Details anti-FLT3 Antibody Application Details Handling References for anti-FLT3 antibody (ABIN1996829) Images back to top
|Alternative Name||FLT3 (FLT3 Antibody Abstract)|
|Background||The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD135, also known as FLT-3, FLK-2, is a member of the CD system. CD135 is an important cell surface marker recognized by specific sets of antibodies to identify the types of hematopoietic (blood) progenitors in the bone marrow and it function to differentiate hematopoietic stem cells, which are CD135 negative, from multipotent progenitors, which are CD135 positive. CD135 is a receptor tyrosine kinase typeIII for the cytokine Flt3 ligand and activat signaling through second messengers by binding to Flt3. Signaling through CD135 is important for lymphocyte development. The encoding gene CD135 is a proto-oncogene to which mutations happened will lead to cancer such as leukemia.|
Application DetailsProduct Details anti-FLT3 Antibody Target Details FLT3 Handling References for anti-FLT3 antibody (ABIN1996829) Images back to top
WB: 5-10 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human FLT3. The detection limit for Human FLT3 is approximately 0.00975 ng/well.
IP: 1-4 μg/mg of lysate
|Restrictions||For Research Use only|
HandlingProduct Details anti-FLT3 Antibody Target Details FLT3 Application Details References for anti-FLT3 antibody (ABIN1996829) Images back to top
|Buffer||0.2 μm filtered solution in PBS with 5 % trehalose|
|Handling Advice||Avoid repeated freeze-thaw cycles.|
|Storage||4 °C,-20 °C,-80 °C|
This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
|Expiry Date||12 months|
References for anti-FLT3 antibody (ABIN1996829)Product Details anti-FLT3 Antibody Target Details FLT3 Application Details Handling Images back to top
Seto: "Histone modifications." in: Methods (San Diego, Calif.), Vol. 31, Issue 1, pp. 1-2, 2003
ImagesProduct Details anti-FLT3 Antibody Target Details FLT3 Application Details Handling References for anti-FLT3 antibody (ABIN1996829) back to top