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|Antigen||Fms-Related Tyrosine Kinase 4 (FLT4) Antibodies|
|Epitope||Extracellular Domain Alternatives|
|Conjugate||This FLT4 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|2 references available|
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Product Details anti-FLT4 AntibodyTarget Details FLT4 Application Details Handling References for anti-FLT4 antibody (ABIN115161) Images
|Specificity||Detects Human VEGFR-3/FLT-4.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human.|
|Purification||Affinity Chromatography on Protein G.|
|Immunogen||Purified recombinant human Vascular Endothelial Growth Factor Receptor 3 (rh VEGFR-3/FLT-4) extracellular domain.|
Target Details FLT4Product Details anti-FLT4 Antibody Application Details Handling References for anti-FLT4 antibody (ABIN115161) Images back to top
|Alternative Name||VEGFR-3 / Flt-4 (FLT4 Antibody Abstract)|
|Background||VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk 1) and VEGFR-3 (FLT-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR-1 to -3 is almost exclusively restricted to hematopoietic precursor cells, vascular and lymphatic endothelial cells and to the monocyte/macrophage lineage. These receptors play essential roles in vasculogenesis, hematopoiesis, angiogenesis and lymphangiogenesis. The VEGFR-3 cDNA encodes a 1298 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide. Mature VEGFR-3 is composed of a 751 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 482 aa residue cytoplasmic domain. Both VEGF-C and VEGF-D have been shown to bind and activate VEGF R3 (Flt-4). The Flt-4 gene is widely expressed in the early embryo but becomes restricted to the lymphatic endothelial a latter stage of development. It is important for lymphangiogenesis.Synonyms: FLT4, Tyrosine-protein kinase receptor FLT4, VEGF Receptor 3, VEGFR3, Vascular endothelial growth factor receptor 3|
|Pathways||RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, VEGF Signaling|
Application DetailsProduct Details anti-FLT4 Antibody Target Details FLT4 Handling References for anti-FLT4 antibody (ABIN115161) Images back to top
ELISA: 0.5-1.5 μg/mL in direct ELISA, allows detection of 0.25-0.5 ng/well. Immunohistochemistry: 1-10 μg/mL. Western blot 0.5-1 μg/mL, detection limit is approximately 5 ng/lane under reducingconditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-FLT4 Antibody Target Details FLT4 Application Details References for anti-FLT4 antibody (ABIN115161) Images back to top
|Reconstitution||Restore with sterile water to a concentration of 0.1-1.0 mg/mL|
|Buffer||PBS pH 7.4 without preservatives or stabilizers|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||The lyophilized IgG is stable at 2-8 °C for one month and for one year from despatch when kept at -20 °C. When reconstituted in sterile water to a concentration of > 0.5 mg/ml the antibody is stable for six weeks at 2-8 °C or at -20 °C for longer. Aliquot to|
|Expiry Date||12 months|
References for anti-FLT4 antibody (ABIN115161)Product Details anti-FLT4 Antibody Target Details FLT4 Application Details Handling Images back to top
|Product cited in:||
Davids, Davids, Charlton, Ng, Chong, Laubscher, Dar, Hodge, Soong, Goh: "Response to a novel multitargeted tyrosine kinase inhibitor pazopanib in metastatic Merkel cell carcinoma." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 27, Issue 26, pp. e97-100, 2009
Jussila, Valtola, Partanen, Salven, Heikkilä, Matikainen, Renkonen, Kaipainen, Detmar, Tschachler, Alitalo, Alitalo: "Lymphatic endothelium and Kaposi's sarcoma spindle cells detected by antibodies against the vascular endothelial growth factor receptor-3." in: Cancer research, Vol. 58, Issue 8, pp. 1599-604, 1998
ImagesProduct Details anti-FLT4 Antibody Target Details FLT4 Application Details Handling References for anti-FLT4 antibody (ABIN115161) back to top