anti-Human Galectin 3 antibody for Immunoassay

Recommended Galectin 3 Antibody (supplied by: Log in to see )

Antigen
Lectin, Galactoside-Binding, Soluble, 3 (LGALS3) Antibodies
  • CBP 35
  • Gal-3
  • Galectin-3
  • leg3
  • galactoside-binding
  • galectin-3
  • lectin
  • soluble
  • CBP35
  • GAL3
  • GALBP
  • GALIG
  • L31
  • LGALS2
  • MAC2
  • MGC52947
  • MGC75803
  • gal-3
  • GBP
  • L-34
  • Mac-2
  • gal3
  • galectin 3
  • Galectin-3
  • lectin, galactoside-binding, soluble, 3 L homeolog
  • lectin, galactoside-binding, soluble, 3
  • lectin, galactose binding, soluble 3
  • LGALS3
  • leg3
  • lgals3.L
  • lgals3
  • Lgals3
Reactivity
Human
403
204
107
14
3
2
2
1
1
1
1
Host
Mouse
248
186
44
25
1
Clonality
Monoclonal
Conjugate
This Galectin 3 antibody is un-conjugated
39
25
18
15
13
9
7
6
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Immunoassay (IA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
393
213
185
114
89
83
45
41
30
23
14
11
6
6
4
2
2
2
1
1
1
1
1
1
1
1
1
1
Options
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Catalog No. ABIN2192045
$ 647.55
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1110956 CA DB IA ISt ELISA WB Rabbit Log in to see Polyclonal 0
-12.841685 ABIN449145 Func IA FACS IHC (fro) IHC (p) IHC WB Mouse IgG1 Log in to see B2C10 0

General

Antigen Lectin, Galactoside-Binding, Soluble, 3 (LGALS3) Antibodies
Reactivity Human
(403), (204), (107), (14), (3), (2), (2), (1), (1), (1), (1)
Host Mouse
(248), (186), (44), (25), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Galectin 3 antibody is un-conjugated
(39), (25), (18), (15), (13), (9), (7), (6), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Immunoassay (IA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(393), (213), (185), (114), (89), (83), (45), (41), (30), (23), (14), (11), (6), (6), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-Galectin 3 Antibody

Target Details Galectin 3 Application Details Handling References for anti-Galectin 3 antibody (ABIN2192045) Images
Sterility 0.2 μm filtered
Clone B2C10
Plasmids, Primers & others

Target Details Galectin 3

Product Details anti-Galectin 3 Antibody Application Details Handling References for anti-Galectin 3 antibody (ABIN2192045) Images back to top
Antigen
Alternative Name Galectin-3 (LGALS3 Antibody Abstract)
Background The monoclonal antibody B2C10 reacts with galectin-3, a 30 kDa protein. Galectin-3 is a member of the galectin family. The protein is composed of three domains: a small amino-terminal domain, a carboxyl- terminal carbohydrate recognition domain (CRD) and amino-terminal domain containing repeating elements. Galectin-3 is normally distributed in epithelia of many organs and various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up- regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins. The expression is also affected by neoplastic transformation: up-regulated in certain types of lymphomas and thyroid carcinoma, while down-regulated in other types of malignancies, such as colon, breast, ovarian and uterine carcinomas. Galectin-3 has been shown to function through both intracellular and extracellular actions. Related to its intracellular functions, galectin-3 has been identified as a component of heterogeneous nuclear ribonuclear protein (hnRNP), a factor in pre-mRNA splicing, and has been found to control cell cycle and prevent T cell apoptosis. On the other hand, this protein has also been demonstrated to function as extracellular molecule in activating various types of cells, including monocytes/macrophages, mast cells, neutrophils and lymphocytes. Galectin-3 has been shown to mediate cell-cell and cell-extracellular matrix interactions. The monoclonal antibody B2C10 inhibits the binding of 125I-labeled galectin-3 to IgE coated on microtiter plates, the galecin-3's hemagglutination activity and galectin-3-induced superoxide production by human neutrophils. This inhibitory activity of B2C10 is probably the result of its disruption of the self-association process. The epitope of the monoclonal antibody B2C10 is found within the first 45 amino acids of galectin-3. The antibody B2C10 does not react with Galecin-3C and is cross reactive with mouse galectin-3.
Pathways RTK Signaling

Application Details

Product Details anti-Galectin 3 Antibody Target Details Galectin 3 Handling References for anti-Galectin 3 antibody (ABIN2192045) Images back to top
Application Notes For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies dilutions have to be made according to the amounts of galectin-3 to be inhibited.
Restrictions For Research Use only

Handling

Product Details anti-Galectin 3 Antibody Target Details Galectin 3 Application Details References for anti-Galectin 3 antibody (ABIN2192045) Images back to top
Buffer PBS, containing 0.1 % bovine serum albumin.
Storage 4 °C
Storage Comment Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Expiry Date 12 months

References for anti-Galectin 3 antibody (ABIN2192045)

Product Details anti-Galectin 3 Antibody Target Details Galectin 3 Application Details Handling Images back to top
Product cited in:

Feilchenfeldt, Tötsch, Sheu, Robert, Spiliopoulos, Frilling, Schmid, Meier: "Expression of galectin-3 in normal and malignant thyroid tissue by quantitative PCR and immunohistochemistry." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 16, Issue 11, pp. 1117-23, 2003

Sano, Hsu, Yu, Apgar, Kuwabara, Yamanaka, Hirashima, Liu: "Human galectin-3 is a novel chemoattractant for monocytes and macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 4, pp. 2156-64, 2000

Background publications

Liu, Hsu, Zuberi, Hill, Shenhav, Kuwabara, Chen: "Modulation of functional properties of galectin-3 by monoclonal antibodies binding to the non-lectin domains." in: Biochemistry, Vol. 35, Issue 19, pp. 6073-9, 1996