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|Antigen||Growth Factor Receptor-Bound Protein 2 (GRB2) Antibodies|
|Reactivity||Cow (Bovine), Hamster, Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This GRB2 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-GRB2 AntibodyTarget Details GRB2 Application Details Handling Images
|Specificity||Recognizes the N-terminal SH3 region of rat Grb2 at 23-25kD.|
|Purification||Protein A purified|
Recombinant rat GRB2/Ash.
Type of Immunogen: Recombinant protein
Target Details GRB2Product Details anti-GRB2 Antibody Application Details Handling Images back to top
|Alternative Name||GRB2 (GRB2 Antibody Abstract)|
Name/Gene ID: GRB2
Synonyms: GRB2, Abundant SRC homology, Adapter protein GRB2, ASH, EGFRBP-GRB2, MST084, MSTP084, Protein Ash, SH2/SH3 adapter GRB2, Grb3-3, HT027, NCKAP2
|Pathways||RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation|
Application DetailsProduct Details anti-GRB2 Antibody Target Details GRB2 Handling Images back to top
Approved: IHC-P (5 μg/mL), WB (0.5 - 2 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 0.5-2 mg/mL. Detects GRB2 (23-25kD) in RIPA lysates of non-stimulated human A431 carcinoma cells. Previous lots detected GRB2 in RIPA lysates of mouse 3T3 fibroblasts, rat L6 myoblasts, Chinese hamster ovary cells and bovine brain extract.
|Restrictions||For Research Use only|
HandlingProduct Details anti-GRB2 Antibody Target Details GRB2 Application Details Images back to top
|Buffer||PBS, 50 % glycerol.|
|Handling Advice||Avoid repeat freeze-thaw cycles.|
|Storage||4 °C,-20 °C|
Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.
ImagesProduct Details anti-GRB2 Antibody Target Details GRB2 Application Details Handling back to top