anti-Horse (Equine) HER2 antibody for Immunohistochemistry

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • CD340
  • HER-2
  • HER-2/neu
  • HER2
  • MLN 19
  • NEU
  • NGL
  • TKR1
  • Erbb-2
  • Neu
  • c-erbB2
  • c-neu
  • mKIAA3023
  • wu:fv70f10
  • zgc:63601
  • erb2
  • erb-b2 receptor tyrosine kinase 2
  • ERBB2
  • Erbb2
  • erbb2
Epitope
AA 1243-1255
130
87
71
69
60
48
48
35
31
25
24
22
22
15
14
13
13
13
12
12
12
11
11
11
11
10
10
10
10
10
10
9
9
8
7
7
7
6
6
6
5
5
5
5
5
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus)
1294
404
312
30
21
9
6
5
5
4
4
3
3
3
3
3
2
2
2
2
1
1
1
Host
Rabbit
772
584
23
16
10
3
3
Clonality
Polyclonal
Conjugate
This HER2 antibody is un-conjugated
57
44
44
42
41
27
18
18
11
11
11
10
10
9
8
8
8
8
8
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
2
2
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
671
453
412
411
351
192
191
102
99
98
96
47
24
21
18
11
6
3
3
3
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN305740
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.992908 ABIN213613 IHC IHC (p) Rabbit Cytoplasmic Domain Log in to see Polyclonal 0
9.992908 ABIN461702 IHC IHC (p) Rabbit N-Term Log in to see Polyclonal 0
6.9929075 ABIN2469527 IHC Rabbit Cytoplasmic Domain Log in to see Polyclonal 0
6.9929075 ABIN2470582 IP IHC WB Rabbit IgG AA 1243-1255 Log in to see Polyclonal 0
1 ABIN1893702 IF IHC IHC (p) IP PLA WB Rabbit IgG AA 1200-1255, C-Term Log in to see Polyclonal 0
1 ABIN1893703 IF IHC IHC (p) Rabbit IgG AA 1200-1255, C-Term Log in to see Polyclonal 0
1 ABIN2467627 IHC Rabbit N-Term Log in to see Polyclonal 0

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope AA 1243-1255
(130), (87), (71), (69), (60), (48), (48), (35), (31), (25), (24), (22), (22), (15), (14), (13), (13), (13), (12), (12), (12), (11), (11), (11), (11), (10), (10), (10), (10), (10), (10), (9), (9), (8), (7), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(1294), (404), (312), (30), (21), (9), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(772), (584), (23), (16), (10), (3), (3)
Clonality
Polyclonal
Conjugate This HER2 antibody is un-conjugated
(57), (44), (44), (42), (41), (27), (18), (18), (11), (11), (11), (10), (10), (9), (8), (8), (8), (8), (8), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (2), (2), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(671), (453), (412), (411), (351), (192), (191), (102), (99), (98), (96), (47), (24), (21), (18), (11), (6), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling Images
Brand IHC-plus™
Specificity Recognizes c-erbB-2 (185kD), but not c-erbB-1(EGF-R), c-erbB-3 or c-erbB-4.
Predicted Reactivity Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Horse, Pig (100%) Elephant, Bat, Bovine (92%) Opossum (85%).
Purification Protein A purified
Immunogen A KLH-linked peptide (AENPEYLGLDVPV) corresponding to aa1243-1255 from the human c-erbB-2/HER-2/neu oncoprotein. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Horse, Pig (100%), Elephant, Bat, Bovine (92%), Opossum (85%).
Isotype IgG
Plasmids, Primers & others

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name ERBB2 / HER2 (ERBB2 Antibody Abstract)
Background Name/Gene ID: ERBB2
Subfamily: EGF Receptor
Family: Protein Kinase

Synonyms: ERBB2, C-erb B2/neu protein, CD340, HER2, Herstatin, MLN 19, HER-2, Neu, NGL, Proto-oncogene c-ErbB-2, Proto-oncogene Neu, CD340 antigen, MLN19, p185erbB2, TKR1
Gene ID 2064
UniProt P04626
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling Images back to top
Application Notes Approved: IHC, IHC-Fr, IHC-P (5 μg/mL), IP, WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 0.5-2 μg/mL detects c-erbB-2 in RIPA lysates from A431, MDA, MCF-7, LNCap, and c-erbB-2 transfected 3T3/NIH cells. Immunoprecipitation: 4 μg immunoprecipitated c-erbB-2 from 500 μg of A431, LNCap, and c-erbB- 2 transfected 3T3/NIH RIPA lysate.
Comment

Target Species of Antibody: Human

Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer 10 mM PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Images

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 1243-1255) antibody (ABIN305740) Human Breast, Carcinoma (formalin-fixed, paraffin-embedded) stained with ERBB2 antibo...
Immunohistochemistry (IHC) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 1243-1255) antibody (ABIN305740) Anti-ERBB2 / HER2 antibody IHC of human breast, carcinoma. Immunohistochemistry of fo...