anti-Human HER2 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • CD340
  • HER-2
  • HER-2/neu
  • HER2
  • MLN 19
  • NEU
  • NGL
  • TKR1
  • Erbb-2
  • Neu
  • c-erbB2
  • c-neu
  • mKIAA3023
  • wu:fv70f10
  • zgc:63601
  • erb2
  • erb-b2 receptor tyrosine kinase 2
  • ERBB2
  • Erbb2
  • erbb2
Epitope
AA 1242-1255
102
90
70
63
60
54
53
48
46
34
26
22
21
20
19
19
19
19
19
19
19
19
19
19
18
18
18
17
15
15
13
12
12
12
10
8
8
8
8
7
7
6
6
5
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1339
484
319
34
33
15
13
8
6
5
4
3
3
3
2
2
1
1
1
Host
Mouse
962
511
32
14
13
5
3
Clonality
Monoclonal
Conjugate
This HER2 antibody is un-conjugated
76
66
59
56
55
41
19
19
12
12
12
10
10
8
8
8
8
8
7
7
7
7
7
7
7
7
6
3
2
2
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
716
609
321
319
309
309
257
134
111
102
87
21
18
18
8
6
6
5
3
3
3
2
1
1
1
1
1
1
1
Options
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Catalog No. ABIN967459
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.745287 ABIN4309121 ICC IF IHC IHC (p) WB Rabbit pTyr1248 Log in to see Polyclonal 2
12.745287 ABIN4309125 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 2
12.745287 ABIN305740 IHC (fro) IHC (p) IP WB Rabbit IgG AA 1243-1255 Log in to see Polyclonal 0
12.745287 ABIN151232 IHC (p) IP IHC WB Rabbit AA 975-1025 Log in to see Polyclonal 1
12.745287 ABIN213613 IHC (p) Rabbit Cytoplasmic Domain Log in to see Polyclonal 0
12.745287 ABIN295912 ICC IHC (p) WB Chicken IgY AA 153-433 Log in to see Polyclonal 0
12.745287 ABIN4309139 FACS IF IHC (p) WB Mouse IgG1 kappa Log in to see HRB2-451 0
12.745287 ABIN725345 IF (p) IHC (p) Rabbit IgG AA 1210-1255 Log in to see Polyclonal 0
12.745287 ABIN351500 IHC (p) WB Mouse IgG1, kappa C-Term Log in to see SPM495 0
12.745287 ABIN4309126 FACS ICC IF IHC IHC (p) WB Mouse IgG2a Log in to see 4F10 0
12.745287 ABIN498415 IF IHC (p) WB Rabbit Log in to see Polyclonal 0
12.745287 ABIN4960167 IHC IHC (p) WB Mouse IgG1 Log in to see CL0268 0
12.745287 ABIN498847 IHC (p) Rabbit pTyr1248 Log in to see Polyclonal 0
12.745287 ABIN498846 IHC (p) Rabbit pTyr877 Log in to see Polyclonal 0
12.745287 ABIN498935 IHC (p) Rabbit Log in to see Polyclonal 0
12.745287 ABIN498936 IHC (p) Rabbit Log in to see Polyclonal 0
1 ABIN391947 IHC (p) WB Rabbit Ig Fraction AA 1227-1255, C-Term, Tyr1248 Log in to see Polyclonal 4
1 ABIN391945 FACS IHC (p) WB Rabbit Ig Fraction AA 21-52, N-Term Log in to see Polyclonal 4
1 ABIN4309120 IHC IHC (p) IP WB Rabbit pTyr877 Log in to see Polyclonal 1
1 ABIN4309119 ICC IF IHC IHC (p) WB Rabbit pTyr877 Log in to see Polyclonal 1

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope AA 1242-1255
(102), (90), (70), (63), (60), (54), (53), (48), (46), (34), (26), (22), (21), (20), (19), (19), (19), (19), (19), (19), (19), (19), (19), (19), (18), (18), (18), (17), (15), (15), (13), (12), (12), (12), (10), (8), (8), (8), (8), (7), (7), (6), (6), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1339), (484), (319), (34), (33), (15), (13), (8), (6), (5), (4), (3), (3), (3), (2), (2), (1), (1), (1)
Host Mouse
(962), (511), (32), (14), (13), (5), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HER2 antibody is un-conjugated
(76), (66), (59), (56), (55), (41), (19), (19), (12), (12), (12), (10), (10), (8), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (6), (3), (2), (2)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(716), (609), (321), (319), (309), (309), (257), (134), (111), (102), (87), (21), (18), (18), (8), (6), (6), (5), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling References for anti-HER2 antibody (ABIN967459) Images
Brand BD Pharmingen™
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human c-erbB-2 aa. 1242-1255
Clone 3B5
Isotype IgG1

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling References for anti-HER2 antibody (ABIN967459) Images back to top
Antigen
Alternative Name c-erbB-2 (ERBB2 Antibody Abstract)
Background C-erbB-2 (also known as HER2/neu), a 185 kDa transmembrane glycoprotein, is a member of the type 1 growth factor receptor subfamily which also includes c-erbB-3, c-erbB-4 and the epidermal growth factor receptor (EGFR), also known as c-erbB-1. Members of this receptor subfamily mediate the proliferation and differentiation of normal cells. They have a common structure consisting of an extracellular domain, a transmembrane region, and a cytoplasmic sequence. The extracellular regions contain two cysteine-rich domains, and the intracellular regions have sequence homology to known tyrosine kinases. C-erbB-2 reactivity has been detected in proximal kidney tubules, mucosal epithelium in the gastrointestinal tract, and squamous epithelium in skin. Most normal adult tissues show little or no reactivity with antibodies against c-erbB-2. However, c-erbB-2 is overexpressed in many human breast, stomach, ovary and bladder carcinomas. EGFR, c-erbB-3 and c-erbB-4 are also overexpressed in various human tumor cells, and it is thought that aberrant activation of type 1 growth factor kinase activities may contribute to tumor progression. The antibody 3B5 recognizes human c-erbB-2. A synthetic peptide corresponding to amino acids 1242-1255 (TAENPEYLGLDVPV) in the C-terminal domain of human c-erbB-2 was used as immunogen.
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling References for anti-HER2 antibody (ABIN967459) Images back to top
Application Notes Applications include immunoprecipitation (1-2 µg/one million cells), western blot analysis (1-2 µg/ml), and immunohistochemistry of frozen and formalin-fixed paraffin-embedded tissue sections (5-20 µg/ml). Positive control cell lines include MCF7 cells (ATCC HTB 22) and SK-BR-3 (ATCC HTB 30) human breast carcinoma cells.
Comment

Related Products: ABIN968585, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details References for anti-HER2 antibody (ABIN967459) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-HER2 antibody (ABIN967459)

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling Images back to top
Product cited in:

Carraway, Cantley: "A neu acquaintance for erbB3 and erbB4: a role for receptor heterodimerization in growth signaling." in: Cell, Vol. 78, Issue 1, pp. 5-8, 1994

Martinazzi, Crivelli, Zampatti, Martinazzi: "Relationships between epidermal growth factor receptor (EGF-R) and other predictors of prognosis in breast carcinomas. An immunohistochemical study." in: Pathologica, Vol. 85, Issue 1100, pp. 637-44, 1994

Penault-Llorca, Adelaïde, Houvenaeghel, Hassoun, Birnbaum, Jacquemier: "Optimization of immunohistochemical detection of ERBB2 in human breast cancer: impact of fixation." in: The Journal of pathology, Vol. 173, Issue 1, pp. 65-75, 1994

Schwechheimer, Läufle, Schmahl, Knödlseder, Fischer, Höfler: "Expression of neu/c-erbB-2 in human brain tumors." in: Human pathology, Vol. 25, Issue 8, pp. 772-80, 1994

Singleton, Niehans, Gu, Litz, Hagen, Qiu, Kiang, Strickler: "Detection of c-erbB-2 activation in paraffin-embedded tissue by immunohistochemistry." in: Human pathology, Vol. 23, Issue 10, pp. 1141-50, 1992

Zhau, Zhang, von Eschenbach, Scorsone, Babaian, Ro, Hung: "Amplification and expression of the c-erb B-2/neu proto-oncogene in human bladder cancer." in: Molecular carcinogenesis, Vol. 3, Issue 5, pp. 254-7, 1991

van de Vijver, Peterse, Mooi, Wisman, Lomans, Dalesio, Nusse: "Neu-protein overexpression in breast cancer. Association with comedo-type ductal carcinoma in situ and limited prognostic value in stage II breast cancer." in: The New England journal of medicine, Vol. 319, Issue 19, pp. 1239-45, 1988

Background publications

Hancock, Langton, Chan, Toy, Monahan, Mischak, Shawver: "A monoclonal antibody against the c-erbB-2 protein enhances the cytotoxicity of cis-diamminedichloroplatinum against human breast and ovarian tumor cell lines." in: Cancer research, Vol. 51, Issue 17, pp. 4575-80, 1991

Meissner, Rivière, Haupt, Löning: "Study of neu-protein expression in mammary Paget's disease with and without underlying breast carcinoma and in extramammary Paget's disease." in: The American journal of pathology, Vol. 137, Issue 6, pp. 1305-9, 1991

Images

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling References for anti-HER2 antibody (ABIN967459) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 1242-1255) antibody (ABIN967459) Immunohistochemical staining for c-erbB-2. Formalin-fixed, paraffin-embedded tissue s...
 image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 1242-1255) antibody (ABIN967459) anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 1242-1255) antibody (Image 2)