anti-Monkey HER2 antibody for Immunohistochemistry (Frozen Sections)

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • CD340
  • HER-2
  • HER-2/neu
  • HER2
  • MLN 19
  • NEU
  • NGL
  • TKR1
  • Erbb-2
  • Neu
  • c-erbB2
  • c-neu
  • mKIAA3023
  • wu:fv70f10
  • zgc:63601
  • erb2
  • erb-b2 receptor tyrosine kinase 2
  • ERBB2
  • Erbb2
  • erbb2
Epitope
AA 1243-1255
128
79
70
68
59
49
47
30
30
27
25
22
21
16
15
15
13
13
12
12
11
11
11
11
11
11
11
11
11
11
11
11
10
8
8
7
7
7
6
6
5
5
5
5
5
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus)
1268
396
293
27
20
7
4
4
3
3
3
3
3
2
1
1
1
Host
Rabbit
773
564
30
14
11
3
3
Clonality
Polyclonal
Conjugate
This HER2 antibody is un-conjugated
59
45
44
42
41
27
19
19
13
12
12
12
10
10
8
8
8
8
8
7
7
7
7
7
7
7
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
3
2
2
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
645
465
410
383
332
201
197
102
102
100
80
47
24
21
18
10
6
4
3
3
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN305740
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN372478 IHC (fro) IHC (p) IP WB Rabbit IgG AA 1243-1255 Log in to see Polyclonal 0
1 ABIN959522 IHC IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see 3B5 0
1 ABIN1734112 IHC (fro) IHC (p) IP WB Rabbit IgG AA 1243-1255 Log in to see Polyclonal 0
1 ABIN2962674 FACS IHC (fro) IHC (p) IP WB Mouse IgG1 C-Term Log in to see 3B5 0
1 ABIN5541738 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 AA 1242-1255, C-Term Log in to see 3B5 0

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope AA 1243-1255
(128), (79), (70), (68), (59), (49), (47), (30), (30), (27), (25), (22), (21), (16), (15), (15), (13), (13), (12), (12), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (10), (8), (8), (7), (7), (7), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(1268), (396), (293), (27), (20), (7), (4), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1)
Host Rabbit
(773), (564), (30), (14), (11), (3), (3)
Clonality
Polyclonal
Conjugate This HER2 antibody is un-conjugated
(59), (45), (44), (42), (41), (27), (19), (19), (13), (12), (12), (12), (10), (10), (8), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (3), (2), (2), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(645), (465), (410), (383), (332), (201), (197), (102), (102), (100), (80), (47), (24), (21), (18), (10), (6), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling Images
Brand IHC-plus™
Specificity Recognizes c-erbB-2 (185kD), but not c-erbB-1(EGF-R), c-erbB-3 or c-erbB-4.
Predicted Reactivity Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Horse, Pig (100%) Elephant, Bat, Bovine (92%) Opossum (85%).
Purification Protein A purified
Immunogen A KLH-linked peptide (AENPEYLGLDVPV) corresponding to aa1243-1255 from the human c-erbB-2/HER-2/neu oncoprotein. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Horse, Pig (100%), Elephant, Bat, Bovine (92%), Opossum (85%).
Isotype IgG
Plasmids, Primers & others

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name ERBB2 / HER2 (ERBB2 Antibody Abstract)
Background Name/Gene ID: ERBB2
Subfamily: EGF Receptor
Family: Protein Kinase

Synonyms: ERBB2, C-erb B2/neu protein, CD340, HER2, Herstatin, MLN 19, HER-2, Neu, NGL, Proto-oncogene c-ErbB-2, Proto-oncogene Neu, CD340 antigen, MLN19, p185erbB2, TKR1
Gene ID 2064
UniProt P04626
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling Images back to top
Application Notes Approved: IHC, IHC-Fr, IHC-P (5 μg/mL), IP, WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 0.5-2 μg/mL detects c-erbB-2 in RIPA lysates from A431, MDA, MCF-7, LNCap, and c-erbB-2 transfected 3T3/NIH cells. Immunoprecipitation: 4 μg immunoprecipitated c-erbB-2 from 500 μg of A431, LNCap, and c-erbB- 2 transfected 3T3/NIH RIPA lysate.
Comment

Target Species of Antibody: Human

Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer 10 mM PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Images

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 1243-1255) antibody (ABIN305740) Human Breast, Carcinoma (formalin-fixed, paraffin-embedded) stained with ERBB2 antibo...