anti-Rat (Rattus) HER2 antibody for ELISA

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • CD340
  • HER-2
  • HER-2/neu
  • HER2
  • MLN 19
  • NEU
  • NGL
  • TKR1
  • Erbb-2
  • Neu
  • c-erbB2
  • c-neu
  • mKIAA3023
  • wu:fv70f10
  • zgc:63601
  • erb2
  • erb-b2 receptor tyrosine kinase 2
  • ERBB2
  • Erbb2
  • erbb2
Epitope
pTyr877
130
86
68
67
58
43
31
29
27
25
24
22
22
15
14
13
13
12
12
11
11
11
11
10
10
10
10
10
10
10
9
9
8
7
7
7
6
6
5
5
5
5
5
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1235
383
295
31
21
10
6
6
6
4
4
3
3
3
3
3
2
2
2
2
1
1
1
Host
Rabbit
730
569
22
16
8
3
3
Clonality
Polyclonal
Conjugate
This HER2 antibody is un-conjugated
55
44
42
41
40
27
16
16
11
10
10
9
9
8
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
5
2
2
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
637
450
403
399
341
189
187
96
95
82
80
47
24
21
18
8
6
3
3
3
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN6256054
$ 390.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
19 ABIN6256630 ELISA ICC IF IHC WB Rabbit IgG pTyr1248 Log in to see Polyclonal 0
19 ABIN6255774 ELISA ICC IF IHC WB Rabbit IgG pTyr1112 Log in to see Polyclonal 0
16 ABIN6271262 ELISA ICC IF IHC WB Rabbit IgG pThr686 Log in to see Polyclonal 0
16 ABIN1847482 IHC ELISA WB Rabbit pTyr1248 Log in to see Polyclonal 0
13.252602 ABIN2141046 IF ELISA Rabbit IgG pThr686 Log in to see Polyclonal 0
13.252602 ABIN2141056 ELISA WB Rabbit IgG pTyr1112 Log in to see Polyclonal 0
13 ABIN6269773 ELISA IHC WB Rabbit IgG pTyr1005 Log in to see Polyclonal 0
13 ABIN6262206 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
13 ABIN1847484 IP IHC ELISA WB Rabbit pTyr877 Log in to see Polyclonal 0
10 ABIN1534423 IF IHC ELISA WB Rabbit IgG AA 641-690 Log in to see Polyclonal 1
10 ABIN6269777 ELISA IHC WB Rabbit IgG pTyr735 Log in to see Polyclonal 0
10 ABIN6269779 ELISA IHC WB Rabbit IgG pTyr1023 Log in to see Polyclonal 0
10 ABIN2889975 ELISA IF IHC IHC (p) WB Rabbit IgG AA 641-690 Log in to see Polyclonal 0
10 ABIN6262207 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
10 ABIN1576969 IHC ELISA WB Rabbit IgG pTyr1248 Log in to see Polyclonal 0
10 ABIN1576968 IF IHC ELISA WB Rabbit Log in to see Polyclonal 0
7 ABIN1532623 IHC ELISA WB Rabbit IgG AA 661-710 Log in to see Polyclonal 2
7 ABIN1532892 IHC ELISA WB Rabbit IgG AA 851-900 Log in to see Polyclonal 0
7 ABIN1531859 ELISA IHC IP WB Rabbit IgG AA 1191-1240, pTyr1221 Log in to see Polyclonal 0
7 ABIN1531861 ELISA IHC IP WB Rabbit IgG AA 851-900, pTyr877 Log in to see Polyclonal 0

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope pTyr877
(130), (86), (68), (67), (58), (43), (31), (29), (27), (25), (24), (22), (22), (15), (14), (13), (13), (12), (12), (11), (11), (11), (11), (10), (10), (10), (10), (10), (10), (10), (9), (9), (8), (7), (7), (7), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1235), (383), (295), (31), (21), (10), (6), (6), (6), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(730), (569), (22), (16), (8), (3), (3)
Clonality
Polyclonal
Conjugate This HER2 antibody is un-conjugated
(55), (44), (42), (41), (40), (27), (16), (16), (11), (10), (10), (9), (9), (8), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (2), (2), (1)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(637), (450), (403), (399), (341), (189), (187), (96), (95), (82), (80), (47), (24), (21), (18), (8), (6), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling Images
Specificity Phospho-HER2 (Tyr877) Antibody detects endogenous levels of HER2 only when phosphorylated at Tyrosine 877
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen A synthesized peptide derived from human HER2 around the phosphorylation site of Tyrosine 877
Isotype IgG
Plasmids, Primers & others

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HER2 (ERBB2 Antibody Abstract)
Background

Description: Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.

Gene: ERBB2

Molecular Weight 185kDa
Gene ID 2064
UniProt P04626
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling Images back to top
Application Notes WB 1:500-1:2000 IHC 1:50-1:200 IP 1:100-1:500, IF/ICC 1:100-1:500
Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

Images

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr877) antibody (ABIN6256054) Western blot analysis of Phospho-HER2 (Tyr877) expression in various lysates
Immunohistochemistry (IHC) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr877) antibody (ABIN6256054) ABIN6267284 at 1/100 staining mouse gastric tissue sections by IHC-P. The tissue was ...
Immunohistochemistry (IHC) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr877) antibody (ABIN6256054) ABIN6267284 at 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was f...
Immunohistochemistry (IHC) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr877) antibody (ABIN6256054) ABIN6267284 at 1/100 staining mouse testicular tissue sections by IHC-P. The tissue w...
Immunohistochemistry (IHC) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr877) antibody (ABIN6256054) ABIN6267284 at 1/100 staining human pancreatic tissue sections by IHC-P. The tissue w...
Immunohistochemistry (IHC) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr877) antibody (ABIN6256054) ABIN6267284 at 1/100 staining human heart tissue sections by IHC-P. The tissue was fo...
Immunohistochemistry (IHC) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr877) antibody (ABIN6256054) ABIN6267284 at 1/100 staining human tongue cancer tissue sections by IHC-P. The tissu...
Immunohistochemistry (IHC) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr877) antibody (ABIN6256054) ABIN6267284 at 1/100 staining rat uterine cancer tissue sections by IHC-P. The tissue...
Immunohistochemistry (IHC) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr877) antibody (ABIN6256054) ABIN6267284 at 1/100 staining rat gastric cancer tissue sections by IHC-P. The tissue...
Immunohistochemistry (IHC) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr877) antibody (ABIN6256054) ABIN6267284 at 1/100 staining rat ovarian cancer tissue sections by IHC-P. The tissue...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr877) antibody (ABIN6256054) ABIN6267284 staining MDA-MB-231 by IF/ICC. The sample were fixed with PFA and permeab...
Western Blotting (WB) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr877) antibody (ABIN6256054) Western blot analysis of HER2 phosphorylation expression in EGF treated MDA-MB-231 wh...