anti-Human HGF antibody for ELISA

Recommended HGF Antibody (supplied by: Log in to see )

Antigen
Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) Antibodies
  • DFNB39
  • F-TCF
  • HGFB
  • HPTA
  • SF
  • HGF
  • C230052L06Rik
  • HGF/SF
  • NK1
  • NK2
  • SF/HGF
  • hepatocyte growth factor
  • HGF
  • Hgf
Epitope
AA 1-728
23
19
16
15
14
14
8
7
6
5
5
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
231
56
52
14
5
5
5
4
4
2
2
2
1
1
1
Host
Rabbit
144
109
4
4
Clonality
Polyclonal
Conjugate
This HGF antibody is un-conjugated
19
14
7
5
5
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
212
124
74
30
17
14
13
7
6
6
5
5
2
2
1
1
1
1
1
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Catalog No. ABIN1996875
$ 218.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1996879 IHC (p) ELISA Rabbit IgG AA 1-728 Log in to see Polyclonal 0
1 ABIN1584562 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN2458833 ELISA WB Rabbit Log in to see Polyclonal 0
1 ABIN5662022 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 495-728 Log in to see 0
1 ABIN251141 ELISA IHC WB Mouse IgG1 Log in to see SBF5 C1-7 1
1 ABIN1996867 ELISA Mouse IgG1 AA 1-728 Log in to see 04 0
1 ABIN5693598 FACS IHC (p) ELISA WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN1996877 ELISA Rabbit IgG AA 1-728 Log in to see Polyclonal 1
1 ABIN1996871 ELISA Rabbit IgG AA 1-728 Log in to see Polyclonal 1
1 ABIN561254 ELISA WB Mouse AA 619-728, partial Log in to see Polyclonal 1
1 ABIN1996873 ELISA Biotin Rabbit IgG AA 1-728 Log in to see Polyclonal 1
1 ABIN1996869 ELISA Rabbit IgG AA 1-728 Log in to see 007 1
1 ABIN3031184 ELISA WB Mouse IgG1 AA 521-554 Log in to see 489CT6-12-6 0
1 ABIN1901844 ELISA FACS IHC WB Biotin Rabbit IgG AA 529-559 Log in to see Polyclonal 0
1 ABIN1901842 ELISA FACS IHC WB Alkaline Phosphatase (AP) Rabbit IgG AA 529-559 Log in to see Polyclonal 0
1 ABIN1901845 ELISA FACS IHC WB FITC Rabbit IgG AA 529-559 Log in to see Polyclonal 0
1 ABIN3188093 ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN265802 ELISA WB Mouse IgG1 Log in to see 24612111 0
1 ABIN2621836 ELISA WB FITC Mouse IgG AA 495-728 Log in to see 0
1 ABIN1901841 ELISA FACS IHC WB HRP Rabbit IgG AA 529-559 Log in to see Polyclonal 0

General

Antigen Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) Antibodies
Epitope AA 1-728
(23), (19), (16), (15), (14), (14), (8), (7), (6), (5), (5), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(231), (56), (52), (14), (5), (5), (5), (4), (4), (2), (2), (2), (1), (1), (1)
Host Rabbit
(144), (109), (4), (4)
Clonality
Polyclonal
Conjugate This HGF antibody is un-conjugated
(19), (14), (7), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(212), (124), (74), (30), (17), (14), (13), (7), (6), (6), (5), (5), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-HGF Antibody

Target Details HGF Application Details Handling References for anti-HGF antibody (ABIN1996875) Images
Specificity Human HGF
Characteristics Produced in rabbits immunized with purified, recombinant Human HGF (rh HGF, ABIN2215697 NP_000592.3, Met 1-Ser 728,. HGF specific IgG was purified by Human HGF affinity chromatography.
Purification purified by affinity chromatography
Sterility 0.2 μm filtered
Immunogen Recombinant Human HGF protein (ABIN2215697)
Isotype IgG

Target Details HGF

Product Details anti-HGF Antibody Application Details Handling References for anti-HGF antibody (ABIN1996875) Images back to top
Antigen
Alternative Name HGF (HGF Antibody Abstract)
Background Hepatocyte growth factor, also known as HGF, contains 4 kringle domains, 1 PAN domain and 1 peptidase S1 domain. It belongs to the peptidase S1 family, plasminogen subfamily. Hepatocyte growth factor is secreted by mesenchymal cellsas a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor, and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as growth factor for a broad spectrum of tissues and cell types. HGF has no detectable protease activity. Defects in hepatocyte growth factor are the cause of deafness autosomal recessive type 39. A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
NCBI Accession NP_000592.3
Pathways RTK Signaling, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-HGF Antibody Target Details HGF Handling References for anti-HGF antibody (ABIN1996875) Images back to top
Application Notes ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human HGF. The detection limit for Human HGF is approximately 0.00245 ng/well.
IHC-P: 1-10 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-HGF Antibody Target Details HGF Application Details References for anti-HGF antibody (ABIN1996875) Images back to top
Format Liquid
Buffer 0.2 μm filtered solution in PBS with 5 % trehalose
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C,-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Expiry Date 12 months

References for anti-HGF antibody (ABIN1996875)

Product Details anti-HGF Antibody Target Details HGF Application Details Handling Images back to top
Background publications

Comoglio: "Structure, biosynthesis and biochemical properties of the HGF receptor in normal and malignant cells." in: EXS, Vol. 65, pp. 131-65, 1993

Images

Product Details anti-HGF Antibody Target Details HGF Application Details Handling References for anti-HGF antibody (ABIN1996875) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF) (AA 1-728) antibody (ABIN1996875) Immunochemical staining of human HGF in human cirrhosis with rabbit polyclonal antibo...