anti-Mouse (Murine) IGF1R antibody for Flow Cytometry

Recommended IGF1R Antibody (supplied by: Log in to see )

Antigen
Insulin-Like Growth Factor 1 Receptor (IGF1R) Antibodies
  • CD221
  • IGFIR
  • IGFR
  • JTK13
  • IGFIRC
  • IGFR1
  • IGF-1R
  • IGF1R
  • DKFZp469I0618
  • igf-1r
  • xIGF-1R
  • xIGFR
  • xigf1r
  • A330103N21Rik
  • D930020L01
  • hyft
  • igf-ir
  • IGF-IRa
  • sb:eu1090
  • wu:fi20b10
  • insulin like growth factor 1 receptor
  • insulin-like growth factor 1 receptor
  • insulin-like growth factor I receptor
  • insulin like growth factor 1 receptor S homeolog
  • insulin-like growth factor 1a receptor
  • IGF1R
  • Igf1r
  • igf1r
  • LOC780503
  • LOC100136427
  • igf1r.S
  • igf1ra
Epitope
AA 260-290
41
40
36
29
24
21
20
17
16
15
15
15
15
15
9
8
8
8
7
7
5
5
5
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
493
273
254
14
13
13
12
11
3
3
3
2
2
2
1
Host
Rabbit
388
106
2
2
Clonality
Polyclonal
Conjugate
This IGF1R antibody is un-conjugated
19
18
17
16
9
9
8
8
8
8
8
8
8
8
8
7
6
2
1
Application
Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
327
156
131
103
84
77
54
44
14
14
8
6
5
2
2
2
1
1
1
1
Options
Supplier
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Available images

Catalog No. ABIN726575
$ 318.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.755236 ABIN1387293 FACS IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal 2
13.755236 ABIN6244670 ELISA (Capture) FACS IHC Neut WB Mouse IgG1 AA 31-932 Log in to see 33255 0
13.755236 ABIN6244671 ELISA (Capture) FACS IHC Neut WB Mouse IgG1 AA 31-932 Log in to see 33255 0
1 ABIN901900 FACS IF (p) Alexa Fluor 647 Rabbit IgG AA 260-290 Log in to see Polyclonal 0
1 ABIN901898 FACS IF (p) Alexa Fluor 350 Rabbit IgG AA 260-290 Log in to see Polyclonal 0
1 ABIN726578 FACS IF (p) Cy3 Rabbit IgG AA 260-290 Log in to see Polyclonal 0
1 ABIN726582 FACS IF (p) FITC Rabbit IgG AA 260-290 Log in to see Polyclonal 0
1 ABIN726581 FACS IF (p) Cy7 Rabbit IgG AA 260-290 Log in to see Polyclonal 0
1 ABIN901902 FACS IF (p) Alexa Fluor 488 Rabbit IgG AA 260-290 Log in to see Polyclonal 0
1 ABIN2690172 FACS PE Rabbit IgG AA 260-290 Log in to see Polyclonal 0
1 ABIN726580 FACS IF (p) Cy5.5 Rabbit IgG AA 260-290 Log in to see Polyclonal 0
1 ABIN901899 FACS IF (p) Alexa Fluor 555 Rabbit IgG AA 260-290 Log in to see Polyclonal 0
1 ABIN2690173 FACS PE Rabbit IgG AA 850-900 Log in to see Polyclonal 0
1 ABIN726579 FACS IF (p) Cy5 Rabbit IgG AA 260-290 Log in to see Polyclonal 0
1 ABIN2808728 FACS IF (p) Alexa Fluor 594 Rabbit IgG AA 260-290 Log in to see Polyclonal 0
1 ABIN2808729 FACS IF (p) Alexa Fluor 594 Rabbit IgG AA 850-900 Log in to see Polyclonal 0
1 ABIN1397228 FACS IF (p) Alexa Fluor 350 Rabbit IgG Log in to see Polyclonal 0
1 ABIN1397231 FACS IF (p) Alexa Fluor 647 Rabbit IgG Log in to see Polyclonal 0
1 ABIN1397230 FACS IF (p) Alexa Fluor 555 Rabbit IgG Log in to see Polyclonal 0
1 ABIN1415105 FACS IF (p) Cy5 Rabbit IgG Log in to see Polyclonal 0

General

Antigen Insulin-Like Growth Factor 1 Receptor (IGF1R) Antibodies
Epitope AA 260-290
(41), (40), (36), (29), (24), (21), (20), (17), (16), (15), (15), (15), (15), (15), (9), (8), (8), (8), (7), (7), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(493), (273), (254), (14), (13), (13), (12), (11), (3), (3), (3), (2), (2), (2), (1)
Host Rabbit
(388), (106), (2), (2)
Clonality
Polyclonal
Conjugate This IGF1R antibody is un-conjugated
(19), (18), (17), (16), (9), (9), (8), (8), (8), (8), (8), (8), (8), (8), (8), (7), (6), (2), (1)
Application Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(327), (156), (131), (103), (84), (77), (54), (44), (14), (14), (8), (6), (5), (2), (2), (2), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-IGF1R Antibody

Target Details IGF1R Application Details Handling References for anti-IGF1R antibody (ABIN726575) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human IGF-1R alpha chain
Isotype IgG
Plasmids, Primers & others

Target Details IGF1R

Product Details anti-IGF1R Antibody Application Details Handling References for anti-IGF1R antibody (ABIN726575) Images back to top
Antigen
Alternative Name IGF1R (IGF1R Antibody Abstract)
Background

When present in a hybrid receptor with INSR, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.

Subcellular location: Extracellular

Synonyms: IGFR, CD221, IGFIR, JTK13, Insulin-like growth factor 1 receptor, Insulin-like growth factor I receptor, IGF-I receptor, IGF1R

Gene ID 3480
UniProt P08069
Pathways RTK Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy

Application Details

Product Details anti-IGF1R Antibody Target Details IGF1R Handling References for anti-IGF1R antibody (ABIN726575) Images back to top
Application Notes WB 1:100-1000
FCM 1:20-100
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-IGF1R Antibody Target Details IGF1R Application Details References for anti-IGF1R antibody (ABIN726575) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-IGF1R antibody (ABIN726575)

Product Details anti-IGF1R Antibody Target Details IGF1R Application Details Handling Images back to top
Product cited in:

Hu, Li, Hu, Wu, Li, Meng: "MicroRNA let-7a and let-7f as novel regulatory factors of the sika deer (Cervus nippon) IGF-1R gene." in: Growth factors (Chur, Switzerland), Vol. 32, Issue 1, pp. 27-33, 2014 Method employed by authors: Western Blotting (WB)

Yang, Liu, Li, Wen, Zhu, Xu: "Insulin-Like Growth Factor-1 Modulates Polycomb Cbx8 Expression and Inhibits Colon Cancer Cell Apoptosis." in: Cell biochemistry and biophysics, 2014 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Images

Product Details anti-IGF1R Antibody Target Details IGF1R Application Details Handling References for anti-IGF1R antibody (ABIN726575) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (AA 260-290) antibody (ABIN726575) Formalin-fixed and paraffin-embedded human lung carcinoma tissue labeled with Rabbit ...
Immunohistochemistry (IHC) image for anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (AA 260-290) antibody (ABIN726575) Formalin-fixed and paraffin embedded: human lung carcinoma labeled with Anti-IGF1R/CD...
Flow Cytometry (FACS) image for anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (AA 260-290) antibody (ABIN726575) Mouse splenocytes probed with Rabbit Anti-IGF1R Polyclonal Antibody .
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (AA 260-290) antibody (ABIN726575) Formalin-fixed and paraffin embedded: human lung carcinoma labeled with Anti-IGF1R/CD...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (AA 260-290) antibody (ABIN726575) Formalin-fixed and paraffin-embedded human lung carcinoma tissue labeled with Rabbit ...
'Independent Validation' Badge
Lot Number 130322
Method validated Western Blotting
Positive Control MCF-7 cells – high expression
Negative Control PC3 cells – low expression
Notes Two strong bands were observed in the positive control, one at the expected molecular weight, and one slightly higher (which may represent a glycosylated or precursor form). Weaker bands of the same molecular weight appear in the negative control, which is consistent with the expected lower expression in PC3 cells. No other major bands are present in either positive or negative controls.
Primary Antibody
  • Antigen: Insulin-Like Growth Factor 1 Receptor (IGF1R) (1:200 dilution)
  • Catalog number: ABIN726575
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 130322
Secondary Antibody
  • Antibody: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate (1:20,000 dilution)
  • Supplier: Log in to see
  • Catalog number: Log in to see
  • Lot number: L170-6515
Controls
  • Positive control: MCF-7 cell extract
  • Negative control: PC-3 cell extract
Protocol
  • Total protein extracts were boiled in 1X SDS Sample Buffer containing 1% SDS and 1.25% β-mercaptoethanol at 95°C for 5 min prior to loading.
  • 46 μg of boiled extracts were loaded and resolved on a 8-16% SDS-polyacrylamide gel.
  • The Spectra Multicolor Broad Range (Thermo Scientific, Cat # 26634) were used as molecular mass markers.
  • Proteins were transferred onto PVDF membrane by wet transfer and protein transfer was confirmed with Ponceau-S staining.
  • The PVDF membrane was incubated with 25 mL of blocking buffer [Tris Buffered Saline, pH 7.4 plus 0.1% TW20 (TBST)] containing 5% (W/V) non-fat dry milk at room temperature for 1 h.
  • The membrane was rinsed with TBST once.
  • The membrane was immersed with the protein side up in the primary antibody solution (anti-IGF1R; 1:200) in TBST containing 5% (W/V) non-fat dry milk and incubated for 16 hours at 4°C.
  • The membrane was rinsed in TBST three times for 5 min each.
  • The membrane was incubated in the HRP-conjugated secondary antibody solution (Goat anti-rabbit IgG-HRP; 1:20,000) in TBST containing 5% (W/V) non-fat dry milk and incubated for 1 hour at room temperature (~26°C) with gentle agitation.
  • The membrane was rinsed three times with TBST for 5 min each.
  • The membrane was rinsed in TBS twice for 30 s each.
  • Signals were detected with ECL-2 Substrate. The blot was scanned for 300 s.
  • The membrane was rinsed three times with TBST, then incubated in Acidic Glycine Stripping Buffer at room temperature with gentle agitation for 3 times, 10 min each.
  • The membrane was washed in TBST 2 times for 10 min each.
  • Repeated Steps 5-12 with the loading control antibody (anti-Actin; 1:6,000) and its matching secondary antibody (Goat anti-rabbit IgG-HRP; 1:20,000).
Experimental Notes - No challenges noted.
Validation Images
Western Blotting validation image for anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (AA 260-290) antibody (ABIN726575) Figure 1. Western blot of lysates from MCF7 cells (Lane 1) and PC-3 cells (Lane 2) pr...