anti-Non-Human Primate KIT antibody for Immunocytochemistry

Recommended KIT Antibody (supplied by: Log in to see )

Antigen
Mast/stem Cell Growth Factor Receptor (KIT) Antibodies
  • C-Kit
  • CD117
  • PBT
  • SCFR
  • Bs
  • Fdc
  • Gsfsco1
  • Gsfsco5
  • Gsfsow3
  • SCO1
  • SCO5
  • SOW3
  • Ssm
  • Tr-kit
  • W
  • c-KIT
  • c-kit
  • MGF
  • KIT
  • C-KIT
  • kit
  • receptor
  • sparse
  • Xkl-1
  • kit-a
  • kit-b
  • kl1-A
  • krk1
  • pbt
  • scfr
  • KIT proto-oncogene receptor tyrosine kinase
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog a
  • KIT proto-oncogene receptor tyrosine kinase S homeolog
  • KIT
  • Kit
  • kita
  • kit
  • kit.S
Reactivity
Human, Non-Human Primate, Cow (Bovine)
1361
538
173
45
45
37
26
19
14
4
4
4
4
3
2
1
1
1
Host
Mouse
1057
395
218
18
2
2
Clonality
Monoclonal
Conjugate
This KIT antibody is conjugated to PE
155
136
131
117
86
76
17
15
13
10
10
10
10
10
9
8
8
8
8
8
8
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
3
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
683
668
521
363
234
161
120
105
77
67
55
28
19
13
11
10
8
6
4
4
3
3
3
3
3
2
2
2
2
1
Options
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Available images

Catalog No. ABIN571351
$ 497.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
28.297794 ABIN1027690 IHC (f) ICC FACS IP APC Mouse IgG1 Log in to see 104D2 8
28.297794 ABIN1302740 FACS ICC IHC (f) IP Biotin Mouse IgG1 Log in to see 104D2 7
25.297794 ABIN638436 FACS ICC IHC (f) IP FITC Mouse IgG1 Log in to see 104D2 8
25.297794 ABIN571350 FACS ICC IHC (f) IP Mouse IgG1 Log in to see 104D2 7

General

Antigen Mast/stem Cell Growth Factor Receptor (KIT) Antibodies
Reactivity Human, Non-Human Primate, Cow (Bovine)
(1361), (538), (173), (45), (45), (37), (26), (19), (14), (4), (4), (4), (4), (3), (2), (1), (1), (1)
Host Mouse
(1057), (395), (218), (18), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This KIT antibody is conjugated to PE
(155), (136), (131), (117), (86), (76), (17), (15), (13), (10), (10), (10), (10), (10), (9), (8), (8), (8), (8), (8), (8), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
(683), (668), (521), (363), (234), (161), (120), (105), (77), (67), (55), (28), (19), (13), (11), (10), (8), (6), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1)
Pubmed 8 references available
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Product Details anti-KIT Antibody

Target Details KIT Application Details Handling References for anti-KIT antibody (ABIN571351) Images
Specificity The mouse monoclonal antibody 104D2 detects extracellular part of CD117 / c-Kit protooncogen.
HLDA VI, WS Code C-30
Cross-Reactivity (Details) Human, Non-Human Primates, Bovine
Immunogen MOLM-1 megakaryocytic cells
Clone 104D2
Isotype IgG1
Plasmids, Primers & others

Target Details KIT

Product Details anti-KIT Antibody Application Details Handling References for anti-KIT antibody (ABIN571351) Images back to top
Antigen
Alternative Name CD117 (KIT Antibody Abstract)
Background CD117 / c-Kit (stem cell factor receptor) is a 145 kDa receptor tyrosine kinase that regulates cell proliferation, adhesion, chemotaxis, apoptosis and other cell processes. Mutations of CD117 / c-Kit can lead to growth and progression of tumours. After binding of its ligand, SCF (stem cell factor), CD117 / c-Kit is autophosphorylated on its intracellular domains and activated. CD117 is expressed on pluripotent hematopoietic progenitor cells, mast cells and various cancer cells, e.g. acute myeloid leukemia cells.
Gene ID 3815
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity

Application Details

Product Details anti-KIT Antibody Target Details KIT Handling References for anti-KIT antibody (ABIN571351) Images back to top
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
Comment

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions For Research Use only

Handling

Product Details anti-KIT Antibody Target Details KIT Application Details References for anti-KIT antibody (ABIN571351) Images back to top
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

References for anti-KIT antibody (ABIN571351)

Product Details anti-KIT Antibody Target Details KIT Application Details Handling Images back to top
Product cited in:

Stevenson, McGlynn, Hodge, McLinden, George, Davies, Shiels: "Isolation, characterization, and differentiation of thy1.1-sorted pancreatic adult progenitor cell populations." in: Stem cells and development, Vol. 18, Issue 10, pp. 1389-98, 2009

Nagano, Yamashita, Hamada, Ohneda, Kimura, Nakagawa, Shibuya, Yoshikawa, Ohneda: "Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood." in: Blood, Vol. 110, Issue 1, pp. 151-60, 2007

Wihlidal, Varga, Pfeilstöcker, Karlic: "Expression and functional significance of osteocalcin splicing in disease progression of hematological malignancies." in: Leukemia research, Vol. 30, Issue 10, pp. 1241-8, 2006

Blair, Sutherland: "Primitive acute myeloid leukemia cells with long-term proliferative ability in vitro and in vivo lack surface expression of c-kit (CD117)." in: Experimental hematology, Vol. 28, Issue 6, pp. 660-71, 2000

Yoshino, Ami, Terao, Tashiro, Honda: "Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies." in: Experimental animals / Japanese Association for Laboratory Animal Science, Vol. 49, Issue 2, pp. 97-110, 2000

Broudy, Lin, Liles, Corey, OLaughlin, Mou, Linnekin: "Signaling via Src family kinases is required for normal internalization of the receptor c-Kit." in: Blood, Vol. 94, Issue 6, pp. 1979-86, 1999

Broudy, Lin, Bühring, Komatsu, Kavanagh: "Analysis of c-kit receptor dimerization by fluorescence resonance energy transfer." in: Blood, Vol. 91, Issue 3, pp. 898-906, 1998

Rappold, Ziegler, Köhler, Marchetto, Rosnet, Birnbaum, Simmons, Zannettino, Hill, Neu, Knapp, Alitalo, Alitalo, Ullrich, Kanz, Bühring: "Functional and phenotypic characterization of cord blood and bone marrow subsets expressing FLT3 (CD135) receptor tyrosine kinase." in: Blood, Vol. 90, Issue 1, pp. 111-25, 1997

Images

Product Details anti-KIT Antibody Target Details KIT Application Details Handling References for anti-KIT antibody (ABIN571351) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Mast/stem Cell Growth Factor Receptor (KIT) antibody (PE) (ABIN571351) Surface staining of human peripheral blood cells with anti-CD117 (104D2) purified.
Flow Cytometry (FACS) image for anti-Mast/stem Cell Growth Factor Receptor (KIT) antibody (PE) (ABIN571351) Surface staining of human peripheral blood cells with anti-CD117 (104D2) PE.