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|Antigen||Mast/stem Cell Growth Factor Receptor (KIT) Antibodies|
|Reactivity||Human, Non-Human Primate, Cow (Bovine) Alternatives|
|Conjugate||This KIT antibody is conjugated to PE Alternatives|
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
|8 references available|
Product Details anti-KIT AntibodyTarget Details KIT Application Details Handling References for anti-KIT antibody (ABIN571351) Images
The mouse monoclonal antibody 104D2 detects extracellular part of CD117 / c-Kit protooncogen.
HLDA VI, WS Code C-30
|Cross-Reactivity (Details)||Human, Non-Human Primates, Bovine|
|Immunogen||MOLM-1 megakaryocytic cells|
|Plasmids, Primers & others|
Target Details KITProduct Details anti-KIT Antibody Application Details Handling References for anti-KIT antibody (ABIN571351) Images back to top
|Alternative Name||CD117 (KIT Antibody Abstract)|
|Background||CD117 / c-Kit (stem cell factor receptor) is a 145 kDa receptor tyrosine kinase that regulates cell proliferation, adhesion, chemotaxis, apoptosis and other cell processes. Mutations of CD117 / c-Kit can lead to growth and progression of tumours. After binding of its ligand, SCF (stem cell factor), CD117 / c-Kit is autophosphorylated on its intracellular domains and activated. CD117 is expressed on pluripotent hematopoietic progenitor cells, mast cells and various cancer cells, e.g. acute myeloid leukemia cells.|
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity|
Application DetailsProduct Details anti-KIT Antibody Target Details KIT Handling References for anti-KIT antibody (ABIN571351) Images back to top
|Application Notes||The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.|
The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
|Restrictions||For Research Use only|
HandlingProduct Details anti-KIT Antibody Target Details KIT Application Details References for anti-KIT antibody (ABIN571351) Images back to top
|Reconstitution||No reconstitution is necessary.|
|Buffer||The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Do not freeze.
Avoid prolonged exposure to light.
|Storage Comment||Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.|
References for anti-KIT antibody (ABIN571351)Product Details anti-KIT Antibody Target Details KIT Application Details Handling Images back to top
|Product cited in:||
Stevenson, McGlynn, Hodge, McLinden, George, Davies, Shiels: "Isolation, characterization, and differentiation of thy1.1-sorted pancreatic adult progenitor cell populations." in: Stem cells and development, Vol. 18, Issue 10, pp. 1389-98, 2009
Nagano, Yamashita, Hamada, Ohneda, Kimura, Nakagawa, Shibuya, Yoshikawa, Ohneda: "Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood." in: Blood, Vol. 110, Issue 1, pp. 151-60, 2007
Wihlidal, Varga, Pfeilstöcker, Karlic: "Expression and functional significance of osteocalcin splicing in disease progression of hematological malignancies." in: Leukemia research, Vol. 30, Issue 10, pp. 1241-8, 2006
Blair, Sutherland: "Primitive acute myeloid leukemia cells with long-term proliferative ability in vitro and in vivo lack surface expression of c-kit (CD117)." in: Experimental hematology, Vol. 28, Issue 6, pp. 660-71, 2000
Yoshino, Ami, Terao, Tashiro, Honda: "Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies." in: Experimental animals / Japanese Association for Laboratory Animal Science, Vol. 49, Issue 2, pp. 97-110, 2000
Broudy, Lin, Liles, Corey, OLaughlin, Mou, Linnekin: "Signaling via Src family kinases is required for normal internalization of the receptor c-Kit." in: Blood, Vol. 94, Issue 6, pp. 1979-86, 1999
Broudy, Lin, Bühring, Komatsu, Kavanagh: "Analysis of c-kit receptor dimerization by fluorescence resonance energy transfer." in: Blood, Vol. 91, Issue 3, pp. 898-906, 1998
Rappold, Ziegler, Köhler, Marchetto, Rosnet, Birnbaum, Simmons, Zannettino, Hill, Neu, Knapp, Alitalo, Alitalo, Ullrich, Kanz, Bühring: "Functional and phenotypic characterization of cord blood and bone marrow subsets expressing FLT3 (CD135) receptor tyrosine kinase." in: Blood, Vol. 90, Issue 1, pp. 111-25, 1997
ImagesProduct Details anti-KIT Antibody Target Details KIT Application Details Handling References for anti-KIT antibody (ABIN571351) back to top