anti-Human KIT Ligand antibody for Flow Cytometry

Recommended KIT Ligand Antibody (supplied by: Log in to see )

Antigen
KIT Ligand (KITLG) Antibodies
  • Xkl-1
  • Xsl
  • Xsl-1
  • Xsl-2
  • steel
  • KITLG
  • SCF
  • kitl
  • kl-1
  • mgf
  • scf
  • FPH2
  • KL-1
  • Kitl
  • MGF
  • SF
  • SHEP7
  • Clo
  • Con
  • Gb
  • Kitlg
  • Mgf
  • SLF
  • Sl
  • blz
  • contrasted
  • 710-712
  • CSF
  • KITL
  • KIT ligand L homeolog
  • KIT ligand
  • kit ligand
  • kitlg.L
  • KITLG
  • kitlg
  • Kitl
  • Kitlg
Epitope
N-Term
18
16
9
8
7
7
5
5
5
5
4
4
3
3
3
3
3
3
2
1
1
1
1
1
Reactivity
Rat (Rattus), Mouse (Murine), Human
214
83
67
5
2
2
2
2
2
2
1
1
1
1
1
Host
Rabbit
212
61
23
1
Clonality
Polyclonal
Conjugate
This KIT Ligand antibody is un-conjugated
39
13
10
9
9
7
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Western Blotting (WB)
246
157
49
38
30
29
26
23
15
13
11
9
4
4
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN2017605
$ 257.40
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.936968 ABIN3031561 FACS IF ELISA WB Rabbit Ig Fraction AA 244-273 Log in to see Polyclonal 0
1 ABIN388293 FACS IF WB Rabbit Ig Fraction AA 244-273, C-Term Log in to see Polyclonal 0
1 ABIN5611013 EIA FACS IF WB Rabbit Ig Fraction C-Term Log in to see Polyclonal 0
1 ABIN669946 FACS PE Rabbit IgG AA 160-210 Log in to see Polyclonal 0
1 ABIN2624536 FACS WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN2356421 FACS IF ELISA WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN2356420 FACS IHC ELISA WB Rabbit IgG N-Term Log in to see Polyclonal 0
1 ABIN1908270 ELISA FACS ICC IF WB APC Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN1908272 ELISA FACS ICC IF WB PE Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN1908269 ELISA FACS ICC IF WB Alkaline Phosphatase (AP) Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN1908271 ELISA FACS ICC IF WB FITC Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN2611522 ELISA FACS IF WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN2356427 FACS IF ELISA WB PE Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN2356423 FACS IF ELISA WB APC Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN2356425 FACS IF ELISA WB FITC Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN1908268 ELISA FACS ICC IF WB HRP Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN2356424 FACS IF ELISA WB Biotin Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN2356422 FACS IF ELISA WB Alkaline Phosphatase (AP) Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN1840788 FACS IF WB Rabbit AA 244-273 Log in to see Polyclonal 0
1 ABIN4955404 ELISA FACS IF WB Rabbit Ig Fraction AA 244-273 Log in to see Polyclonal 0

General

Antigen KIT Ligand (KITLG) Antibodies
Epitope N-Term
(18), (16), (9), (8), (7), (7), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Reactivity Rat (Rattus), Mouse (Murine), Human
(214), (83), (67), (5), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(212), (61), (23), (1)
Clonality
Polyclonal
Conjugate This KIT Ligand antibody is un-conjugated
(39), (13), (10), (9), (9), (7), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Western Blotting (WB)
(246), (157), (49), (38), (30), (29), (26), (23), (15), (13), (11), (9), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-KIT Ligand Antibody

Target Details KIT Ligand Application Details Handling Images
Specificity SCF Antibody detects endogenous levels of human and mouse SCF. It is predicted to react with rat SCF protein according to sequence homology.Positive Control: Ramos cell, mouse kidney tissue lysate
Cross-Reactivity (Details) SCF Antibody detects endogenous levels of human and mouse SCF. It is predicted to react with rat SCF protein according to sequence homology.Positive Control: Ramos cell, mouse kidney tissue lysate
Purification Immunoaffinity chromatography
Immunogen KLH-coupled synthetic peptide from N-terminal of human SCF
Isotype IgG2b

Target Details KIT Ligand

Product Details anti-KIT Ligand Antibody Application Details Handling Images back to top
Antigen
Alternative Name SCF (KITLG Antibody Abstract)
Background Stem cell factor (SCF) is a growth factor that is essential for hematopoiesis, melanogenesis, and fertility. It exerts its activity at the early stages of hematopoiesis. Specifically, SCF stimulates the proliferation of mast cell and mediates cell to cell adhesion and acts synergistically with other cytokines, probably interleukins. In addition, SCF induces dimerization of c-Kit followed by trans-autophosphorylation of the cytoplasmic protein tyrosine kinase domain, leading to subsequent recruitment of signaling proteins, tyrosine phosphorylation of substrates, and activation of multiple signaling pathways.
SCF Antibody is developed in rabbit using a KLH-coupled synthetic peptide from N-terminal of human SCF (Swiss Prot: P21583).
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-KIT Ligand Antibody Target Details KIT Ligand Handling Images back to top
Application Notes Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/mLFlow cytometry: 1-3 µg for 1 x 106 cellsOther applications: user optimized
Restrictions For Research Use only

Handling

Product Details anti-KIT Ligand Antibody Target Details KIT Ligand Application Details Images back to top
Format Lyophilized
Buffer PBS, pH 7.4, containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage 4 °C/-20 °C
Storage Comment The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Images

Product Details anti-KIT Ligand Antibody Target Details KIT Ligand Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-KIT Ligand (KITLG) (N-Term) antibody (ABIN2017605) Western blot analysis of tissue lysates using SCF Antibody (ABIN399098, 1 µg/mL) The ...
Flow Cytometry (FACS) image for anti-KIT Ligand (KITLG) (N-Term) antibody (ABIN2017605) Flow cytometric analysis of Ramos cells using SCF Antibody (ABIN399098, shaded histog...