anti-Human MME antibody for ELISA (Capture)

Recommended MME Antibody (supplied by: Log in to see )

Antigen
Membrane Metallo-Endopeptidase (MME) Antibodies
  • CALLA
  • CD10
  • NEP
  • SFE
  • 6030454K05Rik
  • C85356
  • Nep
  • neprilysin
  • membrane metalloendopeptidase
  • membrane metallo endopeptidase
  • membrane metallo-endopeptidase
  • MME
  • Mme
Epitope
AA 52-750
38
29
27
16
16
11
11
10
10
8
8
8
7
6
5
5
5
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Reactivity
Human
732
120
103
16
14
11
4
4
2
2
2
2
1
1
1
1
Host
Goat
531
216
4
3
Clonality
Polyclonal
Conjugate
This MME antibody is un-conjugated
64
51
49
31
15
13
10
10
9
9
8
6
6
6
6
6
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), ELISA (Capture), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
412
337
259
186
158
122
90
50
49
21
16
13
4
4
3
2
2
2
1
1
1
1
1
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Catalog No. ABIN4900407
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General

Antigen Membrane Metallo-Endopeptidase (MME) Antibodies
Epitope AA 52-750
(38), (29), (27), (16), (16), (11), (11), (10), (10), (8), (8), (8), (7), (6), (5), (5), (5), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human
(732), (120), (103), (16), (14), (11), (4), (4), (2), (2), (2), (2), (1), (1), (1), (1)
Host Goat
(531), (216), (4), (3)
Clonality
Polyclonal
Conjugate This MME antibody is un-conjugated
(64), (51), (49), (31), (15), (13), (10), (10), (9), (9), (8), (6), (6), (6), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), ELISA (Capture), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(412), (337), (259), (186), (158), (122), (90), (50), (49), (21), (16), (13), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-MME Antibody

Target Details MME Application Details Handling References for anti-MME antibody (ABIN4900407) Images
Specificity Detects human Neprilysin/CD10 in ELISAs and Western blots. In sandwich ELISAs, approximately 20 % cross-reactivity with recombinant mouse Neprilysin is observed, and less than 0.3 % cross-reactivity with recombinant human Neprilysin-2 is observed.
Purification Immunogen affinity purified
Sterility 0.2 μm filtered
Immunogen S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Neprilysin/CD10, Tyr52-Trp750, Accession # P08473
Isotype IgG
Plasmids, Primers & others

Target Details MME

Product Details anti-MME Antibody Application Details Handling References for anti-MME antibody (ABIN4900407) Images back to top
Antigen
Alternative Name Neprilysin/CD10 (MME Antibody Abstract)
Background Alternative Names: Atriopeptidase, CALLA, CALLAmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase), CD10, CD10 antigen, CD10), CD10membrane metallo-endopeptidase variant 1, Common acute lymphocytic leukemia antigen, DKFZp686O16152, EC 3.4.24, EC 3.4.24.11, Enkephalinase, EPN, Leu-19, membrane metallo-endopeptidase, membrane metallo-endopeptidase variant 2, MGC126681, MGC126707, MME, NEPmembrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA, neprilysin, Neutral endopeptidase, Neutral Endopeptidase 24.11, NKH1, SFE, Skin fibroblast elastase
Gene ID 4311
UniProt P08473
Pathways RTK Signaling, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Smooth Muscle Cell Migration

Application Details

Product Details anti-MME Antibody Target Details MME Handling References for anti-MME antibody (ABIN4900407) Images back to top
Application Notes Western Blot 1 μg/mL
Flow Cytometry 2.5 μg/10^6 cells
Immunohistochemistry 5-15 μg/mL
Immunoprecipitation 25 μg/mL
CyTOF-ready
ELISA Capture (Matched Antibody Pair) 0.2-0.8 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-MME Antibody Target Details MME Application Details References for anti-MME antibody (ABIN4900407) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-MME antibody (ABIN4900407)

Product Details anti-MME Antibody Target Details MME Application Details Handling Images back to top
Product cited in:

Lan, Xu, Kiyota, Peng, Zheng, Ikezu: "HIV-1 reduces Abeta-degrading enzymatic activities in primary human mononuclear phagocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 12, pp. 6925-32, 2011 Method employed by authors: ELISA (ELISA), ELISA (ELISA) (Sample species: Human).

Danielyan, Schäfer, Schulz, Ladewig, Lourhmati, Buadze, Schmitt, Verleysdonk, Kabisch, Koeppen, Siegel, Proksch, Kluba, Eckert, Köhle, Schöneberg, Northoff, Schwab, Gleiter: "Survival, neuron-like differentiation and functionality of mesenchymal stem cells in neurotoxic environment: the critical role of erythropoietin." in: Cell death and differentiation, Vol. 16, Issue 12, pp. 1599-614, 2009 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Images

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Supplier Images
Immunohistochemistry (IHC) image for anti-Membrane Metallo-Endopeptidase (MME) (AA 52-750) antibody (ABIN4900407) anti-Membrane Metallo-Endopeptidase (MME) (AA 52-750) antibody
Western Blotting (WB) image for anti-Membrane Metallo-Endopeptidase (MME) (AA 52-750) antibody (ABIN4900407) anti-Membrane Metallo-Endopeptidase (MME) (AA 52-750) antibody (Image 2)