anti-Human Neuregulin 1 antibody for Cell Culture

Recommended Neuregulin 1 Antibody (supplied by: Log in to see )

Antigen
Neuregulin 1 (NRG1) Antibodies
  • 6030402G23Rik
  • ARIA
  • D230005F13Rik
  • GGF
  • GGFII
  • HRG
  • HRGalpha
  • Hgl
  • NDF
  • SMDF
  • GGF2
  • HGL
  • HRG1
  • HRGA
  • MST131
  • NRG-1
  • heregulin
  • nrg1-A
  • pro-neuregulin-1
  • NRG1
  • wu:fm70e01
  • DKFZp459A1838
  • neuregulin 1
  • neuregulin 1 L homeolog
  • Nrg1
  • NRG1
  • nrg1.L
  • nrg1
Epitope
AA 1-525
22
15
13
10
10
8
8
8
8
7
7
6
5
4
4
4
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
236
89
82
3
3
2
1
1
1
1
1
Host
Mouse
225
47
Clonality
Monoclonal
Conjugate
This Neuregulin 1 antibody is un-conjugated
21
13
7
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Cell Culture (CC), ELISA
227
98
97
44
23
18
16
13
11
8
5
5
5
4
2
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Catalog No. ABIN1997617
$ 218.90
Plus shipping costs $45.00

General

Antigen Neuregulin 1 (NRG1) Antibodies
Epitope AA 1-525
(22), (15), (13), (10), (10), (8), (8), (8), (8), (7), (7), (6), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(236), (89), (82), (3), (3), (2), (1), (1), (1), (1), (1)
Host Mouse
(225), (47)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Neuregulin 1 antibody is un-conjugated
(21), (13), (7), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cell Culture (CC), ELISA
(227), (98), (97), (44), (23), (18), (16), (13), (11), (8), (5), (5), (5), (4), (2)
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Product Details anti-Neuregulin 1 Antibody

Target Details Neuregulin 1 Application Details Handling Images
Specificity Human HPRG / HRG
No cross-reactivity with Human cell lysate (293 cell line) in WB and ELISA.
No Cross-Reactivity Human
Characteristics This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human HPRG (rhHPRG, ABIN2003464 Met 1-Lys 525, NP_000403.1,. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Purification purified by Protein A affinity chromatography
Sterility 0.2 μm filtered
Immunogen Recombinant Human HPRG protein (ABIN2003464)
Clone 2D9D4
Isotype IgG1
Plasmids, Primers & others

Target Details Neuregulin 1

Product Details anti-Neuregulin 1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HRG (NRG1 Antibody Abstract)
Background Histidine-rich glycoprotein, also known as HRG and HPRG, is a glycoprotein located in plasma and platelets, and contains an unusually large amount of histidine and proline. In human, five distinct domains are recognized in the mature HPRG molecule. There are two N-terminal cystatin- like modules (aa 19 - 254) and one His-Pro-rich region (aa 350 - 497) that is flanked by two Pro-rich segments (aa 276 - 321 and 498 - 525). The His-Pro-rich region contains 10 tandem repeats with an HHPHG motif, and the N- and C-termini are linked by a disulfide bond. The specific functions of HRG remain unclear, but it is known that the protein binds heme, dyes and divalent metal ions. It inhibits rosette formation and interacts with heparin, thrombospondin and plasminogen. Two of the protein's effects, the inhibition of fibrinolysis and the reduction of inhibition of coagulation, indicate a potential prothrombotic effect. HPRG is evolutionarily, functionally and structurally related to cleaved high molecular weight kininogen (HKa), an anti-angiogenic polypeptide that stimulates apoptosis of proliferating endothelial cells through binding to cell-surface tropomyosin. The antiangiogenic activity of the multidomain plasma protein HPRG is localized to its histidine-proline-rich (H/P) domain and has recently been shown to be mediated, at least partially, through binding to cell-surface tropomyosin in fibroblast growth factor-2-activated endothelial cells.
NCBI Accession NP_000403.1
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation

Application Details

Product Details anti-Neuregulin 1 Antibody Target Details Neuregulin 1 Handling Images back to top
Application Notes ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect HPRG. The detection limit for HPRG is approximately 0.16 ng /well.
ELISA(Cap): 0.5-4 μg/mL In a sandwich ELISA, it can be used as capture antibody.
Restrictions For Research Use only

Handling

Product Details anti-Neuregulin 1 Antibody Target Details Neuregulin 1 Application Details Images back to top
Format Liquid
Buffer 0.2 μm filtered solution in PBS with 5 % trehalose
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C,-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Expiry Date 12 months