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|Antigen||Neuregulin 1 (NRG1) Antibodies|
|Epitope||Extracellular Domain Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Neuregulin 1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
|4 references available|
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Product Details anti-Neuregulin 1 AntibodyTarget Details Neuregulin 1 Application Details Handling References for anti-Neuregulin 1 antibody (ABIN264425) Images
|Specificity||Recognizes a glycoprotein of 44 kDa, identified as heregulin (HRG) or neu differentiation factor (NDF) which binds to c-erbB-3 and c-erbB-4 receptors with low and high affinities, respectively. This antibody is directed against the Extracellular domain of NDF/HRG and is highly specific. It shows no cross-reaction with EGF and reacts with both alpha- and beta-isoforms of NDF/HRG, suggesting that its epitope is outside the EGF-domain. This antibody does not inhibit the binding of HRG to ErbB receptors and is excellent for multiple applications.Extracellular domain|
|Characteristics||Synonyms: NRG1, GGF, HGL, HRGA, NDF, SMDF, Heregulin, HRG, Breast cancer cell differentiation factorp45, Glial growth factor, Neu differentiation factor, Pro-neuregulin-1 membrane-boundisoform, Acetylcholine receptor-inducing activity, Sensory and motor neuron-derived factor|
|Immunogen||Hybridoma produced by the fusion of spelocytes from BALB/c mice immunized withrecombinant extracellular domain of rat NDF protein coupled to KLH and mouse myelomaNSO cells. Genename: NRG1|
Target Details Neuregulin 1Product Details anti-Neuregulin 1 Antibody Application Details Handling References for anti-Neuregulin 1 antibody (ABIN264425) Images back to top
|Alternative Name||Neuregulin 1 (NRG1 Antibody Abstract)|
|Background||HRG/NDF exists in several isoforms, which are classified in two groups alpha and beta.Synonyms: Acetylcholine receptor-inducing activity, Breast cancer cell differentiation factor p45, GGF, Glial growth factor, HGL, HRG, HRGA, Heregulin, NDF, NRG1, Neu differentiation factor, Pro-neuregulin-1 membrane-bound isoform, SMDF, Sensory and motor neuron-derived factor|
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation|
Application DetailsProduct Details anti-Neuregulin 1 Antibody Target Details Neuregulin 1 Handling References for anti-Neuregulin 1 antibody (ABIN264425) Images back to top
Western Blot (1-5 μg/mL). Immunoprecipitation (2 μg/mg of protein lysate). Immunohistochemistry on Frozen and Formalin/Paraffin Sections (1/20-1/40). Positive Control: HRGa1, HRGb1 recombinant protein or MDA-MB-231 cells, prostatecarcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Neuregulin 1 Antibody Target Details Neuregulin 1 Application Details References for anti-Neuregulin 1 antibody (ABIN264425) Images back to top
|Buffer||PBS containing 0.08 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
|Expiry Date||12 months|
References for anti-Neuregulin 1 antibody (ABIN264425)Product Details anti-Neuregulin 1 Antibody Target Details Neuregulin 1 Application Details Handling Images back to top
Beerli, Graus-Porta, Woods-Cook, Chen, Yarden, Hynes: "Neu differentiation factor activation of ErbB-3 and ErbB-4 is cell specific and displays a differential requirement for ErbB-2." in: Molecular and cellular biology, Vol. 15, Issue 12, pp. 6496-505, 1996
Karunagaran, Tzahar, Beerli, Chen, Graus-Porta, Ratzkin, Seger, Hynes, Yarden: "ErbB-2 is a common auxiliary subunit of NDF and EGF receptors: implications for breast cancer." in: The EMBO journal, Vol. 15, Issue 2, pp. 254-64, 1996
Graus-Porta, Beerli, Hynes: "Single-chain antibody-mediated intracellular retention of ErbB-2 impairs Neu differentiation factor and epidermal growth factor signaling." in: Molecular and cellular biology, Vol. 15, Issue 3, pp. 1182-91, 1995
Marte, Graus-Porta, Jeschke, Fabbro, Hynes, Taverna: "NDF/heregulin activates MAP kinase and p70/p85 S6 kinase during proliferation or differentiation of mammary epithelial cells." in: Oncogene, Vol. 10, Issue 1, pp. 167-75, 1995
ImagesProduct Details anti-Neuregulin 1 Antibody Target Details Neuregulin 1 Application Details Handling References for anti-Neuregulin 1 antibody (ABIN264425) back to top