anti-Human NFAT1 antibody for Enzyme Immunoassay

Recommended NFAT1 Antibody (supplied by: Log in to see )

Antigen
Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1) Antibodies
  • NFATC2
  • AI607462
  • NF-ATc2
  • NFAT1
  • NFAT1-D
  • Nfatp
  • NFATP
  • nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
  • nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2
  • NFATC2
  • nfatc2
  • Nfatc2
Epitope
pSer330
35
15
14
6
6
5
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
150
87
80
12
8
8
6
6
6
5
1
1
1
Host
Rabbit
109
26
19
Clonality
Polyclonal
Conjugate
This NFAT1 antibody is un-conjugated
6
6
6
4
4
4
3
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA)
92
55
39
35
21
20
19
18
16
10
4
2
2
1
1
1
Supplier
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Catalog No. ABIN358367
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5551937 EIA IHC (p) WB Goat Internal Region Log in to see Polyclonal

General

Antigen Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1) Antibodies
Epitope pSer330
(35), (15), (14), (6), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(150), (87), (80), (12), (8), (8), (6), (6), (6), (5), (1), (1), (1)
Host Rabbit
(109), (26), (19)
Clonality
Polyclonal
Conjugate This NFAT1 antibody is un-conjugated
(6), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA)
(92), (55), (39), (35), (21), (20), (19), (18), (16), (10), (4), (2), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-NFAT1 Antibody

Target Details NFAT1 Application Details Handling References for anti-NFAT1 antibody (ABIN358367) Images
Specificity This antibody detects NFATC2 pSer330.
Purification Protein A Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S330 of human NFATC2.
Isotype Ig

Target Details NFAT1

Product Details anti-NFAT1 Antibody Application Details Handling References for anti-NFAT1 antibody (ABIN358367) Images back to top
Antigen
Alternative Name NFATC2 (NFAT1 Antibody Abstract)
Background NFATC2 is a member of the nuclear factor of activated T cells (NFAT) family. It is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response.Synonyms: NFAT pre-existing subunit, NFAT-1, NFATC2, NFATP, Nuclear factor of activated T-cells, T-cell transcription factor NFAT1, cytoplasmic 2
Molecular Weight 99654 Da
Gene ID 4773, 9606
UniProt Q13469
Pathways RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, VEGF Signaling

Application Details

Product Details anti-NFAT1 Antibody Target Details NFAT1 Handling References for anti-NFAT1 antibody (ABIN358367) Images back to top
Application Notes ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-NFAT1 Antibody Target Details NFAT1 Application Details References for anti-NFAT1 antibody (ABIN358367) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-NFAT1 antibody (ABIN358367)

Product Details anti-NFAT1 Antibody Target Details NFAT1 Application Details Handling Images back to top
Background publications

Golks, Tran, Goetschy, Guerini: "Requirement for O-linked N-acetylglucosaminyltransferase in lymphocytes activation." in: The EMBO journal, Vol. 26, Issue 20, pp. 4368-79, 2007

Dong, Patino-Lopez, Candotti, Shaw: "Structure-function analysis of the WIP role in T cell receptor-stimulated NFAT activation: evidence that WIP-WASP dissociation is not required and that the WIP NH2 terminus is inhibitory." in: The Journal of biological chemistry, Vol. 282, Issue 41, pp. 30303-10, 2007

Gibson, Hedgcock, Aufiero, Wilson, Hafner, Tsokos, Wong: "Induction of the CTLA-4 gene in human lymphocytes is dependent on NFAT binding the proximal promoter." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 6, pp. 3831-40, 2007

Images

Product Details anti-NFAT1 Antibody Target Details NFAT1 Application Details Handling References for anti-NFAT1 antibody (ABIN358367) back to top
Supplier Images
 image for anti-Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1) (pSer330) antibody (ABIN358367) anti-Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1) (pSer330) antibody