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Submit your validation data for this product and get a full refund. I want to validate this productRelevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
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14.548473 | ABIN4339295 | ICC IF IHC IHC (p) | Rabbit | IgG | Log in to see | Polyclonal | 1 | |||
10 | ABIN5774624 | IF IHC (p) WB | Rabbit | IgG | Log in to see | Polyclonal | 0 | |||
5.24175 | ABIN5950481 | FACS ICC IF IHC IHC (p) WB | Rabbit | IgG | C-Term | Log in to see | JA11-08 | 0 | ||
4 | ABIN2665286 | IF | Mouse | IgG1 kappa | Log in to see | 16A12A31 | 6 | |||
1 | ABIN596783 | IF IP WB | Rabbit | N-Term | Log in to see | Polyclonal | 0 | |||
1 | ABIN5701142 | ELISA IF IHC WB | Rabbit | IgG | Log in to see | Polyclonal | 0 | |||
1 | ABIN2324631 | IF IP WB | Rabbit | Log in to see | Polyclonal | 0 | ||||
-12.75825 | ABIN4339306 | ELISA ICC IF IHC (fro) IHC (p) WB | Alexa Fluor 405 | Mouse | IgG1 | Log in to see | 25A10-D6-D2 | 0 | ||
-12.75825 | ABIN4339297 | ELISA ICC IF IHC (fro) IHC (p) WB | DyLight 350 | Mouse | IgG1 | Log in to see | 25A10-D6-D2 | 0 | ||
-12.75825 | ABIN4339302 | ELISA ICC IF IHC (fro) IHC (p) WB | DyLight 755 | Mouse | IgG1 | Log in to see | 25A10-D6-D2 | 0 | ||
-12.75825 | ABIN4339303 | ELISA ICC IF IHC (fro) IHC (p) WB | DyLight 488 | Mouse | IgG1 | Log in to see | 25A10-D6-D2 | 0 | ||
-12.75825 | ABIN4339308 | ELISA ICC IF IHC (fro) IHC (p) WB | Alexa Fluor 647 | Mouse | IgG1 | Log in to see | 25A10-D6-D2 | 0 | ||
-12.75825 | ABIN4339307 | ELISA ICC IF IHC (fro) IHC (p) WB | Alexa Fluor 488 | Mouse | IgG1 | Log in to see | 25A10-D6-D2 | 0 | ||
-12.75825 | ABIN4339309 | ELISA ICC IF IHC (fro) IHC (p) WB | Alexa Fluor 700 | Mouse | IgG1 | Log in to see | 25A10-D6-D2 | 0 | ||
-12.75825 | ABIN4339305 | ELISA ICC IF IHC (fro) IHC (p) WB | DyLight 680 | Mouse | IgG1 | Log in to see | 25A10-D6-D2 | 0 | ||
-12.75825 | ABIN4339298 | ELISA ICC IF IHC (fro) IHC (p) WB | DyLight 405 | Mouse | IgG1 | Log in to see | 25A10-D6-D2 | 0 | ||
-12.75825 | ABIN5950480 | ICC IF IHC IHC (fro) IHC (p) | Janelia Fluor® 646 | Mouse | IgG1 | Log in to see | 25A10-D6-D2 | 0 | ||
-12.75825 | ABIN5950479 | ICC IF IHC IHC (fro) IHC (p) | Janelia Fluor® 549 | Mouse | IgG1 | Log in to see | 25A10-D6-D2 | 0 |
General |
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Antigen | Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1) Antibodies |
Reactivity | Human, Mouse (Murine), Rat (Rattus) Alternatives |
Host | Mouse Alternatives |
Clonality (Clone) | |
Conjugate | This NFAT1 antibody is un-conjugated Alternatives |
Application |
Chromatin Immunoprecipitation (ChIP), ELISA, Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
Alternatives
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10 references available |
Supplier | Log in to see |
Product Details anti-NFAT1 AntibodyTarget Details NFAT1 Application Details Handling References for anti-NFAT1 antibody (ABIN152663) Images |
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Specificity | Detects nuclear factor of activated T-cells (NFAT). This does not cross react with NFAT2 (NFATc, NFATc1). |
Purification | Protein G purified |
Immunogen | Synthetic peptide corresponding to residues A(51) I S S P S G L A Y P D D V L D Y G L(69) of mouse NFATc2-A, B and C isoforms. |
Clone | 25A10-D6-D2 |
Isotype | IgG1 |
Plasmids, Primers & others | |
Target Details NFAT1Product Details anti-NFAT1 Antibody Application Details Handling References for anti-NFAT1 antibody (ABIN152663) Images back to top |
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Antigen | |
Alternative Name | NFATC2/NFAT1 (NFAT1 Antibody Abstract) |
Background | Gene Symbol: NFATC2 |
Molecular Weight | Theoretical MW: 140 kDa |
Gene ID | 4773 |
Pathways | RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, VEGF Signaling |
Application DetailsProduct Details anti-NFAT1 Antibody Target Details NFAT1 Handling References for anti-NFAT1 antibody (ABIN152663) Images back to top |
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Application Notes | Western Blot 1 μg/mL, Chromatin Immunoprecipitation 1 - 3 μL, Immunocytochemistry/Immunofluorescence 1:100 - 1:1000, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:50 - 1:2000, Immunohistochemistry-Frozen 1:50 - 1:2000, Gel Super Shift Assays 1:1 - 1:100, Sandwich ELISA 1:100 - 1:2000ChIP usage was reported in scientific literature (PMID: 15347678). In WB: Detects an approx. 140 kDa protein representing phosphorylated NFAT1 in restiing immune cell and approx. 120 kDa protein in stimulated cells that represents a fully dephosphorylated NAFT1. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Comment |
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo. |
Restrictions | For Research Use only |
HandlingProduct Details anti-NFAT1 Antibody Target Details NFAT1 Application Details References for anti-NFAT1 antibody (ABIN152663) Images back to top |
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Format | Liquid |
Concentration | 1 mg/mL |
Buffer |
PBS Buffer contains: 0.05 % Sodium Azide |
Preservative | Sodium azide |
Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Handling Advice | Avoid freeze-thaw cycles |
Storage | -20 °C |
Storage Comment | Store at -20°C. Avoid freeze-thaw cycles. |
References for anti-NFAT1 antibody (ABIN152663)Product Details anti-NFAT1 Antibody Target Details NFAT1 Application Details Handling Images back to top |
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Product cited in: |
Song, Carr, McDermott, Rodriguez, Babicheva, Balistrieri, Ayon, Wang, Makino, Yuan: "STIM2 (Stromal Interaction Molecule 2)-Mediated Increase in Resting Cytosolic Free Ca2+ Concentration Stimulates PASMC Proliferation in Pulmonary Arterial Hypertension." in: Hypertension (Dallas, Tex. : 1979), Vol. 71, Issue 3, pp. 518-529, 2018 Liu, Han, Han, He: "Mesenchymal stem cell-conditioned media suppresses inflammation-associated overproliferation of pulmonary artery smooth muscle cells in a rat model of pulmonary hypertension." in: Experimental and therapeutic medicine, Vol. 11, Issue 2, pp. 467-475, 2016 Cotroneo, Ashek, Wang, Wharton, Dubois, Bozorgi, Busbridge, Alavian, Wilkins, Zhao: "Iron homeostasis and pulmonary hypertension: iron deficiency leads to pulmonary vascular remodeling in the rat." in: Circulation research, Vol. 116, Issue 10, pp. 1680-90, 2015 (Sample species: Rat (Rattus)). Further details: Western Blotting,Immunohistochemistry Iampietro, Morissette, Gravel, Flamand: "Inhibition of interleukin-2 gene expression by human herpesvirus 6B U54 tegument protein." in: Journal of virology, Vol. 88, Issue 21, pp. 12452-63, 2014 (Sample species: Human). Further details: Western Blotting Alpini, Franchitto, Demorrow, Onori, Gaudio, Wise, Francis, Venter, Kopriva, Mancinelli, Carpino, Stagnitti, Ueno, Han, Meng, Glaser: "Activation of alpha(1) -adrenergic receptors stimulate the growth of small mouse cholangiocytes via calcium-dependent activation of nuclear factor of activated T cells 2 and specificity protein 1." in: Hepatology (Baltimore, Md.), Vol. 53, Issue 2, pp. 628-39, 2011 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry Landry, Banerjee, Taylor, Aplan, Singer, Wu: "Chromatin remodeling complex NURF regulates thymocyte maturation." in: Genes & development, Vol. 25, Issue 3, pp. 275-86, 2011 Bonnet, Rochefort, Sutendra, Archer, Haromy, Webster, Hashimoto, Bonnet, Michelakis: "The nuclear factor of activated T cells in pulmonary arterial hypertension can be therapeutically targeted." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 27, pp. 11418-23, 2007 Barlic, McDermott, Merrell, Gonzales, Via, Murphy: "Interleukin (IL)-15 and IL-2 reciprocally regulate expression of the chemokine receptor CX3CR1 through selective NFAT1- and NFAT2-dependent mechanisms." in: The Journal of biological chemistry, Vol. 279, Issue 47, pp. 48520-34, 2004 Kornau, Schenker, Kennedy, Seeburg: "Domain interaction between NMDA receptor subunits and the postsynaptic density protein PSD-95." in: Science (New York, N.Y.), Vol. 269, Issue 5231, pp. 1737-40, 1995 Younan, Iampietro, Nishida, Ramanathan, Santos, Dutta, Lubaki, Koup, Katze, Bukreyev: "Ebola Virus Binding to Tim-1 on T Lymphocytes Induces a Cytokine Storm." in: mBio, Vol. 8, Issue 5, 1970 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)). |
ImagesProduct Details anti-NFAT1 Antibody Target Details NFAT1 Application Details Handling References for anti-NFAT1 antibody (ABIN152663) back to top |
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