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|Antigen||Nuclear Factor of Activated T-Cells 5, Tonicity-Responsive (NFAT5) Antibodies|
|Reactivity||Hamster, Human, Mouse (Murine), Rabbit, Rat (Rattus) Alternatives|
|Conjugate||This NFAT5 antibody is un-conjugated Alternatives|
Chromatin Immunoprecipitation (ChIP), Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
|2 references available|
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Product Details anti-NFAT5 AntibodyTarget Details NFAT5 Application Details Handling References for anti-NFAT5 antibody (ABIN267152) Images
|Purification||Immunogen affinity purified|
|Immunogen||Synthetic peptide corresponding to residues C D(1439) L L V S L Q N Q G N N L T G S F(1455) of human NFAT5.|
Target Details NFAT5Product Details anti-NFAT5 Antibody Application Details Handling References for anti-NFAT5 antibody (ABIN267152) Images back to top
|Alternative Name||NFAT5 (NFAT5 Antibody Abstract)|
|Background||Gene Symbol: NFAT5|
|Research Area||Chromatin and Nuclear Signaling, Transcription Factors, Adaptive Immunity|
|Pathways||RTK Signaling, WNT Signaling|
Application DetailsProduct Details anti-NFAT5 Antibody Target Details NFAT5 Handling References for anti-NFAT5 antibody (ABIN267152) Images back to top
|Application Notes||Western Blot 1:1000, Chromatin Immunoprecipitation 1:10 - 1:500, Immunohistochemistry 1:20, Immunoprecipitation 3 μg, Immunohistochemistry-Paraffin 1:20, Gel Super Shift Assays 1:1 - 1:100, Immunofluorescence 1:100, Immunocytochemistry 1:1000ChIP usage was reported in scientific literature (PMID: 22266867). WB: Detects an approx. 170 kDa protein representing NFAT 5 in HEK293 cells transfected with the human NFAT 5 gene. ICC: Staining of NFAT 5 in HEK293 cells transfected with the human NFAT5 gene results in primarily cytoplasmic staining.|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
|Restrictions||For Research Use only|
HandlingProduct Details anti-NFAT5 Antibody Target Details NFAT5 Application Details References for anti-NFAT5 antibody (ABIN267152) Images back to top
PBS containing 1 mg/mL BSA
Buffer contains: 0.05 % Sodium Azide
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||Store at -20°C. Avoid freeze-thaw cycles.|
References for anti-NFAT5 antibody (ABIN267152)Product Details anti-NFAT5 Antibody Target Details NFAT5 Application Details Handling Images back to top
|Product cited in:||
Hollborn, Fischer, Kuhrt, Wiedemann, Bringmann, Kohen: "Osmotic regulation of NFAT5 expression in RPE cells: The involvement of purinergic receptor signaling." in: Molecular vision, Vol. 23, pp. 116-130, 2017 Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Human).
Johnson, Shapiro, Risbud: "RNA Sequencing Reveals a Role of TonEBP Transcription Factor in Regulation of Pro-inflammatory Genes in Response to Hyperosmolarity in Healthy Nucleus Pulposus Cells: A HOMEOSTATIC RESPONSE?" in: The Journal of biological chemistry, Vol. 291, Issue 52, pp. 26686-26697, 2016 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).
ImagesProduct Details anti-NFAT5 Antibody Target Details NFAT5 Application Details Handling References for anti-NFAT5 antibody (ABIN267152) back to top