anti-Cow (Bovine) PDGFB antibody for Western Blotting

Recommended PDGFB Antibody (supplied by: Log in to see )

Antigen
Platelet-Derived Growth Factor beta Polypeptide (PDGFB) Antibodies
  • PDGF-2
  • PDGF2
  • SIS
  • SSV
  • c-sis
  • pdgf2
  • sis
  • si:ch211-79m20.1
  • si:dkey-261d11.1
  • PDGF-B
  • Sis
  • platelet derived growth factor subunit B
  • platelet-derived growth factor beta polypeptide
  • platelet derived growth factor subunit B S homeolog
  • platelet-derived growth factor beta polypeptide a
  • platelet derived growth factor, B polypeptide
  • PDGFB
  • pdgfb.S
  • pdgfba
  • Pdgfb
Epitope
N-Term
20
18
18
15
8
6
5
3
2
2
2
2
2
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
111
85
50
2
2
2
2
Host
Rabbit
123
27
5
3
Clonality
Polyclonal
Conjugate
This PDGFB antibody is un-conjugated
9
7
5
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
129
53
34
31
18
13
8
6
6
6
3
2
2
2
2
1
1
Options
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Pubmed
1 reference available

Available images

Western Blotting (WB) image for anti-Platelet-Derived Growth Factor beta Polypeptide (PDGFB) (N-Term) antibody (ABIN2787714)
Immunohistochemistry (IHC) image for anti-Platelet-Derived Growth Factor beta Polypeptide (PDGFB) (N-Term) antibody (ABIN2787714)
Catalog No. ABIN2787714
$ 289.00
Plus shipping costs $45.00

General

Antigen Platelet-Derived Growth Factor beta Polypeptide (PDGFB) Antibodies
Epitope N-Term
(20), (18), (18), (15), (8), (6), (5), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
(111), (85), (50), (2), (2), (2), (2)
Host Rabbit
(123), (27), (5), (3)
Clonality
Polyclonal
Conjugate This PDGFB antibody is un-conjugated
(9), (7), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(129), (53), (34), (31), (18), (13), (8), (6), (6), (6), (3), (2), (2), (2), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-PDGFB Antibody

Target Details PDGFB Application Details Handling References for anti-PDGFB antibody (ABIN2787714) Images
Blocking Peptide Blocking peptide for this product available: ABIN981524
Sequence NRCWALFLSL CCYLRLVSAE GDPIPEELYE MLSDHSIRSF DDLQRLLHGD
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Sheep: 100%
Characteristics This is a rabbit polyclonal antibody against PDGFB. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human PDGFB
Plasmids, Primers & others

Target Details PDGFB

Product Details anti-PDGFB Antibody Application Details Handling References for anti-PDGFB antibody (ABIN2787714) Images back to top
Antigen
Alternative Name PDGFB (PDGFB Antibody Abstract)
Background PDGFB is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor.The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two splice variants have been identified for this gene.
Alias Symbols: FLJ12858, PDGF2, SIS, SSV, c-sis
Protein Interaction Partner: COL6A1, COL5A1, COL4A1, COL3A1, COL2A1, COL1A2, COL1A1, LYVE1, NRP1, ADIPOQ, MDFI, PDAP1, ART1, PDGFB, HRAS, THBS1, PDGFRA, PDGFRB, HSPG2, LRP1, A2M, SPARC,
Protein Size: 241
Molecular Weight 27 kDa
Gene ID 5155
NCBI Accession NM_002608, NP_002599
UniProt P01127
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling

Application Details

Product Details anti-PDGFB Antibody Target Details PDGFB Handling References for anti-PDGFB antibody (ABIN2787714) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 241 AA
Restrictions For Research Use only

Handling

Product Details anti-PDGFB Antibody Target Details PDGFB Application Details References for anti-PDGFB antibody (ABIN2787714) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-PDGFB antibody (ABIN2787714)

Product Details anti-PDGFB Antibody Target Details PDGFB Application Details Handling Images back to top
Background publications

Chadjichristos, Morel, Derouette, Sutter, Roth, Brisset, Bochaton-Piallat, Kwak: "Targeting connexin 43 prevents platelet-derived growth factor-BB-induced phenotypic change in porcine coronary artery smooth muscle cells." in: Circulation research, Vol. 102, Issue 6, pp. 653-60, 2008

Images

Product Details anti-PDGFB Antibody Target Details PDGFB Application Details Handling References for anti-PDGFB antibody (ABIN2787714) back to top
Supplier Images
Western Blotting (WB) image for anti-Platelet-Derived Growth Factor beta Polypeptide (PDGFB) (N-Term) antibody (ABIN2787714) WB Suggested Anti-PDGFB Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Immunohistochemistry (IHC) image for anti-Platelet-Derived Growth Factor beta Polypeptide (PDGFB) (N-Term) antibody (ABIN2787714) Immunohistochemistry with Uterus tissue at an antibody concentration of 5µg/ml using ...