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General |
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Antigen | Platelet-Derived Growth Factor beta Polypeptide (PDGFB) Antibodies |
Epitope | N-Term Alternatives |
Reactivity | Cow (Bovine), Dog (Canine), Guinea Pig, Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine) Alternatives |
Host | Rabbit Alternatives |
Clonality | |
Conjugate | This PDGFB antibody is un-conjugated Alternatives |
Application |
Immunohistochemistry (IHC), Western Blotting (WB)
Alternatives
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1 reference available |
Supplier | Log in to see |
Product Details anti-PDGFB AntibodyTarget Details PDGFB Application Details Handling References for anti-PDGFB antibody (ABIN2787714) Images |
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Blocking Peptide | Blocking peptide for this product available: ABIN981524 |
Sequence | NRCWALFLSL CCYLRLVSAE GDPIPEELYE MLSDHSIRSF DDLQRLLHGD |
Predicted Reactivity | Cow: 100%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Sheep: 100% |
Characteristics | This is a rabbit polyclonal antibody against PDGFB. It was validated on Western Blot using a cell lysate as a positive control. |
Purification | Affinity Purified |
Immunogen | The immunogen is a synthetic peptide directed towards the N terminal region of human PDGFB |
Target Details PDGFBProduct Details anti-PDGFB Antibody Application Details Handling References for anti-PDGFB antibody (ABIN2787714) Images back to top |
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Antigen | |
Alternative Name | PDGFB (PDGFB Antibody Abstract) |
Background |
PDGFB is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor.The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two splice variants have been identified for this gene. Alias Symbols: FLJ12858, PDGF2, SIS, SSV, c-sis Protein Interaction Partner: COL6A1, COL5A1, COL4A1, COL3A1, COL2A1, COL1A2, COL1A1, LYVE1, NRP1, ADIPOQ, MDFI, PDAP1, ART1, PDGFB, HRAS, THBS1, PDGFRA, PDGFRB, HSPG2, LRP1, A2M, SPARC, Protein Size: 241 |
Molecular Weight | 27 kDa |
Gene ID | 5155 |
NCBI Accession | NM_002608, NP_002599 |
UniProt | P01127 |
Pathways | RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling |
Application DetailsProduct Details anti-PDGFB Antibody Target Details PDGFB Handling References for anti-PDGFB antibody (ABIN2787714) Images back to top |
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Application Notes | Optimal working dilutions should be determined experimentally by the investigator. |
Comment |
Antigen size: 241 AA |
Restrictions | For Research Use only |
HandlingProduct Details anti-PDGFB Antibody Target Details PDGFB Application Details References for anti-PDGFB antibody (ABIN2787714) Images back to top |
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Format | Liquid |
Concentration | Lot specific |
Buffer | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose. |
Preservative | Sodium azide |
Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Handling Advice | Avoid repeated freeze-thaw cycles. |
Storage | -20 °C |
Storage Comment | For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. |
References for anti-PDGFB antibody (ABIN2787714)Product Details anti-PDGFB Antibody Target Details PDGFB Application Details Handling Images back to top |
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Background publications |
Chadjichristos, Morel, Derouette, Sutter, Roth, Brisset, Bochaton-Piallat, Kwak: "Targeting connexin 43 prevents platelet-derived growth factor-BB-induced phenotypic change in porcine coronary artery smooth muscle cells." in: Circulation research, Vol. 102, Issue 6, pp. 653-60, 2008 |
ImagesProduct Details anti-PDGFB Antibody Target Details PDGFB Application Details Handling References for anti-PDGFB antibody (ABIN2787714) back to top |
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Supplier Images |