anti-Human PDGFB antibody for Cytometry by Time of Flight

Recommended PDGFB Antibody (supplied by: Log in to see )

Antigen
Platelet-Derived Growth Factor beta Polypeptide (PDGFB) Antibodies
  • PDGF-2
  • PDGF2
  • SIS
  • SSV
  • c-sis
  • pdgf2
  • sis
  • si:ch211-79m20.1
  • si:dkey-261d11.1
  • PDGF-B
  • Sis
  • platelet derived growth factor subunit B
  • platelet-derived growth factor beta polypeptide
  • platelet derived growth factor subunit B S homeolog
  • platelet-derived growth factor beta polypeptide a
  • platelet derived growth factor, B polypeptide
  • PDGFB
  • pdgfb.S
  • pdgfba
  • Pdgfb
Reactivity
Human
112
87
52
3
3
3
2
1
Host
Mouse
125
26
5
3
Clonality
Monoclonal
Conjugate
This PDGFB antibody is un-conjugated
9
7
5
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
130
52
34
32
17
13
8
8
6
6
4
3
2
2
2
1
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Catalog No. ABIN258832
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General

Antigen Platelet-Derived Growth Factor beta Polypeptide (PDGFB) Antibodies
Reactivity Human
(112), (87), (52), (3), (3), (3), (2), (1)
Host Mouse
(125), (26), (5), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PDGFB antibody is un-conjugated
(9), (7), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(130), (52), (34), (32), (17), (13), (8), (8), (6), (6), (4), (3), (2), (2), (2), (1)
Pubmed 1 reference available
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Product Details anti-PDGFB Antibody

Target Details PDGFB Application Details Handling References for anti-PDGFB antibody (ABIN258832) Images
Specificity This antibody shows approximately some degree (40 % ) cross-reactivity with rPDGF-AB and no cross-reactivity with recombinant PDGF-AA.
Purification Protein G purified
Sterility 0.2 μm filtered
Immunogen This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with human PDGF BB recombinant protein.
Clone MM0014-5F66
Isotype IgG1
Plasmids, Primers & others

Target Details PDGFB

Product Details anti-PDGFB Antibody Application Details Handling References for anti-PDGFB antibody (ABIN258832) Images back to top
Antigen
Alternative Name PDGF-B (PDGFB Antibody Abstract)
Background Gene Symbol: PDGFB
Gene ID 5155
UniProt P01127
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling

Application Details

Product Details anti-PDGFB Antibody Target Details PDGFB Handling References for anti-PDGFB antibody (ABIN258832) Images back to top
Application Notes Western Blot 1:1000, Flow Cytometry 1:10-1:1000, Immunohistochemistry 1:50-1:200, Immunohistochemistry-Paraffin 1:50-1:200, CyTOF-readyFlow Cytometry was reported in scientific literature. This antibody shows approximately some degree (40 % ) cross-reactivity with rPDGF-AB and no cross-reactivity with recombinant PDGF-AA. Proteinase K (20 μg/mL in PBS) is recommended for antigen retrieval.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-PDGFB Antibody Target Details PDGFB Application Details References for anti-PDGFB antibody (ABIN258832) Images back to top
Format Lyophilized
Reconstitution Reconstitute with 0.5 mL sterilized PBS to a final concentration of 0.2 mg/mL.
Buffer A 0.2μm filtered solution in PBS
Buffer contains: No Preservative
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

References for anti-PDGFB antibody (ABIN258832)

Product Details anti-PDGFB Antibody Target Details PDGFB Application Details Handling Images back to top
Product cited in:

Schito, Rey, Tafani, Zhang, Wong, Russo, Russo, Semenza: "Hypoxia-inducible factor 1-dependent expression of platelet-derived growth factor B promotes lymphatic metastasis of hypoxic breast cancer cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 40, pp. E2707-16, 2012 (Sample species: Human). Further details: Western Blotting,Flow Cytometry

Images

Product Details anti-PDGFB Antibody Target Details PDGFB Application Details Handling References for anti-PDGFB antibody (ABIN258832) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Platelet-Derived Growth Factor beta Polypeptide (PDGFB) antibody (ABIN258832) Immunohistochemistry-Paraffin: PDGFB Antibody (MM0014-5F66) [ABIN2588320] - Human pla...