anti-Human PDGFBB antibody for ELISA

Recommended PDGFBB Antibody (supplied by: Log in to see )

Antigen
Platelet Derived Growth Factor BB (PDGFBB) Antibodies
  • PDGF-2
  • PDGF2
  • SIS
  • SSV
  • c-sis
  • platelet derived growth factor subunit B
  • PDGFB
Reactivity
Human
57
21
18
3
3
1
1
1
1
1
Host
Rabbit
66
5
2
1
Clonality
Polyclonal
Conjugate
This PDGFBB antibody is un-conjugated
10
6
2
2
2
2
1
1
Application
ELISA, Neutralization (Neut), Western Blotting (WB)
67
32
25
9
6
6
5
4
2
2
2
2
1
1
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Catalog No. ABIN465589
$ 493.17
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2889248 ELISA WB Rabbit IgG AA 16-65 Log in to see Polyclonal 0
1 ABIN465516 ELISA WB Biotin Rabbit Log in to see Polyclonal 0
1 ABIN205671 ELISA Func IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN3201470 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 82-190 Log in to see 0
1 ABIN203660 ELISA Rabbit AA 227-241 Log in to see Polyclonal 0
1 ABIN2583004 ELISA WB Biotin Rabbit IgG Log in to see Polyclonal 0
1 ABIN1922535 ELISA WB APC Rabbit IgG AA 212-241 Log in to see Polyclonal 0
1 ABIN1922537 ELISA WB PE Rabbit IgG AA 212-241 Log in to see Polyclonal 0
1 ABIN1922534 ELISA WB Alkaline Phosphatase (AP) Rabbit IgG AA 212-241 Log in to see Polyclonal 0
1 ABIN1922536 ELISA WB FITC Rabbit IgG AA 212-241 Log in to see Polyclonal 0
1 ABIN2583005 ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1836604 IF ELISA WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN354698 ELISA Sheep Log in to see Polyclonal 0
1 ABIN1922538 ELISA WB HRP Rabbit IgG AA 212-241 Log in to see Polyclonal 0
1 ABIN2583002 ELISA WB Rabbit IgG AA 212-241 Log in to see Polyclonal 0
1 ABIN205942 Func ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2337057 ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2337056 ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2337059 ELISA WB Biotin Rabbit IgG Log in to see Polyclonal 0
1 ABIN2337053 Neut IHC ELISA WB HRP Rabbit IgG Log in to see Polyclonal 0

General

Antigen Platelet Derived Growth Factor BB (PDGFBB) Antibodies
Reactivity Human
(57), (21), (18), (3), (3), (1), (1), (1), (1), (1)
Host Rabbit
(66), (5), (2), (1)
Clonality
Polyclonal
Conjugate This PDGFBB antibody is un-conjugated
(10), (6), (2), (2), (2), (2), (1), (1)
Application ELISA, Neutralization (Neut), Western Blotting (WB)
(67), (32), (25), (9), (6), (6), (5), (4), (2), (2), (2), (2), (1), (1)
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Product Details anti-PDGFBB Antibody

Target Details PDGFBB Application Details Handling Images
Specificity Human PDGF-BB
Purification Immunoaffinity purified
Immunogen E.coli derived recombinant Human PDGF-BB.

Type of Immunogen: Cell extract
Plasmids, Primers & others

Target Details PDGFBB

Product Details anti-PDGFBB Antibody Application Details Handling Images back to top
Antigen
Alternative Name PDGF-BB (PDGFBB Antibody Abstract)
Background Name/Gene ID: PDGFB
Family: PDGF

Synonyms: PDGFB, Becaplermin, PDGF-BB, PDGF-b, SSV, PDGF subunit B, PDGF-2, PDGF2, Proto-oncogene c-Sis, SIS, C-sis, PDGF, B chain
Gene ID 5155
UniProt P01127
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling

Application Details

Product Details anti-PDGFBB Antibody Target Details PDGFBB Handling Images back to top
Application Notes Approved: ELISA, Neut (3 - 4 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1 μg/mL overnight at 4 °C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hPDGF-BB (2.5 ng/mL), a concentration of 0.03-0.04 μg/mL of this antibody is required. ELISA: To detect hPDGF-BB by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human PDGF-BB (LS-C104638) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hPDGF-BB. Western Blot: To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Comment

Target Species of Antibody: Human

Restrictions For Research Use only

Handling

Product Details anti-PDGFBB Antibody Target Details PDGFBB Application Details Images back to top
Format Lyophilized
Reconstitution Sterile water to a concentration of 0.1-1.0 mg/mL
Concentration Lot specific
Buffer Lyophilized from PBS,  pH 7.2
Handling Advice Avoid freeze/thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Images

Product Details anti-PDGFBB Antibody Target Details PDGFBB Application Details Handling back to top
Supplier Images
 image for anti-Platelet Derived Growth Factor BB (PDGFBB) antibody (ABIN465589) anti-Platelet Derived Growth Factor BB (PDGFBB) antibody
 image for anti-Platelet Derived Growth Factor BB (PDGFBB) antibody (ABIN465589) anti-Platelet Derived Growth Factor BB (PDGFBB) antibody (Image 2)
 image for anti-Platelet Derived Growth Factor BB (PDGFBB) antibody (ABIN465589) anti-Platelet Derived Growth Factor BB (PDGFBB) antibody (Image 3)
 image for anti-Platelet Derived Growth Factor BB (PDGFBB) antibody (ABIN465589) anti-Platelet Derived Growth Factor BB (PDGFBB) antibody (Image 4)
 image for anti-Platelet Derived Growth Factor BB (PDGFBB) antibody (ABIN465589) anti-Platelet Derived Growth Factor BB (PDGFBB) antibody (Image 5)