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|Antigen||Platelet Derived Growth Factor BB (PDGFBB) Antibodies|
|Conjugate||This PDGFBB antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut), ELISA, Western Blotting (WB)
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Product Details anti-PDGFBB AntibodyTarget Details PDGFBB Application Details Handling Images
E.coli derived recombinant Human PDGF-BB.
Type of Immunogen: Cell extract
Target Details PDGFBBProduct Details anti-PDGFBB Antibody Application Details Handling Images back to top
|Alternative Name||PDGF-BB (PDGFBB Antibody Abstract)|
Name/Gene ID: PDGFB
Synonyms: PDGFB, Becaplermin, PDGF-BB, PDGF-b, SSV, PDGF subunit B, PDGF-2, PDGF2, Proto-oncogene c-Sis, SIS, C-sis, PDGF, B chain
|Pathways||RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling|
Application DetailsProduct Details anti-PDGFBB Antibody Target Details PDGFBB Handling Images back to top
Approved: ELISA, IHC-P (1 μg/mL), Neut (3 - 4 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1 μg/mL overnight at 4 °C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hPDGF-BB (2.5 ng/mL), a concentration of 0.03-0.04 μg/mL of this antibody is required. ELISA: To detect hPDGF-BB by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human PDGF-BB (LS-C104638) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hPDGF-BB. Western Blot: To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PDGFBB Antibody Target Details PDGFBB Application Details Images back to top
|Reconstitution||Sterile water to a concentration of 0.1-1.0 mg/mL|
|Buffer||Lyophilized from PBS, pH 7.2|
|Handling Advice||Avoid freeze/thaw cycles.|
|Storage||4 °C,-20 °C|
Store Lyophilized at room temperature up to 1 month
Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
ImagesProduct Details anti-PDGFBB Antibody Target Details PDGFBB Application Details Handling back to top