anti-Human PDGFRA antibody for Western Blotting

Recommended PDGFRA Antibody (supplied by: Log in to see )

Antigen
Platelet-Derived Growth Factor Receptor, alpha Polypeptide (PDGFRA) Antibodies
  • AI115593
  • CD140a
  • Pdgfr-2
  • APDGFR
  • PDGFACE
  • CD140A
  • PDGFR-2
  • PDGFR2
  • RHEPDGFRA
  • PDGFR
  • PDGFR-A
  • platelet derived growth factor receptor alpha
  • platelet derived growth factor receptor, alpha polypeptide
  • platelet-derived growth factor receptor, alpha polypeptide L homeolog
  • platelet-derived growth factor receptor, alpha polypeptide
  • PDGFRA
  • Pdgfra
  • pdgfra.L
Epitope
AA 698-727, Tyr720
37
22
20
20
19
18
18
17
17
15
15
15
15
14
12
11
11
10
10
10
7
7
6
5
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
518
279
184
6
6
6
5
5
3
3
3
2
2
1
1
1
1
Host
Rabbit
376
169
50
3
1
Clonality
Polyclonal
Conjugate
This PDGFRA antibody is un-conjugated
36
32
32
28
23
14
13
12
7
7
7
7
6
5
5
5
5
5
5
5
5
5
5
5
4
4
3
3
3
2
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
364
233
222
142
114
67
56
51
15
15
14
14
9
3
3
2
1
1
1
1
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Catalog No. ABIN392024
$ 335.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0748372 ABIN3043897 IHC (p) WB Rabbit IgG AA 968-1002, C-Term Log in to see Polyclonal 0
3.0748372 ABIN392027 IHC (p) WB Rabbit Ig Fraction AA 746-775, Tyr768 Log in to see Polyclonal 5
3.0748372 ABIN392029 IHC (p) WB Rabbit Ig Fraction AA 732-761, Tyr754 Log in to see Polyclonal 5
3.0748372 ABIN392025 IHC (p) WB Rabbit Ig Fraction AA 740-769, Tyr762 Log in to see Polyclonal 5
3.0748372 ABIN4881219 ELISA FACS ICC WB Mouse IgG1 AA 361-528 Log in to see 8E12F2 0
3.0748372 ABIN2705108 IC IF WB Rabbit C-Term Log in to see Polyclonal 0
3.0748372 ABIN1532090 ELISA WB Rabbit IgG AA 816-865, pTyr849 Log in to see Polyclonal 2
3.0748372 ABIN1533127 ELISA WB Rabbit IgG AA 816-865 Log in to see Polyclonal 2
3.0748372 ABIN446762 ICC IF IHC (p) IHC WB Rabbit Log in to see Polyclonal 2
3.0748372 ABIN1533378 IF IHC ELISA WB Rabbit IgG AA 1031-1080 Log in to see Polyclonal 0
3.0748372 ABIN392022 FACS IHC (p) WB Rabbit Ig Fraction AA 1048-1077, C-Term Log in to see Polyclonal 1
3.0748372 ABIN1869734 ICC IHC WB Rabbit IgG AA 170-387 Log in to see Polyclonal 0
3.0748372 ABIN271391 WB Rabbit pTyr742 Log in to see Polyclonal 3
3.0748372 ABIN5611263 ELISA FACS IHC WB Mouse IgG2a AA 179-361 Log in to see 5B11G5 0
3.0748372 ABIN5611262 ELISA FACS WB Mouse IgG1 AA 179-361 Log in to see 1A10A8 0
3.0748372 ABIN5949588 FACS ICC IF IHC IHC (p) WB Rabbit IgG N-Term Log in to see JF104-6 0
3.0748372 ABIN3044258 IHC (p) WB Rabbit IgG AA 1030-1043, C-Term Log in to see Polyclonal 0
3.0748372 ABIN4344505 CyTOF ELISA FACS ICC IF WB Mouse Log in to see 8E12F2 0
3.0748372 ABIN499026 WB Rabbit Log in to see Polyclonal 0
3.0748372 ABIN498160 IF IHC (p) WB Rabbit Log in to see Polyclonal 0

General

Antigen Platelet-Derived Growth Factor Receptor, alpha Polypeptide (PDGFRA) Antibodies
Epitope AA 698-727, Tyr720
(37), (22), (20), (20), (19), (18), (18), (17), (17), (15), (15), (15), (15), (14), (12), (11), (11), (10), (10), (10), (7), (7), (6), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(518), (279), (184), (6), (6), (6), (5), (5), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(376), (169), (50), (3), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This PDGFRA antibody is un-conjugated
(36), (32), (32), (28), (23), (14), (13), (12), (7), (7), (7), (7), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (2), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(364), (233), (222), (142), (114), (67), (56), (51), (15), (15), (14), (14), (9), (3), (3), (2), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-PDGFRA Antibody

Target Details PDGFRA Application Details Handling References for anti-PDGFRA antibody (ABIN392024) Images
Specificity This PDGFRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y720 of human PDGFRA.
Predicted Reactivity Chicken,Mouse (Murine),Rat (Rattus),Xenopus laevis
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This PDGFRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 698-727 AA from human PDGFRA.
Clone RB11101
Isotype Ig Fraction
Plasmids, Primers & others

Target Details PDGFRA

Product Details anti-PDGFRA Antibody Application Details Handling References for anti-PDGFRA antibody (ABIN392024) Images back to top
Antigen
Alternative Name PDGFRA (PDGFRA Antibody Abstract)
Background PDGFRA is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.
Synonyms: PDGF-R-alpha, CD140a antigen, Alpha platelet-derived growth factor receptor
Molecular Weight 122669 DA
Gene ID 5156
NCBI Accession NP_006197
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Platelet-derived growth Factor Receptor Signaling

Application Details

Product Details anti-PDGFRA Antibody Target Details PDGFRA Handling References for anti-PDGFRA antibody (ABIN392024) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-PDGFRA Antibody Target Details PDGFRA Application Details References for anti-PDGFRA antibody (ABIN392024) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-PDGFRA antibody (ABIN392024)

Product Details anti-PDGFRA Antibody Target Details PDGFRA Application Details Handling Images back to top
Background publications

Wilczynski, Chen, Chen, Howell, Shively, Alberts: "Expression and mutational analysis of tyrosine kinase receptors c-kit, PDGFRalpha, and PDGFRbeta in ovarian cancers." in: Human pathology, Vol. 36, Issue 3, pp. 242-9, 2005

Hiwatari, Taki, Tsuchida, Hanada, Hongo, Sako, Hayashi: "Novel missense mutations in the tyrosine kinase domain of the platelet-derived growth factor receptor alpha(PDGFRA) gene in childhood acute myeloid leukemia with t(8;21)(q22;q22) or inv(16)(p13q22)." in: Leukemia, Vol. 19, Issue 3, pp. 476-7, 2005

Debiec-Rychter, Cools, Dumez, Sciot, Stul, Mentens, Vranckx, Wasag, Prenen, Roesel, Hagemeijer, Van Oosterom, Marynen: "Mechanisms of resistance to imatinib mesylate in gastrointestinal stromal tumors and activity of the PKC412 inhibitor against imatinib-resistant mutants." in: Gastroenterology, Vol. 128, Issue 2, pp. 270-9, 2005

Subramanian, West, Corless, Ou, Rubin, Chu, Leung, Yuen, Zhu, Hernandez-Boussard, Montgomery, Nielsen, Patel, Goldblum, Heinrich, Fletcher, van de Rijn: "Gastrointestinal stromal tumors (GISTs) with KIT and PDGFRA mutations have distinct gene expression profiles." in: Oncogene, Vol. 23, Issue 47, pp. 7780-90, 2004

Sakurai, Hasegawa, Sakuma, Takazawa, Motegi, Nakajima, Saito, Fukayama, Shimoda: "Myxoid epithelioid gastrointestinal stromal tumor (GIST) with mast cell infiltrations: a subtype of GIST with mutations of platelet-derived growth factor receptor alpha gene." in: Human pathology, Vol. 35, Issue 10, pp. 1223-30, 2004

Images

Product Details anti-PDGFRA Antibody Target Details PDGFRA Application Details Handling References for anti-PDGFRA antibody (ABIN392024) back to top
Supplier Images
Western Blotting (WB) image for anti-Platelet-Derived Growth Factor Receptor, alpha Polypeptide (PDGFRA) (AA 698-727), (Tyr720) antibody (ABIN392024) Western blot analysis of PDGFRA (arrow) using rabbit polyclonal PDGFRA Antibody (Y720...
Immunohistochemistry (IHC) image for anti-Platelet-Derived Growth Factor Receptor, alpha Polypeptide (PDGFRA) (AA 698-727), (Tyr720) antibody (ABIN392024) Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PDGFRA...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Platelet-Derived Growth Factor Receptor, alpha Polypeptide (PDGFRA) (AA 698-727), (Tyr720) antibody (ABIN392024) ABIN392024,ABIN2841799 staining PDGFRA in Human hepatic carcinoma tissue sections by ...
Western Blotting (WB) image for anti-Platelet-Derived Growth Factor Receptor, alpha Polypeptide (PDGFRA) (AA 698-727), (Tyr720) antibody (ABIN392024) Anti-PDGFRA-pY720. ctrlat 1:1000 dilution + human platelet lysates Lysates/proteins a...