anti-Mouse (Murine) RYK antibody for Western Blotting

Recommended RYK Antibody (supplied by: Log in to see )

Antigen
RYK Receptor-Like Tyrosine Kinase (RYK) Antibodies
  • RYK
  • jtk5
  • ryk1
  • jtk5a
  • d3s3195
  • wu:fb37b12
  • wu:fi05b06
  • zgc:158381
  • MGC114645
  • D3S3195
  • JTK5
  • JTK5A
  • RYK1
  • AW536699
  • ERK-3
  • Vik
  • receptor-like tyrosine kinase
  • receptor-like tyrosine kinase S homeolog
  • tyrosine-protein kinase RYK
  • RYK
  • ryk
  • ryk.S
  • LOC100541106
  • Ryk
Epitope
AA 160-190
36
11
11
11
10
10
10
8
7
7
7
3
3
3
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
126
77
59
3
2
2
1
1
1
1
Host
Rabbit
112
23
11
5
Clonality
Polyclonal
Conjugate
This RYK antibody is un-conjugated
11
11
10
10
10
10
2
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
144
72
58
10
4
4
3
2
2
1
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Catalog No. ABIN392050
$ 385.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
15.251959 ABIN4900273 CyTOF FACS ICC WB Sheep IgG AA 35-211 Log in to see Polyclonal 1
12.811565 ABIN6390068 ELISA WB Rabbit IgG AA 150-230 Log in to see Polyclonal 0
12.811565 ABIN5651101 IHC WB Atto 488 Rabbit AA 150-250 Log in to see Polyclonal 0
12.811565 ABIN5651100 IHC WB Atto 390 Rabbit AA 150-250 Log in to see Polyclonal 0
12.811565 ABIN5651106 IHC WB Atto 680 Rabbit AA 150-250 Log in to see Polyclonal 0
12.811565 ABIN5651105 IHC WB Atto 655 Rabbit AA 150-250 Log in to see Polyclonal 0
12.811565 ABIN5651113 IHC WB PerCP Rabbit AA 150-250 Log in to see Polyclonal 0
12.811565 ABIN5651104 IHC WB Atto 633 Rabbit AA 150-250 Log in to see Polyclonal 0
12.811565 ABIN5651110 IHC WB FITC Rabbit AA 150-250 Log in to see Polyclonal 0
12.811565 ABIN5651111 IHC WB HRP Rabbit AA 150-250 Log in to see Polyclonal 0
12.811565 ABIN5651115 IHC WB Streptavidin Rabbit AA 150-250 Log in to see Polyclonal 0
12.811565 ABIN5651103 IHC WB Atto 594 Rabbit AA 150-250 Log in to see Polyclonal 0
12.811565 ABIN5651112 IHC WB PE-Atto 594 Rabbit AA 150-250 Log in to see Polyclonal 0
12.811565 ABIN5651099 IHC WB APC Rabbit AA 150-250 Log in to see Polyclonal 0
12.811565 ABIN5651107 IHC WB Atto 700 Rabbit AA 150-250 Log in to see Polyclonal 0
12.811565 ABIN5651114 IHC WB PE Rabbit AA 150-250 Log in to see Polyclonal 0
12.811565 ABIN5651102 IHC WB Atto 565 Rabbit AA 150-250 Log in to see Polyclonal 0
12.811565 ABIN5651108 IHC WB Alkaline Phosphatase (AP) Rabbit AA 150-250 Log in to see Polyclonal 0
12.811565 ABIN5651109 IHC WB Biotin Rabbit AA 150-250 Log in to see Polyclonal 0
12.811565 ABIN5651098 IHC WB Rabbit AA 150-250 Log in to see Polyclonal 0

General

Antigen RYK Receptor-Like Tyrosine Kinase (RYK) Antibodies
Epitope AA 160-190
(36), (11), (11), (11), (10), (10), (10), (8), (7), (7), (7), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(126), (77), (59), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(112), (23), (11), (5)
Clonality (Clone)
Polyclonal   ( )
Conjugate This RYK antibody is un-conjugated
(11), (11), (10), (10), (10), (10), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(144), (72), (58), (10), (4), (4), (3), (2), (2), (1)
Pubmed 4 references available
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Product Details anti-RYK Antibody

Target Details RYK Application Details Handling References for anti-RYK antibody (ABIN392050) Images
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This RYK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-190 AA from human RYK.
Clone RB1491
Isotype Ig Fraction
Plasmids, Primers & others

Target Details RYK

Product Details anti-RYK Antibody Application Details Handling References for anti-RYK antibody (ABIN392050) Images back to top
Antigen
Alternative Name RYK (RYK Antibody Abstract)
Background Function:
May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C- terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development.

Cellular Location:
Membrane, Single-pass type I membrane protein (By similarity). Nucleus (By similarity). Cytoplasm (By similarity). Note=In cells that have undergone neuronal differentiation, the C-terminal cleaved part is translocated from the cytoplasm to the nucleus (By similarity)

Tissue Location:
Observed in all the tissues examined.

Synonyms: Tyrosine-protein kinase RYK precursor
Molecular Weight 67507 Da
Gene ID 6259
UniProt P34925
Pathways RTK Signaling, WNT Signaling, Regulation of Cell Size

Application Details

Product Details anti-RYK Antibody Target Details RYK Handling References for anti-RYK antibody (ABIN392050) Images back to top
Application Notes Suggested dilutions:
WB = 1:1000, IHC (p) = 1:50-100
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-RYK Antibody Target Details RYK Application Details References for anti-RYK antibody (ABIN392050) Images back to top
Format Liquid
Concentration 1.57 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months

References for anti-RYK antibody (ABIN392050)

Product Details anti-RYK Antibody Target Details RYK Application Details Handling Images back to top
Product cited in:

Povinelli, Nemeth: "Wnt5a regulates hematopoietic stem cell proliferation and repopulation through the Ryk receptor." in: Stem cells (Dayton, Ohio), Vol. 32, Issue 1, pp. 105-15, 2014

González, Fernández-Martos, Arenas, Rodríguez: "The Ryk receptor is expressed in glial and fibronectin-expressing cells after spinal cord injury." in: Journal of neurotrauma, Vol. 30, Issue 10, pp. 806-17, 2013 Method employed by authors: Immunofluorescence (IF)

Santiago, Oguma, Brown, Laurence: "Noncanonical Wnt signaling promotes osteoclast differentiation and is facilitated by the human immunodeficiency virus protease inhibitor ritonavir." in: Biochemical and biophysical research communications, Vol. 417, Issue 1, pp. 223-30, 2012 Method employed by authors: Western Blotting (WB)

Miyashita, Koda, Kitajo, Yamazaki, Takahashi, Kikuchi, Yamashita: "Wnt-Ryk signaling mediates axon growth inhibition and limits functional recovery after spinal cord injury." in: Journal of neurotrauma, Vol. 26, Issue 7, pp. 955-64, 2009

Images

Product Details anti-RYK Antibody Target Details RYK Application Details Handling References for anti-RYK antibody (ABIN392050) back to top
Supplier Images
Western Blotting (WB) image for anti-RYK Receptor-Like Tyrosine Kinase (RYK) (AA 160-190) antibody (ABIN392050) The anti-RYK Pab (ABIN392050) is used in Western blot to detect RYK in Jurkat cell ly...
Western Blotting (WB) image for anti-RYK Receptor-Like Tyrosine Kinase (RYK) (AA 160-190) antibody (ABIN392050) Western blot analysis of RYK (arrow) using rabbit polyclonal RYK Antibody (ABIN392050...
Immunohistochemistry (IHC) image for anti-RYK Receptor-Like Tyrosine Kinase (RYK) (AA 160-190) antibody (ABIN392050) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Western Blotting (WB) image for anti-RYK Receptor-Like Tyrosine Kinase (RYK) (AA 160-190) antibody (ABIN392050) Western blot analysis of lysate from mouse NIH/3T3 cell line, using RYK Antibody (N17...