We are preparing the requested document. Please wait, this may take a while...!
|Antigen||SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) Antibodies|
|Reactivity||Dog (Canine), Human, Mouse (Murine) Alternatives|
|Conjugate||This SHC1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-SHC1 AntibodyTarget Details SHC1 Application Details Handling Images
|Specificity||This antibody specifically recognizes shc/p66 when it is phosphorylated at Serine 36. We recommend to Immunoprecipitate shc/p66 prior to detection with mab shc/p66-6E10.|
|Characteristics||Synonyms: SHC-transforming protein 1, SHC-transforming protein A, SHC-transforming protein 3,SHCA, Src homology 2 domain-containing-transforming protein C1|
|Purification||Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.|
|Components||incl. positive Control|
|Immunogen||Synthetic phosphopeptide conjugated to KLH|
Target Details SHC1Product Details anti-SHC1 Antibody Application Details Handling Images back to top
|Alternative Name||SHC1 / SHC (SHC1 Antibody Abstract)|
|Background||Mammalian cells can express three alternatively spliced isoforms of the shc adaptor protein: shc/p46, shc/p52 and shc/p66. shc/p66 contains a unique N-terminal protein domain. In addition to tyrosine phosphorylation of Tyr 239/240 and/or Tyr 317, shc/p66 is phosphorylated at serine 36, e.g. in response to EGF. Serine phosphorylation of shc/p66 impairs its ability to bind to the activated EGF receptor thus inhibiting EGF receptor downstream signalling pathways.Synonyms: SHC-transforming protein 1, SHC-transforming protein 3, SHC-transforming protein A, SHCA, Src homology 2 domain-containing-transforming protein C1|
|Molecular Weight||66 kDa|
|Pathways||RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, ER-Nucleus Signaling, Signaling Events mediated by VEGFR1 and VEGFR2|
Application DetailsProduct Details anti-SHC1 Antibody Target Details SHC1 Handling Images back to top
Western Blot: 1 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: shc/p66 precipitated from EGF-treated HepG2 cells (See included Protocolbelow). ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Protocol||Positive Control Cell Lysate: HepG2 shc/p66-IPFormulationshc/p66 immunoprecipitated with anti-shc from EGF stimulated HepG2 cells. Proteins wereeluted with SDS and lyophilized from PBS/SDS. ReconstitutionReconstitute by addition of 125 µl H2O. After complete solubilization add 125 µl 2xSDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Aliquote and store frozen. Avoid repeated freezing and thawing.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-SHC1 Antibody Target Details SHC1 Application Details Images back to top
|Reconstitution||Restore with 1 mL H2O (15 min, RT).|
|Buffer||1 mL2 x PBS, 0.09 % Sodium Azide, PEG and Sucrose|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||-20 °C/-80 °C|
Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
Shelf life: one year from despatch.
|Expiry Date||12 months|
ImagesProduct Details anti-SHC1 Antibody Target Details SHC1 Application Details Handling back to top